An investigation on heavy metal tolerance properties of bacteria isolated from textile effluent

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Research Paper 01/12/2015
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An investigation on heavy metal tolerance properties of bacteria isolated from textile effluent

Md. Ashikuzzaman, Sayeed Shahriyar, Mohammed Bakhtiar Lijon, Md. Atiqur Rahman, Md. Mahedi Hassan, Abdulla-Al-Asif
J. Bio. Env. Sci.7( 6), 62-71, December 2015.
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The presence of high concentration of toxic heavy metals in industrial waste directly leads to contamination of receiving soil and water bodies and has deleterious impact on both human health and aquatic life. In the present study samples from textile mill effluent from different areas of Jessore city were analyzed for the identification and characterization of bacteria which shows tolerance to Copper, Mercury and Zinc. The bacterial isolates were characterized on the basis of their morphological and physiological studies including size and shape of the organisms, arrangement of the cells, presence or absence of spores, regular or irregular forms, gram reaction, cultural characteristics, IMViC test, H2S production, nitrate reduction, deep glucose agar test etc. All the bacterial isolates belonged to 3 genera Bacillus, Enterobacter and Pseudomonas. All the gram positive isolates used in our study showed highest level of tolerance to Zn and moderate level of tolerance to Cu while gram negative isolates showed higher tolerance to Zn in comparison with Cu in nutrient broth. But all of the isolates showed almost no tolerance to Hg. So, our bacterial isolates have the probability to use in the treatment of industrial effluent containing heavy metals and thus pollution due to heavy metal can be controlled. The goal of this study was to identify heavy metal tolerant bacteria from the textile effluent. This kind of study is very significant for broader investigation to obtain data about metal tolerant bacteria considering their potential use for bioremediation and about the interactions between metals and bacteria.


Basu M, Bhattacharya S, Paul AK. 1997. Isolation and characterization of chromium-resistant bacteria from tannery effluents. Bulletin of Environmental Contamination and Toxicology 58, 535-542.

Castro-Silva MA, Souza Lima, AO, Gerchenski AV, Jaques DB, Rodrigues AL, Lima de Souza P, Rörig LR. 2003. Heavy metal resistance of microorganisms isolated from coal mining environments of Santa Catarina. Brazillian Journal of Microbiology 34, 45-47.

Choudhury P, Kumar R. 1998. Multidrug and metal-resistant strains of Klebsiellapneumoniae isolated from Penaeusmonodon of the coastal waters of deltaic Sundarban. Canadian Journal of Microbiology 44, 186-189.

De J, Ramaiah N, Mesquita A, Verlekar XN. 2003. Tolerance to various toxicants by marine bacteria highly resistant to mercury. Marine Biotechnology 5, 185-193.

Durve A, Naphade S, Bhot M, Varghese J, Chandra N. 2012. Characterisation of metal and xenobiotic resistance in bacteria isolated from textile effluent. Pelagia Research Library Advances in Applied Science Research 3, 2801-2806.

Duxbury T. 1986. Microbes and heavy metals: an ecological overview. Microbiology Science 3, 330-333.

Eccles H. 1995. Removal of heavy metals from effluents streams- Why select a biological process? International Biodeterioration & Biodegradation 35, 5–16.

Fagade OE, Adetutu EM. 1999. Lead solubilization and accumulation by two strains of Pseudomonas species obtained from a battery manufacturing factory effluent. Nigeria Journal of Miccrobiology 13, 39-46.

Faisal M, Hasnain S. 2004. Microbial conversion of Cr (VI) in to Cr (III) in industrial effluent. African Journal Biotechnology 3, 610-617.

Garbarino JR, Hayes H, Roth D. 1995. Contaminants in the Mississippi river. U. S. Geological Survey Circular, Virginia, U.S.A. 1133 p.

Haefeli C, Franklin C, Hardy K. 1984. Plasmid-determined silver resistance in Pseudomonas stutzeri isolated from a silver mine. Journal of Bacteriology 158: 389-392.

Kumar RA, Moharikar A, Purohit HJ. 2005. Microbial population dynamics at effluent treatment plants. Journal of Environmental Monitoring 7, 552-558.

Lima e Silva AA. 2012. Heavy metal tolerance (Cr, Ag AND Hg) in bacteria isolated from sewage. Brazilian Journal of Microbiology: 1620-1631.

Malik A. 2004. Metal bioremediation through growing cells. Environmental International 30, 261-278.

Mindlin S, Kholodii G, Gorlenko Z, Minakhina S, Minakhin L, Kalyaeva E, Kopteva A, Petrova M, Yurieva O, Nikiforov V. 2001. Mercury resistance transposons of Gram-negative environmental bacteria and their classification. Research in Microbiology 152, 811-822.

Nese T, Sivri N, Toroz I. 2007. Pollutants of Textile Industry Wastewater an Assessment of its Discharge Limits by Water Quality Standards. Turkish Journal of Fisheries and Aquatic Sciences 7, 97-103.

Olukoya DK, Smith SI, Ilori MO. 1997. Isolation and characterization of heavy metals resistant bacteria from Lagos Lagoon. Folia Microbiol (Praha). 42, 441-444.

Oyetibo GO, Ilori MO, Adebusoye SA, Obayori OS, Amund OO. 2010. Bacteria with dual resistance to elevated concentrations of heavy metals and antibiotics in Nigeria in contaminated systems. Environmental Monitoring Assessment 168, 305-314.

Raghukumar C, DeSouza DT, Tiwari R, Sah AK. 2006. Enhanced production of laccase by a marine fungus during treatment of coloured effluents and synthetic dyes. Enzyme and Microbial Technology 38, 504-511.

Silver S, Misra TK. 1988. Plasmid-mediated heavy metal resistances. Annual Review of Microbiology 42, 717-743.

Sobolev D, Begonia MF. 2008. Effects of heavy metal contamination upon soil microbes: lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers. International Journal of Environmental Research and Public Health 5, 450-456.

Wang  C,  Yediler  A,  Lienert  D,  Wang  Z, Kettrup A. 2002.  Toxicity  evaluation  of  reactive dyestuffs, auxiliaries and selected effluents in textile finishing  industry  to  luminescent  bacteria  Vibrio fischeri. Chemosphere 46, 339-344.