home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Analyzing winter wheat’s drought stress tolerance via in-vitro and in-vivo screening

Paper Details

Research Paper 17/06/2024
Views (78) Download (13)
current_issue_feature_image
publication_file

Analyzing winter wheat’s drought stress tolerance via in-vitro and in-vivo screening

Beyayna Vahramians Khosravizad, Andreas Melikyan, Hamlet Martirosyan
Int. J. Biosci.24( 6), 184-197, June 2024.
Certificate: IJB 2024 [Generate Certificate]
Key: Drought tolerancePEG-6000Seedling traitsWinter wheatYield

Abstract

Occurrences of osmotic stress have profound impacts on global wheat production. Drought may be a global issue, in any wheat-producing region that can cause severe osmotic stress. In this study, a comparative study of drought tolerance screening techniques in vitro and in vivo was conducted using fifteen winter wheat cultivars. Under in-vitro screening, for simulating drought conditions, -0.45 MPa and -0.9 MPa osmotic potential were used. Polyethylene glycol 6000 was used to induce osmotic stress and seedling traits such as germination (%), shoot length, total root length, total root number, fresh and dry weights of shoots and roots, also proline content were studied. In the case of in vivo experiments, yield-contributing and biochemical traits were measured. The parameters included plant height, plant number, total tiller numbers, spike length, number of grains per spike, 1000-grain weight, grain yield per plant (g), and protein percentage, which were studied under irrigated and water-stress conditions. Drought stress significantly reduced seedling and yield-contributed traits. In the case of biochemical parameters (protein and proline), an increase was observed. The results can provide insight into the root trait development of wheat under -0.45 MPa and guide root architecture optimization and quality improvement in wheat. An analysis of correlations found significant correlations between most of the studied traits. According to the results, the cultivars Navid, Sabalan, Azar2, and Zare were identified as drought-tolerant while the cultivars Mihan, D92, and G31 were observed as drought-sensitive.

VIEWS 30
Cover PDF

Ahmad M, Shabbir G, Minhas NM, Shah MKN. 2013. Identification of drought-tolerant wheat genotypes  based on seedling traits. Sarhad Journal of Agriculture 29, 21-27.

Ahmad NM, Kamil AD. 2017. Early Screening of Some Kurdistan Wheat (Triticum aestivum L.)  genotypes  under Drought Stress. Journal of Agricultural Science 9, 88. DOI: 10.5539/jas. v9n2p88.

Ahmad Z, Waraich EA, Akhtar S. 2018. Physiological responses of wheat to drought stress and its mitigation approaches. Acta Physiologiae Plantarum 40, 80. https://doi.org/10.1007/s11738-018-2651-6

Ahsan J, Nadeem A, Javed A, Amir H, Muhammad AA, Syed AZ, Maqbool A, Hadba Al, Hanan AA, Mohammad S, Al-Harbi EFA. 2022. Grain yield, chlorophyll and protein contents of elite wheat genotypes under drought stress, Journal of King Saud University Science 34(7), 102279. https://doi.org/10.1016/j.jksus.2022.102279.

Almaghrabi OA. 2012. Impact of drought stress on germination and seedling growth parameters of some wheat genotypes. Life Science Journal 9, 590–598.

Andjelkovic V. 2018. Introductory Chapter: Climate Changes and Abiotic Stress in Plants. Plant, Abiotic Stress and Responses to Climate Change. IntechOpen Journal. http://dx.doi.org/10.5772/intechopen.76102

Aneela U, SyedAM, Amjad M. 2017. Hormonal Seed Priming Improves Wheat (Triticum aestivum L.) Field Performance Under Drought and Nonstress Conditions. Pakistan Journal of Botany 49(4), 1239-1253.

Anjum SA, Ashraf U, Zohaib A, Tanveer M, Naeem M, Ali I, Nazir U, Tabassum T. 2017. Growth and developmental responses of crop plants under drought stress: a review. Zemdirbyste-Agriculture 104(3), 267–276. DOI: 10.13080/z-a.2017.104.034.

Ashraf M, Foolad MR. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental Experimental Botany 59, 206-216.

Ashrafi RP, Shaban N. 2014. Study on protein Changes in wheat under drought stress. International Journal of Advanced Biological and Biomedical Research 2(2), 317-320.

Bardees M, Aldesuquy HS. 2017. Impact of osmotic stress on seedling growth observations, membrane characteristics and antioxidant defense system of different wheat genotypes. Egyptian Journal of Basic and Applied Sciences 4, 47-52. DOI: 10.1016/j.ejbas.2016.10.001.

Baloch MS, Nadim MA, Zubair M, Awan IU, Khan EA, Ali S. 2012. Evaluation of wheat under normal and late sowing conditions. Pakistan Journal of Botany 44(5), 1727–1732.

Barnabás B, Jäger K, Fehér A. 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell and Environment 31(1), 11–38.

Bates L, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207.

Bayoumi TY, Eid MH, Metwali EM. 2008. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat  genotypes . African Journal of Biotechnology 7, 2341–2352.

Bhatt GM. 1973. Significance of path coefficient analysis in determining the nature of character association. Euphytica 22, 338-343.

Bhattarai R, Br O, Dhruba BT, Raju K, Ankit O, Manoj S. 2017. Evaluation of Elite Spring Wheat (Triticum aestivum L.)   genotypes  for Yield and Yield Attributing Traits under Irrigated Condition. International Journal of Applied Sciences and Biotechnology 5, 194. DOI:10.3126/ijasbt. v5i2.17615.

Bouslama M, Schapaugh WT. 1984. Stress tolerance in soybean, part 1: evaluation of three screening techniques for heat and drought tolerance. Crop Science journal 24, 933-937.

Bradford MM. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye Binding. Analytical Biochemistry Journal 72, 248-254.

Cedola MC, Iannucci A, Scalfati G, Soprano M, Rascio A. 1994. Leaf morphophysiological parameters as screening techniques for drought stress tolerance in Triticum durum Desf. Journal of Animal Breeding and Genetics 48, 229–235.

Chachar Z, Chachar N, Chachar Q, Mujtaba S, Chachar G, Chachar S. 2016. Identification of drought-tolerant wheat genotypes under water deficit conditions. International Journal of Research Granthaalayah 4, 206–214.

Cochard H, Coll L, Le Roux X, Améglio T. 2002. Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut. Plant Physiology Journal 128, 282–290. DOI: 10.1104/pp.010400

De Dorlodot S, Forster B, Page L, Price A, Tuberosa R, Draye X. 2007. Root system architecture: opportunities and constraints for genetic improvement of crops. Trends in Plant Science journal 12, 474-481.

Dhanda SS, Sethi GS, Behl RK. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science 190, 6-12.

Ding J, Huang Z, Zhu M, Li C, Zhu X, Guo W. 2018. Does cyclic water stress damage wheat yield more than a single stress? PLoS ONE 13, e0195535. DOI: 10.1371/journal.pone.0195535

Djibril S, Mohamed OK, Diaga D, Diegane D, Abaya BF, Maurice S, Alain B. 2005. Growth and Development of Date Palm (Phoenix dactylifera L.) Seedlings Under Drought and Salinity Stresses. African Journal of Biotechnology 4(9), 968–972.

Dolferus R, Thavamanikumar S, Sangma H, Kleven S, Wallace X, Forrest K, Rebetzke G, Hyden M, Borg L, Smith A, Cullis B. 2019. Determining the genetic architecture of reproductive stage drought tolerance in wheat using a correlated trait and correlated marker effect model. G3 Genes Genomes Genetics 9(12), 473–489. DOI: 10.1534/g3.118.200835

Dong B, Zheng X, Liu H, Able JA, Yang H, Zhao H, Liu M. 2017. Effects of Drought Stress on Pollen Sterility, Grain Yield, Abscisic Acid, and Protective Enzymes in Two Winter Wheat  genotypes. Frontiers in Plant Science journal 8, 1008. DOI: 10.3389/fpls.2017.01008

Faisal S, Khan A, Mujtaba SA, Wajid M. 2017. Morpho-physiological assessment of wheat (Triticum aestivum L.)   genotypes  for drought stress tolerance at seedling stage. Pakistan Journal of Botany 49(2), 445-452.

Fernandes RS, de Oliveira VF, Schroeder LD, de Jesus AP, Busanello C, Santos MF, Viana VE, de Oliveira AC, Pegoraro C. 2020. Morphologically accessing wheat   genotypes   in response to drought in early stages of development. Comparative Political Studies Journal 10.

Fraser T, Silk W, Rosr T. 1990. Effect of low water potential on cortical cell length in growing region on maize roots. Plant Physiology journal 93(2), 648–651. https://doi.org/10.1104/pp.93.2.648

Ghosh S, Shahed MA, Robin AHK. 2020. Polyethylene glycol-induced osmotic stress affects germination and seedling establishment of wheat  genotypes . Plant breeding and biotechnology 8(2), 174-185. https://doi.org/10.9787/PBB.2020.8.2.174

Hassini Noureddine B, Abderrahmani M, Belaid AD. 2018. Effects of Drought on the Production of Winter Wheat in Semi-arid Zones. International Journal of Water Resources and Arid Environments 7(2), 153-159.

Hussain S, Hussain S, Qadir T, Khaliq A, Shraf U, Parveen A, Saqib M, Rafiq M. 2019. Drought stress in plants: an overview on implications, tolerance mechanisms, and agronomic mitigation strategies. Plant Science Today Journal 6(4), 389–402. http://dx.doi.org/10.14719/pst.2019.6.4.578

Izabela M, Ilona CM, Edyta S, Maria F, Stanisław G, Maciej TG. 2013. Impact of osmotic Stress on physiological and biochemical characteristics in drought susceptible and drought-resistant wheat   genotypes. Acta Physiologiae Plantarum Journal 35, 451-46. https://link.springer.com/article/10.1007/s11738-012-1088-6

Jajarmi V. 2009. Effect of water stress on germination indices in seven wheat  genotypes. World Academy of Science, Engineering and Technology 49, 105–106. 37.

Jaleel CA, Manivannan PB. 2008. Sankar, A. Kishorekumar, R. Panneerselvam, Salt stress mitigation by calcium chloride in Phyllanthus amarus, Acta Botanica Croatica Journal 67, 53–62.

Ji H, Liu L, Li K, Xie Q, Wang Z, Zhao X, Li X. 2014. PEG-mediated osmotic stress induces premature differentiation of the root apical meristem and outgrowth of lateral roots in wheat. Journal of Experimental Botany 65, 4863–4872.

Kadam S, Shukla Y, Subhash N, Singh C, Suthar K. 2017. Screening of Wheat   genotypes   (Triticum durum L.) in Response to Drought Stress by Some Physiological and Biochemical Indices. International Journal of Pure and Applied Biosciences 5, 969–977. DOI: 10.18782/2320-7051.2795

Kamran M, Shahbaz M, Ashraf M, Akram NA. 2009. Alleviation of drought-induced adverse effects in spring wheat (Triticum aestivum L.) using proline as a pre-sowing seed treatment. Pakistan Journal of Botany 41, 621–632.

Khanna-Chopra R. 2012. Leaf senescence and abiotic stresses share reactive oxygen species-mediated chloroplast degradation. Protoplasma 249, 469–481.

Khatiwada A, Neupane I, Sharma B,  Bhetwal N,  Pandey B. 2020. Effects of Drought Stress on Yield and Yield Attributing Characters of Wheat: A Review. Agriways 8, 118-124. DOI: 10.38112/agw. 2020.v08i02.009.

Kim CS, Kamiya S, Sato T, Utsumi S, Kito M. 1990. Improvement of nutritional value and functional properties of soybean glycinin by protein engineering. Protein Engineering, Design and Selection 3(8), 725-731.

Koç E. 2020. Assessing climate change impacts on wheat production in Turkey and various adaptation strategies, in Climate Change and Food Security with Emphasis on Wheat. eds. M. Ozturk and A. Gul (New York: Elsevier) 43–54.

Kumar N, Nandwal A, Devi S, Sharma K, Yadav A, Waldia R. 2010. Root characteristics, plant water status, and CO2 exchange in relation to drought tolerance in chickpea. Journal of SAT Agricultural Research 8, 5.

Larbi A, Mekliche A. 2004. Relative water content (RWC) and leaf senescence as screening tools for drought tolerance in wheat. Options Mediterranèennes Series A, Seminaires Mediterranèens 60, 193–196. https://doi.org/10.1093/protein/3.8.725

Manivannan P, Jaleel CA, Sankar B, Kishorekumar A, Somasundaram R, Lakshmanan GA, Panneerselvam R. 2007. Growth, biochemical modifications, and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces Journal 59, 141–149. DOI: 10.1016/j.colsurfb.2007.05.002

Maralian F, Ebadi A, Didar TR, Haji-Eghrari B. 2010. Influence of water stress on wheat grain yield and proline accumulation rate. African Journal of Agricultural Research 5, 286–289.

Marci ´nska I, Czyczyło-Mysza I, Skrzypek E, Filek M, Grzesiak S, Grzesiak MT, Janowiak F, Hura T, Dziurka M, Dziurka K. 2013. Impact of osmotic stress on physiological and biochemical characteristics in drought-susceptible and drought-resistant wheat genotypes. Acta Physiologiae Plantarum 35, 451–461.

Michel BE, Kaufmann MR. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology Journal 51(5), 914- 916.

Mujtaba SM, Summiya F, Khan MA, Mumtaz A, Barakat K. 2016. Physiological Studies on Six Wheat (Triticum aestivum L.) Genotypes for Drought Stress Tolerance at Seedling Stage. Agricultural Research & Technology: Open Access Journal 1(2), 1-6.

Naushad AH., Izhar AS, Khan NHI. 2020. Multivariate analysis for various quantitative traits in wheat advanced lines. Saudi Journal of Biological Sciences, 28. DOI:10.1016/j.sjbs.2020.10.011.

Nawaz F, Ahmad R, Waraich EA, Naeem MS, Shabbir RN. 2012. Nutrient uptake, physiological responses, and yield attributes of wheat (Triticum aestivum L.) exposed to early and late drought stress. Journal of Plant Nutrition 35(6), 961-974.

Nonami H. 1998. Plant water relations and control of cell elongation at low water potentials. Journal of Plant Research 111(3), 373-382. https://doi.org/10.1007/bf02507801.

Ojha BR. 2012. On-farm evaluation of yield and yield attributing traits of promising wheat genotypes  at mid-western hills of Nepal. Journal of Plant Breeding 7, 3-14.

Pour-Aboughadareh A, Omidi M, Naghavi MR, Etminan A, Mehrabi AA, Poczai P, Bayat H. 2019. Effect of Water Deficit Stress on Seedling Biomass and Physio-Chemical Characteristics in Different Species of Wheat Possessing the D Genome. Agronomy 9, 522. https://doi.org/10.3390/agronomy9090522

Prakash V, Tiwari S, Shukla RS, Tripathi N, Sapre S. 2015. Evaluation of Drought Stress Tolerance Efficiency of Wheat (Triticum aestivum L.)   genotypes   at Germination and Seedling Stages. International Journal of Bio-resource and Stress Management 6, 41–46.

Rauf M, Munir M, Ul-Hassan M, Ahmed M, Afzal, M. 2007. Performance of wheat  genotypes  under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology 6(8), 971-975.

Robin AHK, Ghosh S, Shahed MA. 2021. PEG-Induced Osmotic Stress Alters Root Morphology and Root Hair Traits in Wheat  genotypes . Plants 10(6), 1042. https://doi.org/10.3390/plants10061042.

Robin AHK, Uddin J, Bayazid N. 2015. Polyethylene Glycol (PEG)-Treated Hydroponic Culture Reduces Length and Diameter of Root Hairs of Wheat Varieties. Agronomy 506-518. DOI: 10.3390/agronomy5040506.

Rogelj J, Den Elzen M, Höhne N, Fransen T, Fekete H, Winkler H. 2016. Paris Agreement climate proposals need a boost to keep warming well below 2 C. Nature 534(7609), 631-639.

Rose G, Osborne T, Greatrex H, Wheeler T. 2016. Impact of progressive global warming on the global-scale yield of maize and soybean. Climatic Change 134(3), 417-428.

Saha RR, Hannan A, Nessa A, Malek MA, Islam MR. 2018. Selection of drought-tolerant wheat  genotypes  by osmotic stress imposed at germination and early seedling stage. SAARC Agriculture Centre 15(2), 177–192. DOI: 10.3329/sja. v15i2.35147.

Sayar R, Bchini H, Mosbahi M, Khemira H. 2010. Response of durum wheat (Triticum durum Desf.) growth to salt and drought stresses. Czech J. Genet. Plant Breed 46, 54-63.

Sharma V, Kumar A, Chaudhary A, Mishra A, Rawat S, B. BY, Shami V, Kaushik P. 2022. Response of Wheat genotypes to Drought Stress Stimulated by PEG. Stresses 2(1), 26-51. https://doi.org/10.3390/stresses2010003

Soltani A, Gholipoor M, Zeinali E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany 55, 195–200.

Thapa DB, Sharma RC, Mudwari A, Ortiz-Ferrara G, Sharma S, Basnet RK, Witcombe JR, Virk DS, and Joshi KD. 2009. Identifying superior wheat genotypes in participatory research on resource poor farms. Field Crops Research 112(2), 124-130. DOI: 10.1016/j.fcr.2009.01.011

Turan F. 2018. Evaluation of seed yield of durum wheat (Triticum durum) under drought stress and determining correlation among some yield components using path coefficient analysis. UNED Research Journal 10. DOI: 10.22458/urj. v10i1.2023.

Vandana Sh, Ashwini K, Astha Ch, Anurag M, Suniti R, Basavaraj YB, Vaishali Sh, Prashant K. 2021. Response of Wheat genotypes  to Drought Stress Stimulated by PEG. Stresses 2, 26–51. https://doi.org/10.3390/stresses2010003

Wang JY, Turner NC, Liu YX, Siddique KH, Xiong YC. 2017. Effects of drought stress on morphological, physiological, and biochemical characteristics of wheat species differing in ploidy level. Functional Plant Biology journal 44(2), 219–234. http://dx.doi.org/10.1071/FP16082

Wang W, Vinocur B, Altman A. 2003. Plant responses to drought, salinity, and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218, 1–14. DOI: 10.1007/s00425-003-1105-5

Zhan HX, Chang ZJ, Wei AL, Zhang XJ, Li X. 2011. Impact of drought to wheat physiological index. Journal of Shanxi Agricultural Sciences 39, 1049–1051.

Submit Manuscript