AFLP marker-based identification and genetic relationships of olive cultivars in the region of Hbebsa “North West of Tunisia”
Paper Details
AFLP marker-based identification and genetic relationships of olive cultivars in the region of Hbebsa “North West of Tunisia”
Abstract
In the region of Hbebsa, little is known about the olive germplasm, and even though there is an important olive biodiversity, studies about characterization and evaluation are scarce. The aim of this work was to make a molecular characterization by the use of amplified fragment length polymorphism (AFLP) markers. A total of 13 olive varieties were genotyped using different EcoRI–MseI AFLP primer combinations. Auto radiographs revealed 92 polymorphic markers in a total of 237 detected fragments. A set of redundant marker patterns was identified and deleted from the binary data matrix; data analysis demonstrated a high degree of polymorphism with an average of 35%. The analysis of AFLP profiles found in our set of olive cultivars showed a wide genetic diversity among olive germplasm. The UPGMA cluster analyses using Jaccard’s index and the Principal coordinate analysis (PCO) revealed that the genetic diversity was predominantly structured according to fruit size. The data obtained can be used for the varietal survey and construction of a database of olive varieties grown in the region of Hbebsa and providing also additional information that could form the basis for the national design of breeding programs.
Abaza L, Taamalli M, Ben Temime S, Daoud D, Gutierrez F, Zarrouk M. 2005. Natural antioxidant composition as correlated to stability of some Tunisian virgin olive oils. Rivista Italiana delle Sostanze Grasse 82, 12-18.
Abdelhamid S, Kamoun G N, Francesco M, Tiziano C. 2013.Genetic similarity among Tunisian cultivated olive estimated through SSR markers. Scientia Agricola 70, 33-38.
Angiolillo A, Mencuccini M, Baldoni L. 1999. Olive genetic diversity assessed using amplified fragment length polymorphism. Theoretical and Applied Genetics 98, 411–421.
Baccouri O, Ceerretani L, Bendini A , Caboni MF, Zarrouk M, Pirrone L, Daoud D. 2007. Preliminary chemical characterization of Tunisian mono-varietal virgin olive oils and comparison with Sicilian ones. European Journal of Lipid Science and Technology 109, 1208–1217.
Belaj A , Satovic Z , Rallo L , Trujillo I. 2002. Genetic diversity and relationships in olive (Olea europaea L.) germplasm collections as determined by randomly amplified polymorphic DNA. Theoretical and Applied Genetics 105, 638–644.
Bogani P, Cavalieri D, Petruccelli R, Polsinelli L, Roselli G. 1994. Identification of olive tree cultivars by using random amplified polymorphic DNA. Acta Horticulturaea 356, 98-101.
Busconi M, Sebastiani L, Fogher C. 2006. Development of SCAR markers for germplasm characterisation in olive tree (Olea europaea L). Molecular Breeding 17, 59-68.
De la Rosa R, Angiolillo A, Guerrero C, Pellegrini M, Rallo L, Besnard G, Berville A, Martin A, Baldoni L. 2003. A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers. Theoretical and Applied Genetics 106, 1273–1282.
Dice LR. 1945. Measures of the amount of ecologic association between species. Ecology 26, 297-302.
Fabbri A, Hormaza JI, Polito VS. 1995. Random amplified polymorphic DNA analysis of olive (Olea europaea L.).Journal of the American Society for Horticultural Science 120, 538–542.
Hess J, Kadereit JW, Vargas P. 2000. The colonization history of Olea europaea L. in Macaronesia based on internal transcribed spacer (RAPD), and (ISSR). Molecular Ecology 9, 857–868.
Gemas VJ, Almadanim MC, Tenreiro R, Martins A, Fevereiro P. 2004. Genetic diversity in the Olive tree (Olea europaea L. subsp. europaea) cultivated in Portugal revealed by RAPD and ISSR markers. Genetic Resources and Crop Evolution 51, 501–511
Kamoun GN, Khlif M, Hamdi M. 1999. Evolution of oils characteristics with maturity of olives in Sfax : Chemlali variety. Acta Horticulturae 474, 701–704.
Kamoun Garrati N, Ayadi M, Khlif M, Trigui A, Karray B, Rekik H, Rekik B, Hamdi T, Arous MN. 2000. Pomological and chemical characterization of Tunisian olive tree (Olea europaea L.). Proceedings of the 4th International olive growing Symposium, CIHEAM-IAM, Barie, Italia, September 25–30, 2000, No. 1–23.
Kamoun Garrati N, Lamy FM, Rebaî A, Gargouri A, Panaud O, Saar A. 2006. Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genetic Resources and Crop Evolution 53, 265–275.
Koutsaftakis A, Koutsifaki F, Stefanoudaki E. 2000. Characterization of extra virgin olive oils obtained from the Koroneiki. Influence of the origin site on several chemical parameters. Olivae 81, 20– 25.
Mataix J, Barbancho FJ. 2006. Olive oil in the Mediterranean food. In: Quiles JL, Ramirez-Tortosa MC, Yaqoob P (eds) Olive oil and health. CABI publishing, Cambridge.
Mehri H, Msallem M, Kamoun MR. 1997. Identification of the principal olive cultivars in Tunisia. Plant. Genetic. Resources. Newsletter 112, 68–72.
Mehri H, Mehri KR. 2007. The Bioagronomic Characterization of a Local Olive Cultivar Gerboui. American Journal of Plant Physiology 2(1), 1-16.
Mnasri Rahmani S, Saddoud Dabbebi O, Ferchichi A. 2013. The study of olive oil quality and morphological biodiversity of Olea europaea L. in the region of “Hbebsa”. Journal of Biodiversity and Environmental Sciences 4, 59-66.
Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of endonucleases. Proceedings of the National Academy Sciences of the United States of America 76, 5269–5273.
Poljuha D, Sladonja B, Setic E, Milotic A, Bandelj D, Jakse J, Javornik B. 2008. DNA fingerprinting of olive varieties in Istria (Croatia) by microsatellite markers. Scientia Horticulturae 115, 223–230.
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding Journal 2, 225–238.
Reale S, Doveri S, Dıaz A, Angiolillo A, Lucentini L, Pilla F, Martin A, Donini P, Lee D. 2006. SNP-based markers for discriminating olive (Olea europaea L.) cultivars. Genome 49, 1193-1205.
Rohlf, M. 1998. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System: Version 2.02. Exeter Software, Setauket, NY, USA.
Taamalli W, Geuna F, Banfi R, BassiD, Daoud D, Zarrouk M. 2006. Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections. Electronic Journal of Biotechnology 9, 467–481.
Trigui A, Msallem M. 2002. Olive of Tunisia, Tunisian Catalogue of Olive Varieties, V1, IRESA, Tunisian Olive Institute.
Visioli F, Poli A, Galli C. 2002. Antioxidant and other biological activities of phenols from olives and olive oil. Medicinal Research Reviews 221, 65–75.
Vos P, Hogers R, Bleeker M, Rejans M, Van De Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.
Mnasri Rahmani Sameh, Saddoud Debbabi Olfa, Ferchichi Ali (2013), AFLP marker-based identification and genetic relationships of olive cultivars in the region of Hbebsa “North West of Tunisia”; JBES, V3, N8, August, P36-41
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