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Anatomical variation and molecular characterization of Catharanthus roseus through RAPD analysis

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Research Paper 15/10/2022
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Anatomical variation and molecular characterization of Catharanthus roseus through RAPD analysis

S.N. Sima, Tanzin Afsana
Int. J. Biosci.21( 4), 121-132, October 2022.
Certificate: IJB 2022 [Generate Certificate]
Key: Anatomical characterCatharanthus roseus L.Cluster analysisGenetic diversityRAPD

Abstract

Catharanthus roseus is one of the most interesting groups of ornamental plants in the world with high medicinal value and member of the family Apocynaceae. The goal of this study was to investigate the anatomical and molecular characterization of five types (White yellow, White red, Pink yellow, Pink red, and Red yellow) of C. roseus plant. The anatomical study was made by cutting transverse sections of the materials and stained with double stained techniques and observed data with the help of high-powered compound microscope. Molecular analysis was done by using RAPD molecular markers with four primers. Anatomical analysis indicated that the epidermis was recorded as a single layer. Bi-collateral vascular bundle was present and frequently arranged in radial symmetry in the stem. The results of RAPD markers revealed a total of sixty-nine (69) amplified bands, forty-six (46) of them were monomorphic and twenty-three (23) of them were polymorphic from using four primers. The result of dendrogram separated the five types of C. roseus into two major clusters (C1 and C2). Cluster C1 is represented by a single type Red yellow indicating that it is distinct from other four types, and Cluster C2 is represented by the other four types of C. roseus. The dendrogram and linkage distance revealed that the highest similarity was 70% between S1 (White yellow types) and S2 (White red types). However, the lowest similarity was 41.7% between S3 (Pink yellow types) and S5 (Red yellow types). This study could provide a key platform for further crop improvement at molecular level for genetic variability and cross breeding.

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Ahmed MF, Kazim SM, Ghori SS, Mehjabeen SS, Ahmed SR, Ali SM, Ibrahim M. 2010. Antidiabetic Activity of Vinca rosea Extracts in Alloxan-Induced Diabetic Rats. International Journal of Endocrinology 1-6.

Adekomi DA. 2010. Madagascar periwinkle (Catharanthus roseus) enhances kidney and liver functions in Wistar rats, European Journal of Anatomy 14, 111-119.

Arif IA, Bakir MA, Khan HA, Al Farhan AH. 2010. A brief review of molecular techniques to assess plant diversity. International Journal of Molecular Sciences 11, 2079-2096.

Chaudhary AA, Yadav D, Hemant Jamil SS, Asif M. 2012. Real time sequence characterized amplified region (RTSCAR) marker: Development and its application for authentication and quantification of Catharanthus roseus L. Donald Journal for Medicinal Plants 6, 4748-4754.

El-Domyati FM, Ramadan AM, Gadalla NO, Edris S, Shokry AM, Hassan SM, Hassanien SE, Baeshen MN, Hajrah NH, Al-Kordy MA, Abuzinadah OA, AlHajar ASM, Akoh CC, Bahieldin A. 2012. Identification of molecular markers for flower characteristics in Catharanthus roseus producing anticancer compounds. Life Science Journal 9, 5949-5960.

Gathirwa JW, Rukunga GM, Njagi ENM. 2007. In vitro anti-plasmodial and in vivo antimalarial activity of some plants traditionally used for the treatment of malaria by the Meru community in Kenya, Journal of Natural Medicines 61, 261-268.

Jaleel CA, Gopi R, Manivannan P, Panneerselvam. 2008. Soil salinity alters the morphology in Catharanthus roseus and its effects on endogenous mineral constituents. Eurasian Journal of Biosciences 2, 18-25.

Kumar OA, Tata SS, Kumar KP. 2013. Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L.) G. Don.) using Biochemical Markers. Notulae Scientia Biologicae 5, 458-461.

Kalpana J, Warude PC, Bhushan P. 2004. Molecular markers in herbal drug technology. Current Science 87, 159-165.

Kim SW, Ban SH, Jeong SC. 2007. Genetic discrimination between Catharanthus roseus cultivars by metabolic fingerprinting using 1H NMR spectra of aromatic compounds. Biotechnology and Bioprocess Engineering 12, 646- 652.

Lucas DM, Still PC, Pérez LB, Grever MR, Kinghorn AD. 2010. Potential of plant-derived natural products in the treatment of leukemia and lymphoma. Curr. Drug. Targets 11, 812822.

Leveque D, Wihlm J, Jehl F. 1996. Pharmacology of Catharanthus alkaloids. Bull. Cancer 83, 176–186.

Leal AA, Mangolin CA, do Amaral ATJ, Goncalves LS. 2010. Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines. Genetics and Molecular Research 9, 9-18.

Lal S, Mistry KN, Shah SD, Thaker R, Vaidya PB. 2011. Genetic diversity assessment in nine cultivars of Catharanthus roseus from Central Gujarat (India) through RAPD, ISSR and SSR markers. Journal of Biological Research 8, 667-675.

Loh JP, Kiew R, Kee A, Gan LH, Gan YY. 1999. Amplified Fragment Length Polymorphism (AFLP) provides molecular markers for the identification of Caladium biocolor cultivars. Annals of Botany 84, 155-161.

Mishra P, Kumar S. 2000. Emergence of periwinkle Catharanthus roseus as a model system for molecular biology of alkaloids: phytochemistry, pharmacology, plant biology and in vitro and in vivo cultivation. Journal of Medicinal and Aromatic Plant Sciences 22, 306–337.

Nejat N, Maziah M, Valdiani A, Tan YH, Cahill D, Abiri R. 2015. Ornamental exterior versus therapeutic interior of Madagascar periwinkle (Catharanthus roseus): the two faces of a versatile herb. Review Article. The Scientific World Journal ID 982412. http://dx.doi.org/10.1155/2015/982412

Papon N, Bremer J, Vansiri A, Andreu F, Rideau M, Creche J. 2005. Cytokinin and ethylene control indole alkaloid production at the level of the MEP/terpenoid pathway in Catharanthus roseus suspension cells. Planta Medicine 71, 572-4.

Pandiangan D. 2012. The morphological and anatomical changes on tryptophan-treated callus of Catharanthus roseus. Journal of Biologos 2(1), 45-49.

Patil PJ, Ghosh JS. 2010. Antimicrobial activity of Catharanthus roseus-A Detailed Study. British Journal of Pharmacology and Toxicology 1, 40-44.

Plaizier AC. 1981. A revision of Catharanthus roseus (L.) G. Don (Apocynaceae). Meded Landbouwhogeschool 81(9), 1-12.

Pandey-Rai S, Mallavarupa GR, Naqvi AA, Yadav A, Rai SK, Srivastava S. 2006. Volatile compounds of leaves and flowers of periwinkle Catharanthus roseus (L.) G. Don from New Delhi. Flavour and Fragrance Journal 21, 427–430.

Prasad MP. 2014. Detection of genetic diversity of Catharanthus species using RAPD PCR. Indian Journal of Advanced Botany 1, 24.

Renoult J, Nuzillard J, Mare L, Le Crouerour G, Thepeiner P, Harnot Z. 1999. Isolation of indol alkaloids from C. roseus by centrifuged partition chromatography.

Rajaseger G, Tan HTW, Turner IM, Kumar PP. 1997. Analysis of genetic diversity among Ixora cultivars (Rubiaceae) using random amplified polymorphic DNA. Annals of Botany 80, 355-361.

Rajasegar G, Tan HTW, Turner IM, Kumar PP. 1999. Random Amplified Polymorphic DNA variation among and within selected Ixora (Rubiaceae) populations and mutants. Annals of Botany 84, 253-257.

Simpson MG. 2006. Plant systematics. Elsevier, Amsterdam, The Netherlands.

Shaw RK, Acharya L, Mukherjee AK. 2009 Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. Crop breeding and applied biotechnology 9, 52-59.

Tharachand C, Immanuel Selvaraj C, Mythili MN. 2012. Molecular markers in characterization of medicinal plants: An overview. Research in Plant Biology 2, 01-12.

Vega-Avila E, Cano-Velasco JL, Alarcón FJ, Aguilar MDC, Fajardo Ortíz, Almanza Pérez JC, Román-Ramos R. 2012. Hypoglycemic activity of aqueous extracts from Catharanthus roseus, Evidence-Based Complementary and Alternative Medicine 1- 7.

Van der Fits L, Deakin EA, Hoge JH. Memelink J. 2000. The ternary transformation system: constitutive VirG on a compatible plasmid dramatically increases Agrobacterium-mediated plant transformation. Plant Molecular Biology 43, 495–502.

Vardhan S, Yadav AK, Suria RK, Nag BBSP. 2012. Genetic relatedness and In vitro antimicrobial activities of alkaloids isolated from Indian varieties of Catharanthus roseus (L.). Current Botany 3, 43-48.

Wahua C, Okoli BE, Sam SM. 2013. Comparative Morphological, Anatomical, Cytological, and Phytochemical Studies on Capsicum frutescens Linn. and Capsicum annum Linn. (SOLANACEAE); International Journal of Scientific and Engineering Research 4.

Watson L, Dallwitz MJ. 1992. The Families of Flowering Plants. Descriptions illustrations, identification and information Retrieval version alpha 35.

Zietkiewicz ER, Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification. Genomics 20, 176-183.

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