Paper Details

Agrobacterium mediated production of transgenic tomato plants harbouring AP24 gene from tobacco

Hamidreza Ganjali, Mehdi Dadmehr, Behnaz Korouzhdehi, Mahla Ganjali

DOI: https://dx.doi.org/10.12692/ijb/4.12.263-268

Int. J. Biosci. 4(12), 263-268. June, 2014. (PDF)

Abstract:

The tobacco (Nicotiana tabacum) antifungal AP24 protein identifies as osmotin encodes by its corresponding gene and contributes to pathogen resistance in plants.In the present study the AP24 gene was obtained from tobacco genome by PCR amplification. Following of this successful amplification, transgenic tomato (Solanum lycopersicum) which constitutively expressing the AP24 gene was generated by Agrobacterium tumefaciens mediated transformation. In order to express AP24 protein in tomato, the gene was inserted into a plant expression vector PBI121 and the recombinant pBI-AP24 plasmid was proliferated in E.Coli TG1. The new construct was used to transform the Strain Agrobacterium LBA4404 before plant transformation. Then transgenic tomato plants were developed by introducing AP24 gene in the plant genome under the control of CaMV35s promoter. In order to show the stability of AP24, transgenic T1 tomatoes analyzed by PCR and high transformation proficiencies obtained. Releasing this one new transgenic variety may be a considerable progress toward release varieties enables to show resistant antifungal characteristics.