An antifungal phenazine pigment obtained from Pseudomonas aeruginosa inhibits the growth of Aspergillus ochraceus

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An antifungal phenazine pigment obtained from Pseudomonas aeruginosa inhibits the growth of Aspergillus ochraceus

Popy Devnath, Md. Kamal Uddin, Md. Faysal Kamal, Md. Towhid Hossain, Mohammed Abul Manchur
Int. J. Biosci.17( 2), 140-148, August 2020.
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Aspergillus ochraceus, a pathogenic mold frequently found in grains, soil, and dried food products. This mold can produce several toxins especially Ochratoxin A and B on infected crops. These toxins are associated with food intoxications both in humans and animals. This study aimed to evaluate the antifungal potentiality of a pigment produced by Pseudomonas aeruginosa, against A. ochraceus in-vitro. Primarily, the cross streak method showed P. aeruginosa (isolate PU8 and PU10) inhibits the growth of A. ochraceus. Then in the quantitative assay using crude extract of the isolate showed significant inhibitory (p<0.05) activities which were up to 72% inhibition by PU8 and 59% by PU10. Then the pigment was extracted, purified, and characterized. The UV spectrophotometry, FT-IR (Fourier Transform Infrared Spectrometry), TLC (Thin-Layer Chromatography) and microscopic analysis proved that the crude extract contained pyocyanin as a potent antifungal phenazine pigment. The adverse effects of chemical fungicides necessitated the use of eco-friendly biological control agents against fungi. Fortunately, from this study, we can infer that P. aeruginosa can produce an antifungal phenazine pigment pyocyanin that inhibits the growth of A. ochraceus and be used as a potential bio-control agent.


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