An investigation of tissue culture and co-cultures of different explants in Calendula officinalis

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Research Paper 01/12/2013
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An investigation of tissue culture and co-cultures of different explants in Calendula officinalis

Ali Ashraf Mehrabi, Ehsan Khodadadi, Zeinab Sadeghi, Lia shooshtari
Int. J. Biosci.3( 12), 201-205, December 2013.
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Two experiments were conducted separately to investigate the effects of the medium and explant types on the callus induction and regeneration of Calendula officinalisas a medicinal plant. In the first experiment, explants were placed on a ¼ MS medium on three levels of NAA (1-Naphthaleneacetic acid) and KIN (Kinetin) in sterile conditions. The parameters, time to callus initiation, callus volume, and the percentage of callus induction were measured to evaluate the callus condition in this experiment. Variance analysis of the results on the first experiment showed that there was no considerable difference in terms of callus induction in mediums with a combination of hormones, while the effect of explant source on these traits was significant. In the second experiment (co-culturing of different explants), main effects of cotyledon, hypocotyl and meristem explants as single cultures, and also effects of cotyledon and meristem, hypocotyl and meristem explants as co-cultures in the two mediums, KIN + NAA and TDZ (Thidiazuron) + IBA (Indole-3-butyric acid), were studied. The results of the second experiment showed that co-cultured explants have a better regeneration. It also indicated a better condition for traits related to the root of the co-culture system. Moreover, the impact of the two explants, hypocotyland meristem, together onrootingandregenerationwas remarkable.


Anna G. Wirginig J. 2002. Initation and growth characteristics of suspension cultures of Calendula Officinalis cell. Plant cell. Tissue and organ culture 71, 29-40.

Bernath J. 2000. medicinal and aromatic plants. Mezzo. Pabl. Budapest, 667.

Bilia AR, Bergonzi MS, Gallori S, Mazzi G, Vincieri FF. 2002. Stability of the constituents of Calendula Officinalis milk thistle and passion flower tinetures, journal of pharmaceutical and biomedical analysis 30, 613-624.

Coco S, Uranbey A, Pek KM, Khawar EO, Sariham MD, Kaya IP, Armakisiz S. 2004. Adventitious shoot regeneration and micro propagation in Calendula Officinalis biologia, Plantarum 43, 499-451.

Duke JA. 1985. handbook of medicinal herbs crop ,press Bocan raton, florida.

Grezelak A, Wirnia J. 2002. Initiation and growth characteristics of suspension cultures of Calendula  Officinalis cell, Plant Cell, Tissue and Organ Culture 71, 29–40, 2002 71, 29-40.

Martin F. 2005. a growers manual for calendula officinalis

Paploe V, Andres C, Maelena V, Octavio PL. 2002. Plant regeneration via organogenesis in Calendula officinalis. Plant cell, tissue and organ cultuve 69, 279-283.

Pout GR, Samantaras DA. 2000. In vitro manipulation and propagation of medicinal plnats biotechnology advances 18, 91-120.

Ramachandra SR, Rauishankar GA. 2002. Chemical factories of secondary metabolites. Plant cell cultures, Biotechnology advance 20, 101-153.

Tripathi L. 2003. Role of biotechnology in medicinal plants. Tropical journal of pharmaceutical research 22, 243-253.