Welcome to International Network for Natural Sciences | INNSpub

Amperometric Glucose Biosensor Based on Activated Carbon/5-Methyl 1, HydroxyPhenazine/Glucose Oxidase Matrix

Research Paper | December 1, 2016

| Download 4

Sameen Yousaf, Maira Anam, Naeem Ali

Key Words:

Int. J. Biosci.9( 6), 193-203, December 2016

DOI: http://dx.doi.org/10.12692/ijb/9.6.193-203


IJB 2016 [Generate Certificate]


Recently, advance bio-sensing devices have given considerable importance, because of their capability to identify the target compounds promptly. In this context,2nd generation glucose biosensor has been fabricated using natural redox compound, 5-methyl 1, hydroxyphenazine (5-MHP)obtained from P. aeruginosa. Amperometric detection was based on interaction of glucose with the working electrode loaded with activated carbon (AC), 5-MHP and glucose oxidase (GOx), AC/5-MHP/GOx. Working electrodes prepared at three different temperatures 4oC, 25oC and 47oC, sensitively detect glucose andshowed the linear ranges of R2 = 0.98, R2 = 0.98 and R2 = 0.99 respectively with detection limit of 0.3µM at signal-to-noise ratio (S/N)=3. It was found that GOx immobilization temperature directly influence the long term efficiency of glucose biosensor. Electrode fabricated at 4oC exhibited greater operational stability i.e. 74% followed by 25oC (68%) and 47oC (48%). Furthermore, theresponse timewith eachglucoseconcentration (2.0 to 26.0mM) was relatively less i.e. 2s, for enzyme electrode fabricated at 4oC whereas it was 4s and 5s for working electrodes prepared at 25oC and 47oC respectively.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Amperometric Glucose Biosensor Based on Activated Carbon/5-Methyl 1, HydroxyPhenazine/Glucose Oxidase Matrix

Ali N, Yousaf S, Anam M, Bangash Z, Maleeha S. 2016. Evaluating the efficiency of a mixed culture biofilm for the treatment of black liquor and molasses in a mediator-less microbial fuel cell. Environmental Technology 37(22), 2815-2822. http://dx.doi.org/10.1080/09593330.2016.1166267

Beckmann S, Welte C, Li X, Oo YM, Kroeninger L, Heo Y, Zhang M, Ribeiro D, Lee M, Bhadbhade M. 2016. Novel phenazine crystals enable direct electron transfer to methanogens in anaerobic digestion by redox potential modulation. Energy & Environmental Science 9(2), 644-655. http://dx.doi.org/10.1039/C5EE03085D

Bossi A, Piletsky SA, Piletska EV, Righetti PG, Turner AP. 2000. An assay for ascorbic acid based on polyaniline-coated microplates. Analytical chemistry 72(18), 4296-4300. http://dx.doi.org/10.1021/ac000185s

Chen C, Xie Q, Yang D, Xiao H, Fu Y, Tan Y, Yao S. 2013. Recent advances in electrochemical glucose biosensors: a review. Royal Society of Chemistry Advances 3(14), 4473-4491. http://dx.doi.org./10.1039/C2RA22351A

Chen W, Liu XY, Qan C, Song XN, Li WW, Yu HQ. 2015. An UV–vis spectroelectrochemical approach for rapid detection of phenazines and exploration of their redox characteristics. Biosensors and Bioelectronics 64, 25-29. http://dx.doi.org/10.1016/j.bios.2014.08.032

Cheng H, Xu L, Zhang H, Yu A, Lai G. 2016. Enzymatically catalytic signal tracing by a glucose oxidase and ferrocene dually functionalized nanoporous gold nanoprobe for ultrasensitive electrochemical measurement of a tumor biomarker. Analyst 141(14), 4381-4387. http://.dx.doi.org/10.1039/C6AN00651E

Devasenathipathy R, Mani V, Chen S-M, Huang S-T, Huang T-T, Lin C-M, Hwa K-Y, Chen T-Y, and Chen B-J. 2015. Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes. Enzyme and Microbial Technology 78, 40-45. http://dx.doi.org/10.1016/j.enzmictec.2015.06.006

Dong S, Yang Q, Peng L, Fang Y, Huang T. 2016. Dendritic Ag@ Cu bimetallic interface for enhanced electrochemical responses on glucose and hydrogen peroxide. Sensors and Actuators B: Chemical 232, 375-382. http://dx.doi.org/10.1016/j.snb.2016.03.129

Hartmann M, Kostrov X. 2013. Immobilization of enzymes on porous silicas–benefits and challenges. Chemical Society Reviews 42(15), 6277-6289. http://.dx.doi.org/10.1039/C3CS60021A

Hossain MF, Park JY. 2016. Plain to point network reduced graphene oxide – activated carbon composites decorated with platinum nanoparticles for urine glucose detection. Scientific Reports 6, 1-10. http://dx.doi.org./10.1038/srep21009

Jain A, Ong V, Jayaraman S, Balasubramanian R, Srinivasan M. 2016. Supercritical fluid immobilization of horseradish peroxidase on high surface area mesoporous activated carbon. The Journal of Supercritical Fluids 107, 513-518. http://dx.doi.org/10.1016/j.supflu.2015.06.026

Katz E, Fernández VM, Pita M. 2015. Switchable bioelectrocatalysis controlled by pH changes. Electroanalysis 27(9), 2063-2073. http://dx.doi.org./10.1002/elan.201500211

Kavosi B, Hallaj R, Teymourian H, Salimi A. 2014. Au nanoparticles/PAMAM dendrimer functionalized wired ethyleneamine–viologen as highly efficient interface for ultra-sensitive α-fetoprotein electrochemical immunosensor. Biosensors and Bioelectronics 59, 389-396 http://dx.doi.org/10.1016/j.bios.2014.03.049

Kennedy RK, Naik PR, Veena V, Lakshmi B, Lakshmi P, Krishna R, Sakthivel N. 2015. 5-Methyl phenazine-1-carboxylic acid: a novel bioactive metabolite by a rhizosphere soil bacterium that exhibits potent antimicrobial and anticancer activities. Chemico-Biological interactions 231, 71-82. http://dx.doi.org/10.1016/j.cbi.2015.03.002

Kubendhiran S, Sakthinathan S, Chen S-M, Lee CM, Lou B-S, Sireesha P, Su C. 2016. Electrochemically Activated Screen Printed Carbon Electrode Decorated with Nickel Nano Particles for the Detection of Glucose in Human Serum and Human Urine Sample. International Journal of Electrochemical Sciences11, 7934-7946. http://dx.doi.org./10.20964/2016.09.11

Liu H, Ying T, Sun K, Li H, Qi D. 1997. Reagentless amperometric biosensors highly sensitive to hydrogen peroxide, glucose and lactose based on N-methyl phenazine methosulfate incorporated in a Nafion film as an electron transfer mediator between horseradish peroxidase and an electrode. Analytica Chimica Acta 344(3), 187-199. http://dx.doi.org/10.1016/S00032670(97)00047-0

Liu Y, Zhang X, He D, Ma F, Fu Q, Hu Y. 2016. An amperometric glucose biosensor based on a MnO 2/graphene composite modified electrode. Royal Society of Chemistry Advances 6(22), 18654-18661. http://dx.doi.org./10.1039/C6RA02680J

Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K. 2006. Microbial fuel cells: methodology and technology. Environmental science & technology 40(17), 5181-5192. http://dx.doi.org./10.1021/es0605016

Mazzola PG, Lopes AM, Hasmann FA, Jozala AF, Penna TC, Magalhaes PO, RangelYagui CO, Pessoa Jr A. 2008. Liquid–liquid extraction of biomolecules: an overview and update of the main techniques. Journal of Chemical Technology and Biotechnology 83(2), 143-157. http://dx.doi.org./10.1002/jctb.1794

Morthensen ST, Meyer AS, Jørgensen H, Pinelo M. 2017. Significance of membrane bioreactor design on the biocatalytic performance of glucose oxidase and catalase: Free vs. immobilized enzyme systems. Biochemical Engineering Journal 117, 41-47. http://dx.doi.org/10.1016/j.bej.2016.09.015

Pandey P, Panday D. 2016. Tetrahydrofuran and hydrogen peroxide mediated conversion of potassium hexacyanoferrate into Prussian blue nanoparticles: Application to hydrogen peroxide sensing. Electrochimica Acta 190, 758-765. http://dx.doi.org/10.1016/j.electacta.2015.12.188

Rueda N, dos Santos J, Ortiz C, Torres R, Barbosa O, Rodrigues RC, BerenguerMurcia Á, FernandezLafuente R. 2016. Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities. The Chemical Record 16(3), 1436–1455. http://dx.doi.org./10.1002/tcr.201600007

Salvador J, Marco M. 2016. Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water. Electroanalysis 28(8), 1833–1838. http://dx.doi.org./10.1002/elan.201600172

Su Z. 2016. New activated carbon with high thermal conductivity and its microwave regeneration performance. Journal of Wuhan University of Technology-Matererial Science Edition31(2), 328-333. http://dx.doi.org./10.1007/s11595-016-1371-2

Tang M, Lin X, Li M, Li J, Ni L, Yin S. 2016. Construction of amperometric glucose biosensor based on in-situ fabricated hierarchical meso-macroporous SiO2 modified Au film electrodes. Journal of Wuhan University of Technology-Material  Science Edition 31(4), 736-742. http://dx.doi.org./10.1007/s11595-016-1439-z

Vostiar I, Tkac J, Sturdik E, Gemeiner P. 2002. Amperometric urea biosensor based on urease and electropolymerized toluidine blue dye as a pH-sensitive redox probe. Bioelectrochemistry 56(1), 113-115. http://dx.doi.org/10.1016/S1567-5394(02)00042-7

Yu Y, Chen Z, He S, Zhang B, Li X, Yao M. 2014. Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode. Biosensors and Bioelectronics 52, 147-152. http://dx.doi.org/10.1016/j.bios.2013.08.043

Zhang T, Zhang L, Su W, Gao P, Li D, He X, Zhang Y. 2011. The direct electrocatalysis of phenazine-1-carboxylic acid excreted by Pseudomonas alcaliphila under alkaline condition in microbial fuel cells. Bioresource technology 102(14), 7099-7102. http://dx.doi.org/10.1016/j.biortech.2011.04.093

Zhu Z, Garcia-Gancedo L, Flewitt AJ, Xie H, Moussy F, Milne WI. 2012. A critical review of glucose biosensors based on carbon nanomaterials: carbon nanotubes and graphene. Sensors 12(5), 5996-6022. http://dx.doi.org./http://dx.doi.org/10.3390/s120505996