Antagonistic activities of Pseudomonas fluorescens and strain improvement of Rhizobium species

Paper Details

Research Paper 01/12/2011
Views (451) Download (14)
current_issue_feature_image
publication_file

Antagonistic activities of Pseudomonas fluorescens and strain improvement of Rhizobium species

Chinnappan Alagesaboopathi, Thangasamy Selvankumar
Int. J. Biosci.1( 6), 54-63, December 2011.
Certificate: IJB 2011 [Generate Certificate]

Abstract

The Pseudomonas fluoruescens Migula is a well known soil borne and non pathogenic biological control agent. P. fluorescens has been found to show inhibition to the phytopathogens of Rhizoctonia solani Kuhn, Bipolaris oryzae Shoemaker, Cochliobolus lunatus Nelson, Alternaria brassica Berk, Aspergillus niger Van Tiegh, Fusarium oxysporum Schlechtendal and Trichothecium roseum Link. The antagonistic activity of the crude extract was studiedby duel plate and poison plate assays. This results proved that the crude extract have antagonistic potential against fungal pathogens. The crude extract was purified by Thin Layer Chromatography (TLC) and showed the different coloured bands under Ultra Violet (UV). These crude antibiotic substance is also effective against the spores germination of phytopathogens that studied by spore germination assay. The strain improvement was also carried out on the nitrogen fixer Rhizobium species De Lajudie, using plasmid DNA transformation technique. The plasmid DNA from P .fluorescens was transformed to competent cells of Rhizobium species and the transformations was obtained and showed both biological control.

VIEWS 26

Alagesaboopthi C. 1990. Antagonistic potential of Penicillium pinophilum Hedgc to the phytopathogenic fungus Rhizoctonia solani Kühn. M.Phil. Thesis, Bharathiar University, Coimbatore, Tamil Nadu, India.

Alagesaboopathi C. 1994. Biological control of damping-off diseases of cotton seedling. Current science 66, 865-868.

Alagesaboopathi C, Subramanian G. 2007. Biological control of damping-off disease of Gossypium hirsutum L. Seedling. International symposium on biocontrol and biotechnology, Khonkaen University, Nong Khai Campus, Nong Khai, Thailand, p.67.

Anand T, Chandarasekaran A, Kuttalam S, Senthilraja G, Samiyappan R. 2010. Integrated control of fruit rot and powdery mildew of chilli using the biocontrol agent Pseudomonas fluorescens and a chemical fungicide. Bio Control 52, 1-7.

Anitha A, Arun Das M. 2011. Activation of rice plant growth against Rhizoctonia solani using Pseudomonas fluorescens, Trichoderma and salicylic acid. Research in Biotechnology 2, 7-12.

Banasco P, Funente L, Dela, Gaultieri G, Noya F, Arias A. 1998. Fluorescent Pseudomonas species. As biocontrol agents against forage legume root pathogenic fungi. Soil Biol Biochem 10, 1317-1323.

Casse F, Boucher C, Juliot JS, Michel M, Denarie J. 1979. Identification and characterization of large plasmids in Rhizobium species using agarose gel electrophoresis. J Gen Microbiol 113, 229-243.

David M. Weller. 2007. Pseudomonas biocontrol agents of soil borne pathogens: looking back over 30 years. Phytopathology 97, 250-256.

Dev N, Dawande AY. 2010. Biocontrol of soil borne plant pathogen Rhizoctonia solani using Trichoderma spp. and Pseudomonas fluorescens. Asiatic J. Biotech. Res. 1, 39-44.

Downing KJ, Thomason JA. 2000. Introduction of the Serratia marcescens Chi A gene into a endophytic P.fluorescens for the biocontrol of phytopathogenic fungi. Can J Microbiol 46, 363-369.

Elad Y, Hadar Y, Hader E, Chet I, Henis. 1981. Biological control of Rhizoctonia solani by Trichoderma harzianum in carnation. Plant Dis. 65, 675-677.

El-Mohamedy RSR, El-Samad A, Hoda AM, Habib TSH, El-Bab F. 2011. Effect of using biocontrol agents on growth, yield, head quality and root rot control in Broccoli plants. International Journal of Academic Research 3, 71-80.

Ganeshan GA, Manojkumar A. 2005. Pseudomonas fluorescens a potential bacterial antagonist to control plant diseases. Journal of Plant Infection 1, 123-131.

Goud MJ, Muralikrishnan V. 2009. Biological control of three phytopathogenic fungi by Pseudomonas fluorescens isolated from rhizosphere. The Internet Journal of Microbiology 7(2).

Harman GE, Chet I and Baker R. 1981. Factors affecting Trichoderma hamatum applied to seeds as biocontrol agent. Phytopathology 71, 569-572.

Hartley C. 1921. Damping – off in forest nurseries. US Dept Agric Bull 934, 1-99.

Henry AW. 1931. The natural microflora of the soil in relation to the foot-rot problem of wheat. Can Jour Res C 4, 69-77.

King EO, Ward MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanine and fluorescein. J Lab Clin Med 44, 301-307.

Kloepper JW, Leong J, Teintzae M, Schroth M.N. 1981. Pseudomonas siderophores: a mechanism explaning disease- suppressive soils. Curr Microbiol 4, 317-320.

Kraus J, Loper J. 1995. Characterization of genomic region required for production of antibiotic pyoluteorin by the biological control agent Pseudomonas fluorescens Pf-5. Appl Environ Microbial 61, 849-854.

Krishnamurthy K, Gnanamanickam SS. 1998. Biological control of rice blast by Pseudomonas fluorescens strain Pf7-14: evaluation of a marker gene and formulations. Biological Control 13, 158-165.

Labeda DP, Liu K, Casida LE. 1976. Colonization of soil by Arthrobacter and Pseudomonas under varying conditions of nutrient and water availability as studied by plate counts and transmission electron microscopy. APP Environ. Microbiol. 31, 551-561.

Manniatis DA, Fritsch EF, Sambrook J. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor. New York.

Menzies JD. 1959. Cooccwence and transfer of biological factor in soil that suppresses potato scab. Phytopathology 49, 648-652.

Negi YK, Garg SK, Kumar J. 2005. Cold-tolerant fluorescent Pseudomonas isolates from Garhwal Himalayas as potential plant growth promoting and biocontrol agents in pea. Current Science 89, 2151-2156.

Nelson EB, Kuter JA, Hortink HAJ. 1983. Effects of fungal antagonists and compost age on suppression of Rhizoctonia damping-off in container media amended with composed hardwood bark. Phytopathology 73, 1457-1462.

Pierson EA, Weller DM. 1994. Use of mixture of fluorescent Pseudomonas to suppress take-all and improve growth of wheat. Phytopathology 84, 940-947.

Rodriguez H, Fraga R. 1999. Phosphate solubiling bacteria  and  their  role  in  plant  growth  promotion. Biotechnol. Adv 17: 319-339.

Selvankumar T, Alagesaboopathi C, Premanand B. 2007. Seed bacterization and biocontrol activity of Pseudomonas fluorescens. Ecology, Environment and Conservation 13, 235-239.

Seong KY, Shin PG. 1996. Effect of siderophore on biological control of plant pathogens and promotion of plant growth by Pseudomonas fluorescens ps 88. Agri Chem Biotechnol 39, 20-24.

Singh A, Mehta S, Singh HB, Nautiyal CS. 2003. Biocontrol of collar rot disease of betelvine Piper betel L. caused by Sclerotium rolfsii by using rhizosphere – competent Pseudomonas fluorescens NBRI-N6 and Pseudomonas fluorescens NBRI-N. Curr. Mocrobiol, 47, 153-158.

Thomashow LS, Weller DM, Bonsall RF, Pierson LS. 1990. Production of the antibiotic phenazine -1-carboxilic acid by fluorescent Pseudomonas species in the rhizosphere of wheat. APPI Environ Microbiol 56, 908-912.

Tombolini R, Van der Gaag DJ, Gerhardson B, Jansson JK. 1999. Colonization pattern of the biocontrol strain Pseudomonas chlororaphis MA 342 on barley seeds visulalized by using green fluorescent protein. APPI Environ Microbial 65, 3674-3680.

Toohey JI, Nelson CD, Krotkov G. 1965. Isolation and identification of two phenazines from a strain of Pseudomonas aureofaciens Can J Botany 43, 1055-1062.

Toyata  K,  Miyashhita  K,  Krimura  M.  1994. Introduction of Chitinase gene into Pseudomonas stutzer A 18 isolated from the surface of chlamydospores of Fusarium oxysporum F. species raphani. Soil Biol Biochem 26, 413-416.

Ursula SK, Arnaud S, Monika M, Caroline B, Brion D, Cecile GB, Cornelia R, Regina N, Genevie VDF, Dieter H, Christoph KL. 2000. Autoinduction of 2,4 Diacetyl phloroglucinol biosynthesis in biocontrol agent Pseudomonas fluorescens CHAO and repression by the bacterial metabolites salicylate and pyoluteorin. Journal of Bacteriology 182, 1215-1225.

Utkhede RS, Gaunce AP. 1986. Inhibition of Phytophathora cacforum by a bacterial antagonist. Can J Botany 61, 3343-3348

Weller DM. 1980. Biological control of soilborne pathogens in the rhizophere with bacteria. Annu Rev Phytopathol 26 : 379-407.

Weller DM, Landa BB, Mavrodi OV, Schroeder KL, De La Fuente L, Bankhead SB, Molar RA, Bonsall RF, Mavrodi DM, Thomashow LS. 2007. Role of 2,4-diacetyl phloroglucinol producing fluorescent Pseudomonas SPP. in plant Defense. Plant Biology 9, 4-20.

Wells HD, Bell DK, Jaworski CA. 1972. Efficacy of Trichoderna harzianum as a biocontrol for Sclerotium rolfsii. Phytopathology 62, 442-447.