Analysis of genetic diversity in bindweed (Convolvulus arvensis L.) populations using random amplified polymorphic DNA (RAPD) markers
Paper Details
Analysis of genetic diversity in bindweed (Convolvulus arvensis L.) populations using random amplified polymorphic DNA (RAPD) markers
Abstract
Bindweed (Convolvulus arvensis L.) is one of the most harmful weed species in Turkey. Using random amplified polymorphic DNA (RAPD) markers, It investigated the genetic diversity within and among five local populations of bindweed. Fourteen (14) RAPD primers produced a total of 429 bands, of which 142 (33.1%) were polymorphic. The mean Nei’s gene diversity value for all five populations was 0.2053. Shannon’s information index varied with a population (0.2278–0.3082), averaging 0.3047. Analysis of molecular variance (AMOVA) presented 53.8% variation within populations, and 32.9% among populations, showing a high variation within populations. Additionally, the variation between groups was 13.3%. The genetic differentiation among populations (GST) was 0.293, indicating that most genetic diversity occurs within populations. Gene flow (Nm) was low, at only 0.6032.
Austin DF. 2000. Bindweed (Convolvulus arvensis, Convolvulaceae) in North America: from medicine to menace. Journal of the Torrey Botanical Society 127, 172-177.
Barrett SCH. 1992. Genetics of weed invasions. In: Jain SK, Botsford LW (eds) Applied populationbiology. Kluwer, Dordrecht, 91–119 p.
Coruh I, Zengin H. 2007. Determination of economic threshold of field bindweed (Convolvulus arvensis L.) in spring wheat in Erzurum, Turkey. Journal of Ataturk University Agricultural Faculty 38, 151-157.
Fedorenko OM, Gritskikh MV. 2008. Diversity of Arabidobsis thaliana (L.) Heynh. natural populations at the northern limits of the species range: RAPD analysis. Russian Journal of Genetics 44(4), 425– 428.
Garcia-Baudin JM, Darmency H. 1979. Differences intraspecifiques chez Convolvulus arvensis L. Weed Research 19, 219-224.
Guo HB, Lu BR, Wu QH, Chen JK, Zhou TS. 2007. Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms. Genetic Resources Crop Evoutionl 54, 917-924.
Hamrick JL, Godt MJW. 1989. Allozyme diversity in plant species. In: Brown A.H.D., M.T. Clegg, A.L. Kahler, B.S. Weir (eds) Plant population genetics, breeding and genetic resources. Sinauer Associates, Sunderland 43–46 p.
Holm LG, Plunknett DL, Pancho JV, Herberger JP. 1977. Honolulu, Hawaii, USA: University Press of Hawaii pp. 98–104.
Li W, Wang B, Wang B. 2006. Lack of genetic variation of an invasive clonal plant Eichornia crassipes in China revealed by RAPD and ISSR markers. Aquatic Botany 84, 176–180.
Li G, Quiros CF. 2001. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103, 455–461.
Nianxi Z, Yubao G, Wang J, Ren A, Xu H. 2006. RAPD diversity of Stipa grandis populations and its relationship with some ecological factors. Acta Ecologica Sinica 26(5), 1312–1319.
O’Hanlon PC, Peakal R, Briese DT. 2000. A review of new PCR-based genetic markers and their utility to weed ecology. Weed Research 40, 239-254.
Qian ZQ, Xu L, Wang YL, Yang J, Zhao GF. 2008. Ecological genetics of Reaumuria soongorica Pall. Maxim. population in the oasis-desert ecotone in Fukang, Xinjiang, and its implications for molecular evolution. Biochemical Systematics and Ecology 36, 593–601.
Reisch C, Anke A, Rohl M. 2005. Molecular variation within and between ten populations of Primula farinosa (Primulaceae) along an altitudinal gradient in the northern Alps. Basic and Applied Ecology 6, 35–45.
Schaal BA, Hayworth DA, Olsen KM, Rauscher JT, Smith WA. 1998. Phylogeographic studies in plants: problems and prospects. Molecular Ecology 7, 465–474.
Schroeder D, MiiUer-Schihr H, Stinson CSA. 1993. A European weed surveyin 10 major crop systems to identrfy targets for biological control. Weed Research 33, 449-458.
Sun K, Chen W, Ma RJ, Chen X, Ang L, Ge S. 2006. Genetic variation in Hippophae rhamnoides ssp. sinensis (Elaeagnaceae) revealed by RAPD markers. Biochemical Genetics 44(5–6), 186–197.
Sunar S, Aksakal O, Yildirim N, Agar G, Gulluce M, Sahin F. 2009. Genetic diversity and relationships detected by FAME and RAPD analysis among Thymus species growing in eastern Anatolia region of Turkey. Romanian Biotechnological Letters 14(2), 4313–4318.
Wright S. 1951. The genetical structure of populations. Annals of Eugenics 15, 323–354.
Xia T, Chen SL, Chen SY, Ge XJ. 2005. Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers. Biochemical Genetics 43(3–4), 87–101.
Yeh FC, Yang RC, Boyle T. 1999. Popgene version 1.31. Microsoft windows-based freeware for population genetic analysis. University of Alberta, Canada Biochemical Genetics 48, 603–611.
Vagelgsang S. 1998. Pre-emergence efficiacy of Phomopsis convolvulus Ormeno to control field bindweed (Convolvulsu arvensis L.). Ph. Thesis, Montreal, Canada.
Ward S, Jasieniuk M. 2009. Rewiev: Sampling weedy and invasive plant populations for genetic diversity analysis. Weed Science 57, 593-602.
Serap Sunar, Guleray Agar, Gokce Nardemir (2015), Analysis of genetic diversity in bindweed (Convolvulus arvensis L.) populations using random amplified polymorphic DNA (RAPD) markers; JBES, V7, N1, July, P197-204
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