Analysis segregation and prediction of gene action controlling soybean tolerance to aluminum toxicity

Paper Details

Research Paper 01/10/2014
Views (205) Download (3)

Analysis segregation and prediction of gene action controlling soybean tolerance to aluminum toxicity

Arvita Netti Sihaloho, Trikoesoemaningtyas, Didy Sopandie, Desta Wirnas
J. Bio. Env. Sci.5( 4), 409-418, October 2014.
Certificate: JBES 2014 [Generate Certificate]


This experiment consists of two stage i.e: (1) Study inheritance root re-growth in nutrient culture. The objective of this research was to study soybean inheritance tolerance to aluminum stress and gene action at seedling stage and acid soil. This research used 120 seeds of F2 population generated from crossing between Argomulyo (Al-sensitive) with Tanggamus (Al-tolerant). Seeds were grown in nutrient culture for six days (two days without Al, two days with Al concentration 1.5 mM and pH 4.0 then two days more without Al) to see root length under condition stress and recovery. The result showed root length had high and medium heritability at condition stress and recovery, that means root length is easily be inherited because root length was larger influenced by genetic compared to the environment factors. The root re-growth on F2 population at Argomulyo were 83 plants and Tanggamus were 33 plants. (2) Study inheritance of agronomy characters in the acid soil. Studied inheritance tolerance to Al stress at acid soil showed that number of productive branches, seed weight by plant and 100 seeds weight had moderate heritability so the characters were used as character selection for next generation.


Agriculture Ministry. 2012. Varieties Utilization Status and needs.

Agriculture Ministry. 2012. Varieties Description.

Arsyad DM, Adie MM, Kuswantoro H. 2007. Superior Varieties Of Soybean Specific Assembly Agroekologi. In: Sumarno, Suyamto, Widjono A, Hermanto, Kasim H (eds.). Soybean. Agricultural Research and Development Agency, the Centre for Research  and  Development  of  Food  Crops,  Bogor. Page:205-228.

Bnejdi F, Gazzah ME. 2010. Epistasis and genotype-by-environment interaction of grain yield related traits in durum wheat. Journal Plant Breeding Crop Science 2 (2), 24-29.

Bnejdi F, Hanbary C, Mohamed EG. 2011. Genetic adaptability of inheritance of resistance to biotic and abiotic stress level on crop: Role of epistasis. African Journal of Biotechnology 10(86), 19913-19917.

Delhaize E, Ryan PR, Hebb DM, Yamamoto Y, Sasaki T, Matsumoto H. 2004. Engineering high level aluminum tolerance in barley with the ALMT1 gene. Proceedings of the National Academy of Sciences of the United States of America 101(42),15249-15254.

Caniato FF, Guimaraes CT, Schaffert RE, Alves VMC, Kochian LV, Borem A, Klein PE, Magalhaes JV. 2007. Genetic diversity for aluminum tolerance in sorghum. Theoretical and Applied Genetic 114, 863-876.

[FAO] Food and Agricultural Organization. 2013.

Furukawa J, Yamaji N, Wang H, Mitani N, Murata Y, Sato K, Katsuhara M, Takeda K Ma JF. 2000. An aluminum-activated citrate transporter in barley. Plant Cell Physiology 48, 1081-1091.

Jayaramachandran M, Kumaravadivel N, Eapen S, Kandasamy G. 2010. Gene Action for yield attributing characters in segregating generation (M2) of Sorghum (Sorghum bicolor L.). Electric Journal Plant Breeding 1(4), 802-80.

Karasu A, Oz M, Goksoy AT, Turan ZM. 2009. Genotype by environment interaction, stability and heritability of seed yield and certain agronomical traits in soybean [Glycine max (L.) Merr.]. African Journal of Biotechnology 8(4), 580-590.

Kochian LV, Hoekenga OA, Piñeros MA. 2004. How do crop tolerate acid soils? Mechanism of aluminum tolerance and phosphorous efficiency. Annual Review of Plant Biology 55, 459-493.

Liao H, Wan H, Shaff J, Wang X, Yan X, Kochian LV. 2006. Phosphorus and aluminum interactions in soybean in relation to aluminum tolerance. Exudation of specific organic acids from different regions of the intact root system. Plant Physiology 141, 674-684.

Maron LG, Kirst M, Mao C, Milner MJ, Menossi M, Kochian LV. 2008. Transcriptional profiling of aluminum toxicity and tolerance responses in maize roots. New Phytologist 179, 116–12.

Miftahudin, Scoles GJ, Gustafson JP. 2004. Development of PCR-based codominant markers falnking the Alt3 gene in rye. Genome 47, 231-238.

Murti RH, Kurniawati T, Nasrullah. 2004. The pattern of inheritance tomato fruit characters. Zuriat 15(2), 140-149.

Ohki K. 1987. Aluminum stress on sorghum growth and nutrient relationships. Plant and Soil 98, 195-202.

Pantalone VR, Burton JW, Carter TE. 1996. Soybean fibrous root heritability and genotipic correlation with agronomic and seed quality traits. Crops Science 36, 1120-1125.

Roslim DI, Miftahudin, Suharsono U, Aswidinnoor H, Hartana A. 2010. Character root re-growth as parameters tolerance aluminum on rice. Journal Natur Indonesian 13(1), 82-88.

Roy D. 2000. Plant Breeding: Analysis and Exploitation of Variation. New Delhi: Narosa.

Soil Research Association. 2006. Recommenda-tions fertilizing soybean is on land various types. www.balittanah.litbang.deptan.go,id [10 April 2010].

Silva S, Carnide OP, Lopes PM, Matos M, Pinto HG, Santos C. 2010. Differential aluminium changes on nutrient accumulation and root differentiation in an Al sensitive vs. tolerant wheat. Journal Environmental and Experimental Botany 68(1), 91-98.

Singh RK, Chaudhary BD. 1979. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Pub. Ludhiana, New Delhi.

Singh D, Raje RS. 2011. Genetics of aluminium tolerance in chickpea (Cicer arietinum). Plant Breeding 130(5), 563-568.

Statistic Center Association. 2013. Product, harvest area and palawija productivity on Indonesia. 07/Palawija National.htm [12 April 2013].

Tester M, Bacic A. 2005. Abiotic stress tolerance in grasses. From model plant to crop plants. Plants Physiology 133, 791-793.

Wirnas D, Widodo I, Trikoesoemaningtyas, Sobir, Sopandie D. 2006. Selection agronomic character to arrange index selection on 11 soybean populations F6 generations. Buletin Agronomi 34(1),19-24.

Yang ZM, Sivaguru M, Horst WJ, Matsumoto H. 2000. Aluminium tolerance is achieved by exudation of citric  acid  from  roots  of  soybean (Glycine max). Physiologia Plantarum 110(1), 72-77.

Zhao  Z,  Ma  JF,  Sato  K, Takeda  K.  2003. Differential  Al  resistance  and citrate  secretion  in barley (Hordeum vulgare L.). Planta 217, 794–800.