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Research Paper | February 1, 2015

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Adventitious organogenesis induced in sweet orange (Citrus sinensis L.) var. “half-blood” maltese: morphogenetic and histological study

Kaouther Benmahmoud, Emna Jedidi, Asma Najar, Rachida Ghezel, Claire Kevers, Ahmed Jemmali, Nadhra Elloumi

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Int. J. Agron. Agri. Res.6(2), 1-7, February 2015

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Abstract

Tunisian citrus crops are faced to several abiotic and biotic constraints among which virus and virus-like diseases are incurable. The production of virus-free plants systematically needs the use of in vitro techniques. In this context, somatic embryogenesis and further plantlet regeneration of the Tunisian “half-blood” Maltese orange were obtained using explants consisting in style/stigma collected from unopened flowers.Somatic embryos were induced on Murashige and Skoog medium containing 13.3 µM 6-benzylaminopurine and 500 mg.l-1 malt extract, but their germination was obtained on hormone free-medium. Somatic embryogenesis was induced indirectly from intermediate friable callus initiated at the basal part of the style. Somatic embryos exhibited central procambial cells and were surrounded by a protoderm isolating them from the callus. These embryos had bipolar structure confirmed by the presence of shoot and root apices at cotyledonary stage. The use of cotyledon excised from those embryos failed to regenerate somatic embryos, but gave rise to direct organogenesis in two forms, true buds and protuberances both evolved in shoots after transfer in hormone-free medium. According to histological observations, protuberances are induced from epidermal and subepidermal cells of the cotyledon explant and remain closely attached to their mother tissue even at the shoot stage.

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Adventitious organogenesis induced in sweet orange (Citrus sinensis L.) var. “half-blood” maltese: morphogenetic and histological study

Carimi F. 2005. Somatic embryogenesis protocols: Citrus. In: Jain SM, Gupta PK, ed. Protocol for somatic embryogenesis in woody plants. Netherlands, 321- 343.

Carimi F, De Pasquale F, Crescimanno FG. 1994. Somatic embryogenesis from styles of lemon (Citrus limon). Plant Cell Tissue Organ Culture 37, 209- 211.

Carimi F, De Pasquale F, Crescimanno FG. 1999. Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Reports 18, 935- 940.

Carimi F, Tortorici MC, De Pasquale F, Crescimanno FG. 1998. Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group [Citrus sinensis (L.) Osb.]. Plant Cell Tissue Organ Culture 54, 183- 189.

Carvalho A, Santos FA, Machado MA. 2002. Psorosis virus complex elimination from citrus by shoot-tip grafting associated to thermotherapy. Fitop. Brasileira 27, 300-308.

D’Onghia AM, Carimi F, De Pasquale F, Djelouah K, Martelli GP. 2000. Somatic embryogenesis from style and stigma: a new technique for the sanitation, conservation and Safe exchange of Citrus germplasm. Proceedings of the 19th Congress of the International Society of Citriculture, Florida 147- 149.

De Pasquale F, Carimi F, Crescimanno FG. 1994. Somatic embryogenesis from styles of different cultivars of Citrus limon (L.) Burm. Australian Journal of Botany 42, 587- 594.

Haensch KT. 2004. Morpho-histological study of somatic embryo-like structures in hypocotyls cultures of Pelargonium x hortorum Bailey. Plant Cell Reports 22, 376- 381.

Lebdi Grissa K. 2010. Etude de base sur les cultures d’agrumes et de tomates en Tunisie. Regional Integrated Pest Management Program in the Near East, 13- 60.

Lombardo G, Alessandro R, Scialabba A, Sciandra M, De Pasquale F. 2011. Direct Organogenesis from Cotyledons in Cultivars of Citrus clementina Hort. Ex Tan. American Journal of Plant Sciences 2, 237- 244.

Meziane, M, Boudjeniba, M, Frasheri, D, D’Onghia, AM, Carra, A, Carimi, F. 2012. A first attempt on sanitation of Algerian Citrus genotypes by stigma/style somatic embryogenesis. Acta Horticulturea 940, 713- 718.

Miah MN, Islam S, Hadiuzzaman S. 2002. Regeneration of plantlets through somatic embryogenesis from nucellar tissue of Citrus macroptera Mont. Var. anammenis (‘Sat kara’). Plant Tissue Culture 12, 167-172.

Moreira-Dias JM, Molina RV, Bordón Y, Guardiola JL, García-Luis A. 2000. Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone re-quirements and in their response to light. Annals of Botany 85, 103- 110.

Murashige T, Skoog F. 1962. A Revised Medium for Rapid Growth and Bio Assays with tobacco Tissue Cultures. Physiologia Plantarum 15, 473- 497.

Navarro L, Juàrez J, Ballester JF, Pina JA. 1980. Elimination of some citrus pathogens producing psorosis-like leaf symptoms by shoot-tip grafting in vitro. Proceedings of the 8th Conference of the International Organization of Citrus Virologists. Sidney, 162- 166.

Puigderrajols P, Celestino C, Mònica S, Mariano T, Marisa M. 2000. Histology of organogenic and embryogenic responses in cotyledons of somatic embryos of quercus suber l. International Journal of Plant Sciences 161, 353- 362.

Rodriguez APM, Wetzstein HY. 1998. A morphological and histological comparison of the initiation and development of pecan (Carya illinoinensis) somatic embryogenic cultures induced with naphthaleneacetic acid or 2,4-dichlorophe-noxyacetic acid. Protoplasma 204, 71- 83.

Saini HK, Gill MS, Gill MIS. 2010. Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lusc.). Indian Journal of Biotechnology 9, 419 -423.

Salaj J, Petrovska B, Obert B, Prêtòvà A. 2005. Histological study of embryo-like structures initiated from hypocotyls segments of flax (Linum usitatissimum L.). Plant Cell Reports 24, 590-595.

Schinor EH, De Azevedo FA, Mourão Filho, FAA, Mendes BMJ. 2011. In vitro organogenesis in some citrus species. Revista Brasileira Fruticultura 33, 526- 531.

Tang H, Ren Z, Gabi K. 2000. Somatic embryogenesis and organogenesis from immature embryo cotyledons of three sour cherry cultivars (Prunus cerasus L.). Scientia Horticulturea 83, 109-126.

Zhou S, Brown DCW. 2006. High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants. Plant Cell Reports 25, 166- 173.

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