Antibacterial and antibiofilm properties of methanolic and ethanolic extracts of medicinal plant Rhazya stricta against methicillin-resistant Staphylococcus aureus

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Research Paper 03/02/2024
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Antibacterial and antibiofilm properties of methanolic and ethanolic extracts of medicinal plant Rhazya stricta against methicillin-resistant Staphylococcus aureus

Jamal A. Al-Orabi, Mohsen Mohamed Elsharkawy
Int. J. Biomol. & Biomed.18( 1), 9-19, February 2024.
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Abstract

MRSA (Methicillin-resistant bacteria Staphylococcus aureus) is the main causative agent of chronic infections associated with biofilms in humans, which are responsible for serious healthcare problems in the world. Biofilm-associated infections are challenging to manage with traditional antibiotic treatments due to the protective nature of a surrounding extracellular matrix. In this research, 16S rDNA  of thirty S. aureus strains (isolated from King Faisal Hospital) was sequenced to identify the isolates, and the results showed 98–100% identity with comparable S. aureus from the NCBI database. The isolates were then placed in the NCBI GenBank and assigned the accession numbers from OP363093 to OP363122. Then, we screened ethanolic and methanolic extracts of Rhazya stricta leaves against clinical MRSA isolates to measure their growth inhibition property using MIC and disk diffusion methods. In addition, these extracts were used to measure the degree to which crystal violet inhibited biofilms. HPLC testing revealed 19 constituents including stilbene (resveratrol), quinol, 11 phenolic acids, and 6 flavonoids, and showed that there are differences between the extracts in the element’s number and their amounts. Both extracts showed antimicrobial properties against pathogenic microbes such as MRSA isolates. Extracts from R. stricta displayed potent inhibitory action on biofilms, with inhibition rates extending from 71.5% to 99% and from 26.2% to 98.9% for the ethanolic and methanolic extracts, respectively. R. stricta leaf extracts revealed potent antimicrobial as well as anti-biofilm activities of MRSA isolates and might be a distinct substitute for the prevention and therapy of the pathogen MRSA.

VIEWS 119

Wojtyczka RD, Dziedzic A, Kepa M, Kubina R, Kabała-Dzik A, Mularz T, Idzik D. 2014. Berberine enhances the antibacterial activity of selected antibiotics against coagulase-negative staphylococcus strains in vitro. Molecules 19, 6583-96.

Karygianni L, Ren Z, Koo H, Turnheer T. 2020. Bioflm matrixome: extracellular components in structured microbial communities. Trends Microbiol 28, 668-81.

Taglialegna A, Lasa I, Valle J. 2016. Amyloid structures as bioflm matrix scafolds. J Bacteriol 198, 2579-88.

Piechota M, Kot B, Frankowska-Maciejewska A, Grużewska A, Woźniak-Kosek A. 2018. Biofilm Formation by Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains from Hospitalized Patients in Poland. BioMed Res Int, 4657396.

Gilbert P, Maira-Litran T, McBain AJ, Rickard A, Whyte F. 2002. The physiology and collective recalcitrance of microbial biofilm communities. Adv Microb Physiol 46, 202-56.

Nassima B, Behidj N, Ksouri R. 2019. Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria). Braz J Pharm Sci, 55.

Macé S, Hansen L, Rupasinghe H. 2017. Anti-Bacterial Activity of Phenolic Compounds against Streptococcus pyogenes. Medicines 4, 25.

Miklasińska-Majdanik M, Kępa M, Wojtyczka RD, Idzik D, Wąsik TJ. 2018. Phenolic Compounds Diminish Antibiotic Resistance of Staphylococcus aureus Clinical Strains. Int J environ Res public health 15, 2321.

Albeshri A, Baeshen NA, Bouback TA, Aljaddawi AA. 2021. A Review of Rhazya stricta Decne Phytochemistry, Bioactivities, Pharmacological Activities, Toxicity, and Folkloric Medicinal Uses. Plants 10, 2508.

Baeshen MN, Khan R, Roop SB, Nabih AB. 2015. Therapeutic Potential of the Folkloric Medicinal Plant Rhazya stricta. Biol Syst 5, 1.

Bukhari NA, Al-Otaibi RA, Ibhrahim MM. 2017. Phytochemical and taxonomic evaluation of Rhazya stricta in Saudi Arabia. Saudi J Biol Sci 24, 1513-21.

Marwat SK, Usman K, Shah SS, Anwar N, Ullah I. 2012. A review of phytochemistry, bioactivities and ethno medicinal uses of Rhazya stricta Decsne (Apocynaceae). Afr J Microbiol Res 6, 1629-41.

Raziuddin K, Baeshen MN, Kulvinder SS, Nabih AB. 2018. Antibacterial activities of Rhazya stricta leaf extracts against multidrug-resistant human pathogens, Biotechnol Biotechnol Equip 30, 1016-25.

Sultana N, Khalid A. 2010. Phytochemical and enzyme inhibitory studies on indigenous medicinal plant Rhazya stricta. Nat Prod Res 24, 305-14.

Garoy EY, Gebreab YB, Achila OO, Tekeste DG, Tesfu T. 2019. Methicillin-Resistant Staphylococcus aureus (MRSA): Prevalence and Antimicrobial Sensitivity Pattern among Patients-A Multicenter Study in Asmara, Eritrea. Can J Infect Dis Med Microbiol 6, 8321834.

Alsanie WF. 2020. Molecular diversity and profile analysis of virulence-associated genes in some Klebsiella pneumoniae isolates. Pract Lab Med 19, e00152.

Alsanie WF, Felemban EM, Farid MA, Hassan, MM, Sabry A, Gaber A. 2018. Molecular identification and phylogenetic analysis of multidrug-resistant bacteria using 16S rDNA sequencing. J Pure Appl Microbiol 12, 489-96.

Andrews JM, Howe RA. 2011. BSAC standardized disc susceptibility testing method (version 10). J Antimicrob Chemother 66, 2726-57.

Matilla-Cuenca L, Gil C, Cuesta S, Rapún-Araiz M, Jaione V. 2020. Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids. Sci Rep 10, 18968.

Beigomi M, Shahraki-Mojahed L, Heydari-Sadegh B, Dahmardeh N, Rouhani R, Javadian F. 2021. Evaluation of Antimicrobial Activity of Rhazya Stricta (Apocynaceae) Extract Prepared with Different Solvents on Staphylococcus aureus (Staphylococcaceae) Isolated from Humans. Int J Adv Biol Biomed Res 9, 241- 53.

Xie Y, Yang W, Tang F, Chen X, Ren L. 2021. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Cur Med Chem 22, 132-49.

Bhawna T, Ashutosh D, Verma AK, Salil T. 2015. Antibacterial Activity of Phenolics compounds against Pathogenic Bacteria. Int J Pharm Sci Rev Res 35, 16-18.

Lagha R, Ben Abdallah F, Al-Sarhan B, Al-Sodany Y. 2019. Antibacterial and Biofilm Inhibitory Activity of Medicinal Plant Essential Oils Against Escherichia coli Isolated from UTI Patients. Molecules 24, 1161.

Bagamboula C, Uyttendaele M, Debevere J. 2004. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. J Food Microbiol 21, 33-42.

Qin R, Xiao K, Li B, Jiang W, Peng W, Zheng J, Zhou H. 2013. The combination of catechin and epicatechin gallate from Fructus Crataegi potentiates beta-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) in vitro and in vivo. Int J Mol Sci 14, 1802-21.

Oulkheir S, Aghrouch M, El Mourabit F, Dalha F, Graich H, Chadli S. 2017. Antibacterial Activity of Essential Oils Extracts from Cinnamon, Thyme, Clove and Geranium Against a Gram Negative and Gram-Positive Pathogenic Bacteria. J D M P 3, 1-5.

Chaieb K, Chehab O, Zmantar T, Rouabhia M, Mahdouani K, Bakhrouf A. 2007. In vitro effect of pH and ethanol on biofilm formation by clinical ica-positive Staphylococcus epidermidis strains. Ann Microbiol 57, 431-37.

Hassan MM, Albogami B, Mwabvu T, Awad M, Kadi R, Mohamed A, Al-Orabi J, Hassan MM, Elsharkawy M. 2023. The Antibacterial Activity of Rhazya stricta Extracts against Klebsiella pneumoniae Isolated from Some Soil Invertebrates at High Altitudes. Molecules 28, 3613.

Cushnie TP, Lamb AJ. 2011. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 38, 99-07.

Zhao Y, Chen M, Zhao Z, Yu S. 2015. The antibiotic activity and mechanisms of sugarcane (Saccharum officinarum L.) bagasse extract against food-borne pathogens. Food Chem 185, 112-18.

Kepa M, Miklasi´nska-Majdanik M, Wojtyczka RD, Idzik D, Wasik TJ. 2018. Antimicrobial potential of caffeic acid against Staphylococcus aureus clinical strains. Biomed Res Int 7413504.

Lima VN, Oliveira-Tintino CD, Santos ES, Morais LP, Tintino SR, Menezes IR. 2016. Antimicrobial and enhancement of the antibiotic activity by phenolic compounds: Gallic acid, caffeic acid and pyrogallol. Microb Pathog 99, 51-56.

Borges A, Ferreira C, Saavedra MJ, Simões M. 2013. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug 19, 256-65.

Xie Y, Chen J, Xiao A, Liu L. 2017. Antibacterial Activity of Polyphenols: Structure-Activity Relationship and Influence of Hyperglycemic Condition. Molecules 22, 1913.

Park KS, Chong Y, Kim MK. 2016. Biological activity related to human health. Appl Biol Chem 59, 259-69.

Ma C, He N, Zhao Y, Xia D, Wei J, Kang W. 2019. Antimicrobial Mechanism of Hydroquinone. Appl Biochem Biotechnol 189, 1291-03.

Griep MA, Blood S, Larson MA, Koepsell SA, Hinrichs SH. 2007. Myricetin inhibits Escherichia coli DnaB helicase but not primase. Bioorg Med Chem 15, 7203-08.

Zaixiang L, Hongxin W, Shengqi R, Juntao S, Chaoyang M, Jing L. 2012. p-Coumaric acid kills bacteria through dual damage mechanisms. Food Cont 25, 550-54.

Wang L, Bi C, Cai H, Liu B, Zhong X, Deng X, Wang T, Xiang H, Niu X, Wang D. 2015. The therapeutic effect of chlorogenic acid against Staphylococcus aureus infection through sortase A inhibition. Front Microbiol 6, 103.