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Research Paper | July 1, 2015

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Analysis of genetic diversity in bindweed (Convolvulus arvensis L.) populations using random amplified polymorphic DNA (RAPD) markers

Serap Sunar, Guleray Agar, Gokce Nardemir

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J. Bio. Env. Sci.7(1), 197-204, July 2015

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Abstract

Bindweed (Convolvulus arvensis L.) is one of the most harmful weed species in Turkey. Using random amplified polymorphic DNA (RAPD) markers, It investigated the genetic diversity within and among five local populations of bindweed. Fourteen (14) RAPD primers produced a total of 429 bands, of which 142 (33.1%) were polymorphic. The mean Nei’s gene diversity value for all five populations was 0.2053. Shannon’s information index varied with a population (0.2278–0.3082), averaging 0.3047. Analysis of molecular variance (AMOVA) presented 53.8% variation within populations, and 32.9% among populations, showing a high variation within populations. Additionally, the variation between groups was 13.3%. The genetic differentiation among populations (GST) was 0.293, indicating that most genetic diversity occurs within populations. Gene flow (Nm) was low, at only 0.6032.

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Analysis of genetic diversity in bindweed (Convolvulus arvensis L.) populations using random amplified polymorphic DNA (RAPD) markers

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