Alternative vegetable nutrient source for microbial growth

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Research Paper 01/05/2012
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Alternative vegetable nutrient source for microbial growth

Deivanayaki M, Antony Iruthayaraj P
Int. J. Biosci.2( 5), 47-51, May 2012.
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The high cost of the microbial culture media paved a way for the production of alternative media using cheap local raw materials (Potato, Groundnut, Cereals, Cassava, Yam, Pigeon pea, Maize and Beans). The vegetables being a good source of nutrients are used to formulate both solid and liquid media for the growth of selected bacteria and fungi. Their growth on the formulated media was compared with growth on the conventional media. All the formulations produced good growth of microbes similar to the conventional media. In the solid media formulation, Staphylococcus sp., produced 230 CFU / ml in Formulation E, 150 CFU / ml of Escherichia coli in Formulation A, 170 CFU / ml of Salmonella sp., in Formulation B, 150 CFU / ml of Klebsiella sp., in Formulation B, 180 cells / 0.1 ml of Aspergillus sp., in Formulation A and 150 cells / 0.1 ml of yeast in Formulation C. In broth formulations, Staphylococcus sp., Escherichia coli, Klebsiella sp., and Salmonella sp., produced 250, 200, 150, 175 cells / 0.1 ml of sample in theFormulations I, G, H and J respectively. Comparing with the performance on conventional bacteriological and mycological media, the prepared vegetable media is found to be a good and cheap media material for the isolation and cultivation of both bacteria and fungi.


Adesemoye AO, Adedire CO. 2005. Use of Cereals as basal medium for the formulation of alternative culture media for fungi. World Journal of Microbiology & Biotechnology 21, 329-336.

Famurewa O, David OM. 2008. Formulation and Evaluation of Dehydrated Microbiological Media from Avocado Pea (Peasea Americana Cmill). Research Journal of Microbiology 3(5), 326-330.

John Lindquist. 2006. General Microbiology – A lab manual. 4th Edition, Mc Graw – Hill Primes custom publishing.

Kuria P, Demo P, Nyende AB, Kahangi EM. 2008. Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of Potato (Solanum tuberosum L.). African Journal of Biotechnology 7(3), 301-307.

Laleye SA. 1990. Modification of Potato extract medium for the growth of some selected organisms (Unpublished B.Sc., Thesis, Ado-Ekiti).

Laleye SA, Tedela PO, Adesua B, Famurewa O. 2007. Growth of Some Microorganisms on Media Formulated from Local Raw Materials. Research Journal of Microbiology 2(6), 545-549.

Madigan MT, Martinko, Parker J. 2000. Nutrition and Metabolism. In:Brock Biology of Microorganisms. 9th Edition, Prentice Hall, New Jersey, 102-134.

Pelczar JM, Chan LEA, Krieg NR. 1993. Microbiology, Concept and Application. International Edition, McGraw-Hill Inc. New Jersy, 847.

Tharmila S, Jeyaseelan EC, Thavaranjit AC. 2011. Preliminary screening of alternative culture media for the growth of some selected fungi. Archives of Applied Science Research 3(3), 389-393.

Seddon SV, Boriello SP. 1989. A Chemically defined minimal medium for Clostridium difficile. Letters in Applied Microbiology 9, 237-239.

Simin Hagh Nazari. 2011. Sonicated date syrup media preparation for microbial culture. African Journal of Biotechnology 10(3), 424-432.

Umechuruba CF, Elenwo EN. 1999. Diagnostic Techniques in Mycology. Beek Publishers, Port-Hercout. 30 – 56.