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Research Paper | November 1, 2015

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Anti-angiogenic and non-cytotoxic potentials of aqueous and acetone extracts of the stem of Philippine Forest Liana, Bauhinia integrifolia Roxb

Leila A. Allado-Ombat, Franco G. Teves

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Int. J. Biosci.7(5), 184-191, November 2015

DOI: http://dx.doi.org/10.12692/ijb/7.5.184-191

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Abstract

The decoction of Bauhinia integrifolia stem is recommended by Manobo tribe herbalists to treat relapse that are experienced by most mothers after child birth while some used it as abortifacient and birth control. To test its anti-angiogenic and cytotoxic potentials, the bioactive compounds in the stem of B. integrifolia were extracted using 70% acetone and water through decoction. The anti-angiogenic activity of the extracts was assessed using duck chorion-allatoic membrane. The result of the assay showed that both acetone and aqueous extracts exhibited dose dependent anti-angiogenic activity. As the extracts’ concentration increased from 10 mg ml-1 to 1000 mg ml-1, the formation of new blood vessels decreased. However, acetonic extracts showed significantly more lower blood vessel formation compared to the decoction. The evaluation of the cytotoxic activity of the stem extracts employing brine shrimp lethality assay, showed that both extracts are non- cytotoxic since the acetonic extract has LC50 of 9,444 µg ml-1 and the nauplii survived in all concentrations of aqueous extract. With the above results, it is concluded that the extract of B. Integrifolia is non-cytotoxic anti-angiogenic agent. The isolation and further test of the active component possessed by this plant are recommended.

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Anti-angiogenic and non-cytotoxic potentials of aqueous and acetone extracts of the stem of Philippine Forest Liana, Bauhinia integrifolia Roxb

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