Adaptations of plant responses in Agave sisalana under drought stress conditions

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Research Paper 01/10/2016
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Adaptations of plant responses in Agave sisalana under drought stress conditions

Sania Riaz, Beenish Aftab, Muhammad Bilal Sarwar, Fatima Batool, Fozia Iqbal, Zarnab Ahmad, Bushra Rashid, Tayyab Husnain
J. Bio. Env. Sci.9( 4), 114-123, October 2016.
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This study was aimed to characterize the drought tolerance at 10 and 2% field capacity in a desert plant Agave sisalana during a period of 2 months. Physiological, biochemical and water related attributes were investigated in order to explore agave plant’s survival under water stressed conditions. Green house experiment was performed in CRD with four replications of drought stress imposed on six months old progeny of agave plants. Drought stress significantly decreased the photosynthetic rate (1.45 µmol m-2 s-1), transpiration rate (0.15 mmol m-2 s-1), stomatal conductance (0.9 µmol m-2 s-1), leaf area (12.9 cm2), relative water content (30.7%) and total chlorophyll content (0.24 mg g-1). The concentration of biochemical indicators such as proline (4.26 µg g-l) and Melondialdehyde (0.99 µmol g-1 FW) were increased by the increase in drought stress time from 10 to 2% field capacity. The correlation coefficients (r) between physiological, biochemical and water related traits were also found to be significant and showed positive and negative association among each other. This knowledge of stress inducible responses generated in the form of physiochemical attributes in water deficit crops can serve to be very useful in the future for better understanding of metabolic and gene regulatory pathways.


Anjum SA, Xie X, Wang L, Saleem MF, Man C, Lei W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6, 2026-2032.

Anjum SA, Wang LC, Farooq M, Khanand I, Xue LL. 2011b. Methyl jasmonate-induced alteration in lipid peroxidation, antioxidative defense system and yield in soybean under drought. Journal of Agronomyand Crop Science 197, 296-301.

Araus JL, Slafer GA, Reynolds MP, Royo C. 2002. Plant breeding and drought in C3 cereals: What should we breed for? Annals of Botany 89, 925-940.

Arnon D, Whatley F. 1949. Is chloride a coenzyme of photosynthesis? Science 2865, 554-556.

Ashraf M, Foolad MR. 2007. Roles of glycinebetaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59, 206-216.

Barrs HD, Weatherley PE. 1962. A reexamination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences 15, 413-428.

Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39, 205-207.

Chaves MM, Maroco JP, Pereira JS. 2003. Understanding plant response to drought from common bean (P. vulgaris), Journal of Experimental Botany 57, 1391-1398.

Demiral T, Turkan I. 2004. Does exogenous glycinebetaine affect antioxidative system of rice seedlings under Na Cl treatment? Journal of plant physiology 161, 1089-1110.

Flexas J, Medrano H. 2002. Drought-inhibition of photosynthesis in C-3 plants: Stomatal and nonstomatal limitation revisited. Annals of Botany 89, 183-189.

Guerfel M, Baccouri O, Boujnah D, Chaibi W, Zarrouk M. 2009. Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Oleaeuropaea L.) cultivars. Scientia Horticulturae 119, 257-263.

Hamada AM. 2000. Amelioration of drought stress by ascorbic acid, thiamine or aspirin in wheat plants. Indian Journal of Plant Physiology 5, 358-364.

Igathinathane C, Pordesimo LO, Columbus EP, Batchelor WD, Methuku SR. 2008. Shape identification and particles size distribution from basic shape parameters using Image. Computers and Electronics in Agriculture 2, 168-182.

Jabeen F, Shahbaz M, Ahraf M. 2008. Discriminating some prospective cultivars of maize (zea mays L.) for drought tolerance using gas exchange characteristics and proline contents as physiological markers. Pakistan Journal of Botany 40, 2329-2343.

Janson JG, Thomas GR, Mason-pharr D. 2004. Heat and drought influence photosynthesis, water relations, and soluble carbohydrates of two ecotypes of redbud (Cerciscanadensis). Journal of American Society of Horticultural Sciences 129, 497-502.

Mafakheri A, Siosemardeh B, Bahramnejad, Struik PC, Sohrabi Y. 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science 4, 500-585.

Manivannan P, Jaleel CA, Sankar B, Kishorekumar A, Somasundaram R, Alagu GM, Lakshmanan, Panneerselvam R. 2007b. Growth, biochemical modifications and proline metabolism in Helianthus annuusL. as induced by drought stress. Colloids and Surfaces B: Biointerfaces 59, 141-149.

Meloni DA, Olivamartinez MA, Cambraia J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany 49, 69-76.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7, 405-410.

Mohammed B, Gabel M, Karlsson LM. 2013. Nutritive values of the drought tolerant food and fodder crop enset. African Journal of Agricultural Research 8, 2326-2333.

Moussa HR. 2006. Influence of exogenous application of silicon on physiological response of salt-stressed maize (Zea mays L.). International Journal of Agriculture and Biology 8, 293-297.

Moussa HR, SAbdel-Aziz M. 2008. Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Australian Journal of Crop Science 1, 31-36.

Nath AK, Kumari S, Sharma DR. 2005. In vitro selection and characterization of water stress tolerant cultures of bell pepper. Indian Journal of Plant Physiology 10, 14-19.

Nayyar H, Gupta D. 2006. Differential sensitivity of C3 and C4 plants to water deficit stress association with oxidative stress and antioxidants. Environmental and Experimental Botany 58, 106-113.

Nobel PS. 1977. Water relations of flowering of Agave Desert. Botanical Gazette 138, 1-6.

Nyachiro JM, Briggs KG, Hoddinott J, Johnson-Flanagan AM. 2001. Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat. Cereal Research Communication 29, 135-142.

Prat H. 1948. General features of the epidermis in Zea mays. Annals of Missouri Botanical Garden 35, 341-351.

Rodriguez-Uribe L, O’Connell MA. 2006. A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolusacutifolius) and common bean (P. vulgaris). Journal of Experimental Botany 57, 1391-1398.

Sakhanokho HF, Ozias-akins P, May OL, Chee P. 2004. Induction of somatic embryogenesis and plant regeneration in select Georgia and Pee Dee cotton lines. Crop Science 44, 2199-2205.

Sankar B, Jaleel CA, Manivannan P, Kishore-kumar A, Somasundaram R, Panneer-selvam R. 2007. Drought-induced biochemical modifications and proline metabolism in Abelmoschusesculentus (L.) Moench. Acta Botanica Croatica 66, 43-56.

Selote DS, Chopra RK. 2004. Drought induced spike sterility is associated with an inefficient antioxidant defense in rice plants. Physiologia Planta-rum 121, 462-471.

Szabados L, Savoure A. 2009. Proline: a multif-unctional amino acid. Trends Plant Science 15, 89-97.

Vijayalakshmi T, Varalaxmi Y, Jainender S, Yadav SK, Vanaja M, Jyothilakshmi N, Maheswari M. 2012. Physiological and biochemical basis of water-deficit stress tolerance in pearl millet hybrid and parents. Journal of American Plant Science 3, 1730-1740. 

Wilhite DA, Svoboda MD, Hayes HJ. 2007. Understanding the complex impacts of drought: A key to enhancing drought mitigation and preparedness. Water Resource Management 21, 763-774.

Yadav SK, Jyothilakshmi N, Maheswari M, Vanaja M, Venkateswarlu B. 2005. Influence of water deficit at vegetative, anthesis and grain filling stages on water relation and grain yield in sorghum. Indian Journal of Plant Physiology 10, 20-24.

Yang F, Miao LF. 2010. Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica 44, 23-37.