Int. J. Micro. Myco.13( 1), 9-17, July 2021
Antibiotic resistance was firstly observed soon after Alexander Fleming discovered penicillin in 1928. Resistance to commonly used antibiotics is one of the most threatening issues to public health across the globe. There has been an increasing demand to search for novel antimicrobial agents to combat the growing resistance. Medicinal plants are a rich source of many drugs especially antimicrobial drugs and these plants have been used from years to treat different types of diseases. The main goal of our study was evaluation of antimicrobial activity of the selected plant extracts against clinical isolates and then to compare resistance level of bacteria to commonly used antibiotics and medicinal plants. Methanolic extract was taken and dried in water bath; dried extracts were dissolved in Dimethyl Sulfoxide (DMSO) in different concentration (25mg/mL, 50mg/mL, and 100mg/mL). The zones of inhibition were measured by using agar well diffusion method. Methanolic Extracts of Paganum harmala, Fogonia cretica, Ajuga bracteosa and Olea feroogenia showed varying level of activities against E. coli. The results of the study shows that the antibacterial effect of selected plant extracts increased with increase in concentration. The extracts also showed activity against those isolates, which shows resistance to commonly used antibiotics. Therefore, it is suggested that antibacterial agents present in these plants should be isolated through advanced biochemical techniques such as HPLC for the evaluation of potency. These compounds may be evaluated as potential antimicrobial therapeutic agents against a wide range of human pathogens.
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