An exposure study of endocrine disruptors on haematology of fresh water fish Labeo rohita

Paper Details

Research Paper 01/02/2018
Views (315) Download (24)

An exposure study of endocrine disruptors on haematology of fresh water fish Labeo rohita

Misbah Rashid, Ghazala Jabeen, Sofia Nosheen
Int. J. Biosci.12( 2), 136-140, February 2018.
Certificate: IJB 2018 [Generate Certificate]


Synthetic chemicals released in water bodies resulting in exposure to fresh water organisms and enter in food chain. Phthalates and parabens are those chemicals now being studied for their endocrine disrupting potentials. This study was designed to determine the haematological effects of phthalate, parabens and their mixtures on fresh water and commonly eaten fish Labeo rohita. To healthy fish (Labeo rohita) of juvenile age, experimental dose of ester of phthalate, paraben and their mixture were given as treatment group for 30 days. Haematological parameters were studied. A significant fluctuation was seen in erythrocytes (0.025+0.005), packed cell volume (0.15+0.05), haemoglobin (0.3+0.1*), platelets (12 + 2*), mean corpuscular haemoglobin (726.5+623.5), and mean corpuscular haemoglobin concentration (7050+6950) as compared to control. Dimethyl phthalate (DMP) and dibutyl phthalate (DBP) treated fish showed a statically significant decrease in erythrocytes (0.025+0.005*; 0.08+0.07*) and platelets (12 + 2*; 71+1.5*) as compared to fish of other groups. Fluctuations in blood parameters may lead to further abnormalities in an organism.


Ahmad Y, Andrabi SM, Hussain A, Hussain G, Wani AR, Bannetwala RC, Tharani M. 2015. Haematological studies of fresh water fish, Labeo rohita (Ham.) exposed to heavy metals. International Journal of Science Environment and Technology 4(6), 1513 – 1523.

Ahmed Z. 2011. Acute toxicity and haematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. African Journal of Biotechnology 10(63), 13852-13859.

Akinrotimi O, Agokei EO, Aranyo AA. 2012. Changes in blood parameters of Tilapia guineensis exposed to different salinity levels. Journal of Environmental Engineering and Technology 1, 4-12.

Boberg J, Taxvig C, Christiansen S, Hass U. 2010. Possible endocrine disrupting effects of parabens and their metabolites. Reproductive Toxicology 30(2), 301-12.

Dacie JV, Lewis SM. 2001. Practical haematology, 6th Edn. ELBS and Churchill, Livingston comp clinpathol.

Darbre PD, Harvey PW. 2008. Paraben esters: review of recent studies of endocrine toxicity, absorption, esterase and human exposure, and discussion of potential human health risks. Journal of Applied Toxicology 28(5), 561-78.

Di Giullio RT, Hinton DE. 2008. The Toxicology of Fishes. Taylor & Francis; 1071.

Frenzilli G, Nigro M, Lyons BP. 2009. The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutation Research 681, 80-92.

Hoberman AM, Schreur DK, Leazer T, Daston GP, Carthew P, Re T, Loretz L, Mann P. 2008. Lack of effect of butylparaben and methylparaben on the reproductive system in male rats. Birth Defects Research B, Developmental and Reproductive Toxicology 83(2), 123-33.

Koeppe ES, Ferguson KK, Colacino JA,Meeker JD. 2013. Relationship between urinary triclosan and paraben concentrations and serum thyroid measures in NHANES, 2007-8. Science of the Total Environment 445-6, 299–305.

Kori-Siakpere O, Oboh EC. 2011. Haematological effects of sublethal concentrations of tobacco leaf dust on the African catfish: Clarias gariepinus (Burchell 1822) Archives of Applied Science Research 3(2), 493-502.

Kumar N, Prabhu PAJ, Pal AK, Remya S, Aklakur M, Rana RS, Gupta S, Raman S,Jadhao B. 2011. Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pesticide Biochemistry and Physiology 99, 45–52.

Latif A, Khalid M, Ali M. 2014. An assessment of naphthalene stress on renal and hepatic functional integrity in Labeo rohita. International Journal of Current Engineering and Technology 4(1), 319-324.

Latif A, Khalid M, Ali M. 2014. Evaluation of toxic stress of copper sulphate and lead nitrate on hematological and serum biochemical characteristics of freshwater Cyprinid (Labeo rohita). International Journal of Current Engineering and Technology 4(1), 366-372.

Meeker JD, Yang T, Ye X, Calafat AM, Hauser R. 2011. Urinary concentrations of parabens and serum hormone levels, semen quality parameters, and sperm DNA damage. Environmental Health Perspectives 119(2), 252–257.

Oishi S. 2002a. Effects of propyl paraben on the male reproductive system. Food and Chemical Toxicology 40(12), 1807-13.

Oishi S. 2002b. Effects of butyl paraben on the male reproductive system in mice. Archives Toxicology 76(7), 423-9.

Sepperumal U, Saminathan S. 2013. Effect of diethyl phthalate on the haematological parameters of the freshwater fish Oreochromis mossambicus (Tilapia). European Journal of Zoological Research 2(4), 55–59.

Sumpter JP. 2009. Protecting aquatic organisms from chemicals: the harsh realities. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences 367, 3877-3894.