Anti-hyperglycemic and anti-hyperlipidemic effects of Panax ginseng root extract in alloxan induced diabetic rats

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Research Paper 01/11/2018
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Anti-hyperglycemic and anti-hyperlipidemic effects of Panax ginseng root extract in alloxan induced diabetic rats

Mahrukh Naseem, Nayab Khan, Sajid Khan Tahir, Ghulam Dastagir, Tahseen Ara, Saeed Ahmed Essote
Int. J. Biosci.13( 5), 27-33, November 2018.
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Abstract

Diabetes is a third leading life threatening metabolic disorder. Hyperglycemia, dyslipidemia, myocardial infarction and oxidative stress are the major reasons of mortality and morbidity. In the present study we try to evaluate the anti-diabetic and anti-hyperlipidemic properties of Panax ginseng root extract (PG). Wistar-male rats were fed on high fat died for 14-days followed by single intraperitoneal dose of alloxan (130mg/kg BW). Hypoglycemia was prevented by giving glucose solution to drink; finally fasting blood glucose was measured after three-days and rats showed blood glucose level higher than 250mg/dl were included in the study. The rats were divided into four groups: Group-1: included non-diabetic rats, Group-II included diabetic rats without any treatment of PG, Group-III: included diabetic rats receiving 150mg/kg BW of PG, Group-IV: included diabetic rats treated with 250mg/kg BW.  Rats were treated for 14 weeks. ANOVA followed by PLSD Fisher’s test was applied. Body weight and blood glucose was measured on 1st and 14th week. We found a significant reduction in blood glucose and fasting-serum-glucose for group III (306.13±14.14, 273.63±7.90) and for group IV (402.97±12.76; 220.87±9.34) respectively. We found a significant reduction for TC (1.23±0.02), VLDL (0.26±0.01), LDL (0.46±0.01) and significant increased for HDL (0.60±0.01) in Group-IV. Significant increase was found for TG in group III (1.54±0.01) and group IV (1.40±0.01). A significant increased for CAT (19.74±0.58) and significant decrease for MDA (6.56±0.23) was found in group-IV. Furthermore a significant decrease for AST, ALT and creatinine level after treatment. In conclusion PG showed strong anti-hyperglycemic, anti-hypercholesterolemic, anti-hypertriglyceridemic and anti-oxidative properties in dose dependent manner.

VIEWS 17

Amin KA, Awad EM, Nagy MA. 2011. Effects of Panax quinquefolium on Streptozotocin-induced diabetic rats: role of C-peptide, nitric oxide and oxidative stress. International Journal of Clinical and Experimental Medicine 4(2), 136-147.

Badish JG, Pacioretty LM, Bland JS, Minich DM, Hu  J, Tripp ML. 2010. Antidiabetic screening of commercial botanical products in 3T3-L1 adipocytes and db/db mice. Journal of Medicinal Food 13(3), 535-547.

Benzi G, Morretti A. 1995. Are reactive oxygen species involved in Alzheimer’s disease. Neurobiology of Aging 16(4), 661–674.

Bhor VM, Raghuram N, Sivakami S. 2004. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. International Journal of Biochemistry and Cell Biology 36(1), 89-97.

Cheng D, Liang B, Yunhui L. 2013. Antihyperglycemic effect of Ginkgo biloba extract in Streptozotocin-induced diabetes in rats. Biomed Research International 2013(1), 1-7.

Cho WCS, Chung W, Lee SKW, Leung AWN, Chen CHK, Yue KKM. 2006. Gisenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin induced diabetic rats European Journal of Pharmocology 550(1), 173-279.

Deisseroth A, Dounce AL. 1970. Catalase physical and chemical properties, mechanism of catalysis, and physiological role. Physiological Reviews 50(3), 319-375.

Ebueli OAT, Ajuluchukwu AE, Afolabi OT, Akinwande AI. 2010. Oxidative stress in alloxan-induced diabetes in female and male rats. Advances in Medical and Dental Sciences 3(3), 71-75.

El-Mesallamy HO, Metwally NS, Soliman MS, Ahmed KA, Moaty MMA. 2011. The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats. Cancer Cell International 11(1), 38- 49.

Ene AC, Nwankawo EA Samdi LM. 2007. Alloxan-induced diabetes in rats and the effect of the black caraway (Carum carvi L.) oil on their body weight. Research Journal of Medical Sciences 2, 48-52.

Halliwell B, Gutterridge JMC. 1999. Role of free radicals in neurodegenerative disease: therapeutic implications for antioxidant treatment. Drugs Aging 18(9), 685-716.

Ibrahim MA, Ibrahim MH, Ayoub HA. 2008. Diabetes mellitus as an oxidative stress. Romanian Journal of Biophysics 18(3), 195-208.

Jung C, Seog HM, Choi IW, Choi HD, Cho HY. 2005. Effects of wild ginseng (Panax ginseng C.A. Meyer) leaves on lipid peroxidation levels and antioxidant enzyme activities in streptozotocin diabetic rats. Journal of Ethnopharmacology 98(3), 245-250.

Jung HL, Kang HY. 2013. Effects of Korean red ginseng supplementation on muscle glucose uptake in high-fat fed rats. Chinese Journal of Natural Medicine 11(5), 494-499.

Kim YJ, Jeon JN, Jang MG, Oh JY, Kwon WS, Jung SK, Yang DC. 2014. Ginsinoside profiles and related gene expression during foliation in Panax ginseng Meyer. Journal of Ginseng Research 38(1), 66-72.

Kodydkova J, Vavrova L, Kocik M, Zak A. 2014. Human catalase, its polymorphism, regulation and changes of its activity in different diseases. Folia Biologica 60(4), 153-167.

Krishnamurthy G, Lakshman K, Pruthvi N  Chandrika PU. 2011. Antihyperglycemic and hypolipidemic activity of methanolic extract of Amaranthus viridis leaves in experimental diabetes. Indian Journal of Pharmacology 43(4), 450-454.

Kumar R. 2012. Correlation of Selenium and other antioxidants in diabetic patients with and without complications. Free Radicals and Antioxidants 2(1), 6-8.

Kunutsor SK, Apekey TA, walley J. 2013. Liver aminotransferase and risk of the incident type 2 diabetes: A systematic review and Meta-analysis. American Journal of Epidemiology 178(2), 159-171.

Lee HJ, Lee YH, Park, SK, Kang ES, Kim HJ, Lee YC, Choi CS, Park SE, Ahn CW, Cha BS, Lee KW, Kim KS, Lim SK, Lee HC. 2009. Korean red ginseng (Panax ginseng) improves insulin sensitivity and attenuates the development of diabetes in Otsuka long-Evans Tokushima fatty rats.  Journal of Metabolism 58(8), 1170-1177.

Lee SH, Lee HJ, Lee YH, Lee BW, Cha BS, Kang ES, Ahn CW, Park JS, Kim HJ, Lee EY, Lee HC. 2012. Korean red ginseng (Panax ginseng) improves insulin sensitivity in high fat fed Sprague-Dawley rats. Phytotherapy Research 26(1), 142-147.

Lim S, Yoon JW, Choi SH, Cho BJ, Kim JT, Chang HS, Park HS, Park KS, Lee HK, Kim YB, Jang HC. 2009. Effect of ginsam, a vinegar extract from Panax ginseng, on body weight and glucose homeostasis in an obese insulin-resistant rat model. Metabolism- Clinical and Exprimental 58(1), 8-15.

Liu Z, Li W, Li X, Zhang M, Chen l, Zheng YN, Sun GZ,  Ruan CC. 2013. Antidiabetic effects of malonyl ginsenosides from Panax ginseng on type 2 diabetic rats induced by high-fat diet and streptozotocin. Journal of Ethnopharmacology 145(2), 233-240.

Li-Xia Y, Tong-hua L, Zong TH, Juan EL, Li LW. 2011. Research progress on the mechanism of single Chinese medicinal herbs in treating diabetes mellitus. Chinese Journal of Integrative Medicine 17(3), 235-240.

Murphy LL, Lee TJ. 2002. Ginseng, sex behaviour and nitric oxide. Annals of New York Academy of Sciences 962, 372-377.

Naseem M, Nazih H, Ouguerram K, Rabbani I, Zaneb H, Rehman HU, Masood I, Michel J, Yousaf MS Tahir SK. 2016. The effects of Panax ginseng root extract on carbohydrate and lipid disturbances associated to alloxan-induced diabetic rats. Journal of Animal and Plant Sciences 26(5), 1218-1225.

Nayak BS, Roberts L. 2006. Relationship between inflammatory markers, metabolic and anthropometric variables in the Caribbean type 2 diabetic patients with and without microvascular complications. Journal of Inflammation 3, 17.

Ohnishi Y, Takagi S, Miura T, Usami M, Kako M, Ishihara E, Yano H, Tanigawa K, Seino Y. 1996. Effect of ginseng radix on GLUT2 protein content in mouse liver in normal and epinephrine-induced hyperglycemic mice. Biological and Pharmaceutical Bulletin 19(9), 1238-1240.

Radad K, Gille G, Liu L, Rausch WD. 2006. Use of Ginseng in medicine with emphasis on neurodegenerative disorders. Journal of Pharmacological  Sciences 100(3), 175-186.

Ramadan G, El-Beih NM, El-Ghaffar AEA. 2009. Modulatory effects of the black V. green tea aqueous extract on hyperglycemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models. British Journal of Nutrition 102(11), 1611-1619.

Ren M, Yang S, Li J, Hu Y, Ren Z, Ren S. 2013. Ginkgo biloba L. extract enhances the effectiveness of syngeneic bone marrow mesenchymal stem cells in lowering blood glucose levels and reversing oxidative stress. Endocrinology 43(2), 360-369.

Ripsin CM, Kang H, Urban RJ. 2009. Management of blood glucose in type 2 diabetes mellitus. American Family Physician 79(1), 29-36.

Salih NA. 2012. Effect of ginseng (Panax ginseng) on experimentally induced diabetes mellitus in male rabbits. Al-Anbar Journal of Veterinary Sciences 5(1), 188-194.

Schwartz SL. 2006. Diabetes and dyslipidaemia. Diabetes Obesity Metabolism 8(4), 355-364.

Son SM. 2012. Reactive oxygen and nitrogen species in pathogenesis of vascular complications for diabetes. Journal of Diabetes and Metabolism 36(3), 190-198.

West IC. 2000. Radicals and oxidative stress in diabetes. Diabetic Medicine 17(3), 171-180.

Wild S, Roglic G, Green A, Sicree R King H. 2004. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 27(5), 1047-1053.

Zheng XK, Zhang L, Wang WW, Wu YY, Zhang Q, Feng WS. 2011. Anti-diabetic activity and potential mechanism of total falvonoids of Selaginella trmariscina (Beauv). Spring in rats induced by high fat diet and low dose STZ. Journal of Ethnopharmacology 137(1), 662-668.

Zohary D, Hopf M. 2000. Domestication of Plants in the Old World. Oxford: Oxford University Press, New York, p 122.