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Research Paper | August 1, 2013

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AFLP marker-based identification and genetic relationships of olive cultivars in the region of Hbebsa “North West of Tunisia”

Mnasri Rahmani Sameh, Saddoud Debbabi Olfa, Ferchichi Ali

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J. Bio. Env. Sci.3(8), 36-41, August 2013

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Abstract

In the region of Hbebsa, little is known about the olive germplasm, and even though there is an important olive biodiversity, studies about characterization and evaluation are scarce. The aim of this work was to make a molecular characterization by the use of amplified fragment length polymorphism (AFLP) markers. A total of 13 olive varieties were genotyped using different EcoRI–MseI AFLP primer combinations. Auto radiographs revealed 92 polymorphic markers in a total of 237 detected fragments. A set of redundant marker patterns was identified and deleted from the binary data matrix; data analysis demonstrated a high degree of polymorphism with an average of 35%. The analysis of AFLP profiles found in our set of olive cultivars showed a wide genetic diversity among olive germplasm. The UPGMA cluster analyses using Jaccard’s index and the Principal coordinate analysis (PCO) revealed that the genetic diversity was predominantly structured according to fruit size. The data obtained can be used for the varietal survey and construction of a database of olive varieties grown in the region of Hbebsa and providing also additional information that could form the basis for the national design of breeding programs.

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AFLP marker-based identification and genetic relationships of olive cultivars in the region of Hbebsa “North West of Tunisia”

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