home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Anti-inflammatory properties of Uvaria chamae used in traditional Beninese medicine (West Africa)

Paper Details

Research Paper 07/01/2024
Views (400) Download (68)
current_issue_feature_image
publication_file

Anti-inflammatory properties of Uvaria chamae used in traditional Beninese medicine (West Africa)

Lauris Fah, Jean Robert Klotoé, Eric Agbodjento, Victorien tamègnon Dougnon, Moïse Glèlè, Kévin Sintondji, Phénix Assogba, Esther Déguénon, Akadiri Yessoufou
J. Bio. Env. Sci.24( 1), 40-50, January 2024.
Certificate: JBES 2024 [Generate Certificate]
Key: Anti-inflammatory activityTNFαUvaria chamae

Abstract

Inflammatory diseases are still a major public health concern. Their management is problematic due to the harmful side-effects of anti-inflammatory drugs traditionally used in conventional medicine. Medicinal plants are a credible source for research into new anti-inflammatory drug. This study aimed to explore the anti-inflammatory properties of the roots of Uvaria chamae. Anti-inflammatory activity was explored using the red blood cell membrane stability test and the formalin-induced œdema model in rats. TNFα assay was used to assess immunomodulatory activity. This methodology was complemented by research into antioxidant power using the DPPH test. Results indicated that the extract significantly inhibited membrane destabilization of red blood cells (61.43%), and the formation of formalin-induced paw œdema. This effect is consistent with the reduced production of TNFα (22.06%). The extract tested proved to be a good antioxidant by inhibiting the DPPH radical (IC50= 0.669 mg/mL); its larvae toxicity was moderate (LC50 = 0.033 mg/mL). These results suggest that the ethanolic extract of U. chamae roots has anti-inflammatory, immunomodulating and antioxidant properties. This justifies the use of the plant in traditional Beninese medicine for the treatment of inflammatory diseases.

VIEWS 169
Cover PDF

Agbodjento E, Klotoé JR, Sacramento TI, Dougnon TV, Déguenon E, Agbankpé J, Fabiyi K, Assogba P, Hounkanrin MP, Akotegnon R, Dougnon TJ, Atègbo JM. 2020. Larval Cytotoxic and Subacute Toxicity of Gardenia ternifolia, Rourea coccinea, and Cassytha filiformis Used in Traditional Medicine of Benin (West Africa). Journal of Toxicology 2020, 11. https://doi.org/10.1155/2020/8843575

Al-Shidhani A, Al-Rawahi N, Al-Rawahi A. 2015. Non-steroidal Anti-inflammatory Drugs (NSAIDs) Use in Primary Health Care Centers in A’Seeb, Muscat: A Clinical Audit. Oman Medical Journal 30(5), 366–371. https://doi.org/10.5001/omj.2015.73

Azab A, Nassar A, Azab AN. 2016. Anti-Inflammatory Activity of Natural Products. Molecules 21(10). https://doi.org/10.3390/molecules21101321

Aziz N, Kim MY, Cho JY. 2018. Anti-inflammatory effects of luteolin: A review of in vitro, in vivo, and in silico studies. Journal of Ethnopharmacology 225, 342–358. https://doi.org/10.1016/j.jep.2018.05.019

Chen D, Bi A, Dong X, Jiang Y, Rui B, Liu J, Yin Z, Luo L. 2014. Luteolin exhibits anti-inflammatory effects by blocking the activity of heat shock protein 90 in macrophages. Biochemical and Biophysical Research Communications 443 (1), 326–332. https://doi.org/10.1016/j.bbrc.2013.11.122

Dougnon V, Hounsa E, Eric A, Lunga P, Legba BB, Sintondji KM, Afaton A, Klotoé J, Baba-Moussa L, Bankolé H. 2021. Percentage Destabilization Effect of Some West African Medicinal Plants on the Outer Membrane of Various Bacteria Involved in Infectious Diarrhea. BioMed Research International, 12. https://doi.org/10.1155/2021/4134713

Emordi JE, Agbaje EO, Oreagba IA, Iribhogbe OI. 2018. Antidiabetic Effects of the Ethanolic Root Extract of Uvaria chamae P. Beauv (Annonaceae) in Alloxan-Induced Diabetic Rats: A Potential Alternative Treatment for Diabetes Mellitus. Advances in Pharmacological and Pharmaceutical Sciences, 1314941. https://doi.org/10.1155/2018/1314941

Falade OS, Otemuyiwa IO, Oladipo A, Oyedapo OO, Akinpelu BA, Adewusi SRA. 2005. The chemical composition and membrane stability activity of some herbs used in local therapy for anemia. Journal of Ethnopharmacoly 102, 15–22. https://doi.org/10.1016/j.jep.2005.04.034

Fanou BA, Klotoe JR, Dougnon V, Assogba P, Agbodjento E, Koudokpon CH, Fah L, Sintondji K, Kpoze R, Loko F. 2022. Efficacy of Extracts of Cyanthillium Cinereum, Khaya senegalensis and Lippia multiflora on Candida Strains Isolated From Urine Samples in Benin (West Africa). Frontiers in Tropical Diseases.

Foughalia A. 2017. Évaluation de l’activité anti-inflammatoire de l’extrait brut de la graisse de la bosse de Camelus dromedarius sur un modèle murin  d’arthrite expérimentale (Mémoire de Master). Université des Frères Mentouri Constantine, République Algérienne Démocratique Et Populaire. p.154.

Heymonet C. 2013. Les plantes à visée anti-inflammatoire utilisées en phytothérapie (Thèse de pharmacie). Universite De Lorraine. p.202.

Imran M, Rauf A, Abu-Izneid T, Nadeem M, Shariati MA, Khan IA, Imran A, Orhan IE, Rizwan M, Atif M, Gondal TA, Mubarak MS. 2019. Luteolin, a flavonoid, as an anticancer agent: A review. Biomedicine & Pharmacotherapy 112, 108612. https://doi.org/10.1016/j.biopha.2019.108612

Klotoé J, Fanou B, Eric A, Houehou A, Fah L, Dougnon V, Assogba P, Loko F. 2021. Antifungal activity of Ocimum gratissimum L., Lantana camara L. & Pteleopsis suberosa Engl. & Dies used in the treatment of vulvovaginal candidiasis in Benin. Future Journal of Pharmaceutical Sciences 7. https://doi.org/10.1186/s43094-021-00383-4

Kouchadé AS, Adomou AC, Tossou GM, Yédomonhan H, Dassou GH, Akoègninou A. 2016. Étude ethnobotanique des plantes médicinales utilisées dans le traitement des maladies infantiles et vendues sur les marchés au sud du Bénin. Journal of Animal & Plant Sciences 28, 4418–4438.

Koudokpon H, Armstrong N, Dougnon TV, Fah L, Hounsa E, Bankolé HS, Loko F, Chabrière E, Rolain JM. 2018. Antibacterial Activity of Chalcone and Dihydrochalcone Compounds from Uvaria chamae Roots against Multidrug-Resistant Bacteria. Biomed Research International, 1453173. https://doi.org/10.1155/2018/1453173

Kumar S, Pandey AK. 2013. Chemistry and Biological Activities of Flavonoids: An Overview. The Scientific World Journal. https://doi.org/10.1155/2013/162750

Okwuosa O, Chukwura EI, Chukwuma G, Okwuosa C, Enweani I, Agbakoba N,  Chukwuma C, Manafa P, Umedum C. 2012. Phytochemical and antifungal activities of Uvaria chamae leaves and roots, Spondias mombin leaves and bark and Combretum racemosum leaves. African Journal of Medicine and Medical Sciences 41 Suppl, 99–103.

Mohanty SK, Swamy MK, Middha SK, Prakash L, Subbanarashiman B, Maniyam A. 2015. Analgesic, Anti- inflammatory, Anti- lipoxygenase Activity and Characterization of Three Bioactive Compounds in the Most Active Fraction of Leptadenia reticulata (Retz.)Wight & Arn. A Valuable Medicinal Plant. Iranian Journal of Pharmaceutical Research 14, 933–942.

Monon K, Touré A, Karamoko O, Coulibaly A. 2015. Phytochemical composition, antioxidant and antibacterial activities of root of Uvaria chamae p. Beauv. (Annonaceae) used in treatment of dysentery in north of Côte d’ivoire. International Journal of Pharmacognosy and Phytochemical Research 7, 1047–1053.

Mousseux M. 1995. Test de toxicité sur les larves d’Artemia salina et d’entretien d’un élevage debalanes (Rapport de stage de deuxième année. DEUST Aquaculture). Centre Universitaire de Nouvelle-Calédonie, France, p75.

Nabavi SF, Braidy N, Gortzi O, Sobarzo-Sanchez E, Daglia M, Skalicka-Woźniak K, Nabavi SM. 2015. Luteolin as an anti-inflammatory and neuroprotective agent: A brief review. Brain Research Bulletin 119, 1–11. https://doi.org/10.1016/j.brainresbull.2015.09.002

Nwaehujor CO, Ode JO, Akande MG. 2013. In vitro antioxidant potentials of some herbal plants from southern Nigeria. Journal of Medical Sciences 13, 56–61.

Okokon JE, Ita BN, Udokpoh AE. 2006. The in vivo antimalarial activities of Uvaria chamae and Hippocratea africana. Annals of Tropical Medicine & Parasitology 100, 585–590. https://doi.org/10.1179/136485906X118512

Oluremi BB, Michael O, Omofuma O. 2010. Comparative assessment of antibacterial activity of Uvaria chamae parts. African Journal of Microbiology Research 4(13), 1391–1394.

Oteiza PI, Erlejman AG, Verstraeten SV, Keen CL, Fraga CG. 2005. Flavonoid-membrane interactions: a protective role of flavonoids at the membrane surface? Journal of Immunology Research 12, 19–25.

Pérez-Guerrero C, Herrera MD, Ortiz R, Alvarez de Sotomayor M, Fernández MA. 2001. A pharmacological study of Cecropia obtusifolia Bertol aqueous extract. Journal of Ethnopharmacology 76, 279–284.

Peters-Golden M, Henderson WR. 2007. Leukotrienes. New England Journal of Medicine 357, 1841–1854. https://doi.org/10.1056/NEJMra071371

Rahmani S, Belboukhari N, Sekkoum K, Cheriti A. 2016. Evaluation De L’activité Anti-Inflammatoire D’extraits Aqueux De Feuilles Limoniastrum feei  (Plumbaginacea). Algerian Journal of Arid Environment 6, 80–86.

Sahlmann CO, Ströbel P. 2016. Pathophysiology of inflammation. Nuklearmedizin 55, 1–6.

Santos DYAC dos, Salatino MLF. 2000. Foliar flavonoids of Annonaceae from Brazil: taxonomic significance. Phytochemistry 55, 567–573.

Singh S, Bani S, Singh GB, Gupta BD, Banerjee SK, Singh B. 1997. Anti-inflammatory activity of lupeol. Fitoterapia 68, 9–16.

Singla AK, Pathak K. 1990. Topical antiinflammatory effects of Euphorbia prostrata on carrageenan-induced footpad œdema in mice. Journal of  Ethnopharmacology 29, 291–294.

Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. 1993. A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta medica 59, 250–252.

Thomas PS, Essien EE. 2018. Antiglycation, antioxidant, and cytotoxic activities of Uvaria chamae root and essential oil composition. Natural Product Research 34(6), 1–4. https://doi.org/10.1080/14786419.2018.1504048

Wiya C, Saenphet K, Saenphet S, Buncharoen W. 2018. In vitro analysis of antispasmodic activity of ethanolic stem extracts of Uvaria rufa Blume and Anomianthus dulcis (Dunal) J. Sinclair on excised rat’s ileum. Comparative Clinical Pathology 27, 295–299.

Submit Manuscript