J. Bio. Env. Sci.13(4), 131-140, October 2018
The present study determined the antibacterial activity of E. indica against E. coli and S. aureus at 12, 24 and 36 hrs of incubation. Also, its teratogenic potential using zebra fish as model animal was made. Malformations which include stunted-tail, growth retardation, yolk deformity and delayed development were observed. Similarly, mortality rate, hatchability and heartbeat of zebrafish embryo were also noted. Results showed bacterial inhibition against E. coli at 12, 24 and 36 hrs of incubation. For the teratogenicity of E. indica, LC50 of ethanol and hot water extracts was computed at 2200 ppm and 12890 ppm, respectively. Also, exposure of zebra fish embryo to ethanol extract of 10000 to 5000 ppm caused 100%mortality at 24 hours of incubation and reduced heartrates for the surviving embryos. Furthermore, malformations were also recorded which include stunted-tail, growth retardation, yolk deformity and delayed development. Thus, the potential antibacterial activity against E. coli and teratogenic potential of E. indica ethanol extracts.
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