Antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected gram negative and gram-positive bacteria

Paper Details

Research Paper 01/09/2019
Views (371) Download (14)
current_issue_feature_image
publication_file

Antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected gram negative and gram-positive bacteria

Enwa Felix Oghenemaro, Anie Clement Oliseloke, Iyamu Mercy Itohan, Eredajaye Omoriode Mary
Int. J. Biosci.15( 3), 48-54, September 2019.
Certificate: IJB 2019 [Generate Certificate]

Abstract

It is obvious from available literatures that not much has been done with respect to Tridax recumbence on its effects bacterial in South – south Nigeria, hence the need to investigate the antibacterial activities of the aqueous and methanol flower extract of Tridaxprocumbensagainst the following selected clinical isolates: Staphylococcus aureus, Lactobacillus and Shigella flexnerii. Theflower extract of Tridax recumbencewas obtainedusing cold maceration technique by weighing the powdered plant and macerated in distilled water and 70% methanol separately and allowed to stand at room temperature for a period of 4 days and 7 days respectively with frequent agitation. The antimicrobial assay done using agar well diffusion method and zones of inhibition for both methanol and aqueous extractand zones of inhibition recorded. The study shows that at a concentration of 300mg/ml of the methanol extract of Tridax procumbence on the test organisms; S. aureus, Lactobacillus and S. flexnerii, the zones of inhibition obtained were 8.0mm, 8.5mm and 6mm respectively. Lactobacillusand S. aureus showed an increase antibacterial effect when extract concentration was increased but S. flexneriishowed irregular zones inhibition with its value observed at 37.5mg/ml and 150mg/ml. The Minimum Inhibitory Concentration on the test organisms was 150mg/ml for S. aureus and 37.5mg/ml for Lactobacillus while S. flexnerii showed resistance to the extract at all the concentrations used. Tridaxprocumbence flower extract can be a good candidate for antibacterial agents and it is suggested that the flower extract could be a promising lead compound for the synthesis of effective chemotherapeutics.

VIEWS 16

Chitra Pai, Ujjwala Kulkarni, Manjusha Borderlands, Sowmya Murali P, Mrudala Yashwant Deshmukh. 2011.  Antibacterial activity of Tridaxprocumbens with special reference to nosocomial pathogens. British Journal of Pharmaceutical research 1(4), 164-173.

Collin H, Patricia NL. 1970. Complementary and Alternative Medicine, Microbiology methods, 3rd Edition, London University Press, Butterworth. Accessed at: July10, 2009. http://www.biomedcentral.com/1472-6882/5/6on

Mohale DS, Pokrna AR, Sanghani CV, Rasekar SR, rather AS, Mehetre GR, Chandewar AV. 2014. Antimicrobial activity of methanolic extracts of flowers of Tridaxprocumbens. Research Communication 1(1), 31-36.

Davies J. 1994. Inactivation of Antibiotics and the Dissemination of Resistant Genes. International Journal of Science 264, 375-382.

Elujoba AA. 1998. Pharmacognostical Standardization of Herbal Medicine: Standardization and Utilization of Herbal Medicine: Challenges of the 21st century.  Proceedings of the first International Workshop on Herbal Medicine Products, Nov. 22-24, Ibadan, Nigeria, p 30-43.

Falodun A, Imieje V. 2013.Herbal Medicine in Nigeria: Holistic Overview. Nigerian Journal of Science and Environment 12(1), 1-13.

Fettweis JM, Brooks JP, Seirano MG, Seth NU, Girerd PH, Edwards DJ, Strauss JF, Jefferson KK, Buck GA. 2014. Differences in vaginal microbiome in African American women versus women of European ancestry.  Microbiology 160(10), 2272-82.

Holm L, Doll J, Holm E, Pancho J, Herberger J. 1997. World Weeds. Natural Histories and Distribution. New York, USA; John Wiley and Sons, Inc.

Ikewuchi JC, Anyadiegwu A, Ugono EY, Okungbowa SO. 2008. Effect of Acalyphawilkesiana Muell. Arg. on Plasma Sodium and Potassium Concentration of Normal Rabbits. Pakistan Journal Nutrient 7(1), 130-132.

Kale M, Dhake A. 2013. Antibacterial potential of Tridaxprocumbens leaf extracts against some clinical pathogens. Journal of Natural Product and Plant Resource 3(6), 34-37.

Peterson LR, Dalhoff A. 2004.Screening for anti-infective properties of several medicinal plants of the Mauritians flora Journal of Antimicrobial Chemotherapy 53, 902-905.

Ma Bing Forney, Larry J, Ravel, Jacques. 2012. Vaginal Microbiome: Rethinking Health and Disease. Annual Review of Microbiology 66(1), 317-389.

Maiti Bodhisattva, Nagori BP, Rambir Singh, Pragati Kumar, Nishant Upadhyay. 2011. Recent Trends in Herbal Drugs: A Review: International Journal of Drug Research and Technology 1(1), 17-25.

Makarova K, Slesarev A, Wolf Y, Sorokin A Mirkin B, Koonin E, Pavlov A, Pavlova N. 2006. Comparative genomics of the lactic acid bacteria. Proceedings National Academy of Science of the United State America 103(42), 15611-6.

Masalha M, Borovok I, Schreiber R, Aharonowitz Y, Cohen G. 2001.  Analysis of transcription of the Staphylococcus aureus aerobic class Ib and anaerobic class III ribonucleotide reductase genes in response to oxygen. Journal of Bacteriology 183(24), 7260-72.

Osemene KP, Elujoba AA, Ilori MO. 2011. Comparative Assessment of Herbal and Orthodox Medicines in Nigeria. Research Journal of Medical Science 5(5), 34-41.

Petrova Mariya I, Lievensi Elke Malik, Shweta Imholz Nicole, Lebeer Sarah. 2015. Lactobacillus species as biomarkers and agents that can promote various aspects of vaginal health. Frontiers in Physiology. 6. ISSN 1664-042X. http://dx.doi.org/10.3389/fphys.2015.00081.

Ryan KJ, Ray CG, Sherris JC. 2004. Sherri’s Medical Microbiology (4th ed.) New York: Mc Graw-Hill. ISBN 978-0-8385-8529-0. LCCN 2003054. 180. OCLC 52358530.

Soerjani M, Kostermans AJGH, Tjitrosoepomo G. 1987. Weeds of Indonesia. Jakarta, Indonesia: Balai Pustaka, p 716.

Suffredini IB, Sarder HS, Goncalves AG. 2004. Screening of antibacterial extracts fromplants native to Brazillian amazon Rain Forest and Atlantic Forest. Brazillian Journal of Medical and Biological Research 37, 379-384.

Bharathi V, Varalakshmi B, Gomath S, Shanmuga Priya A, Karanam T. 2012. Antibacterial activity of Tridaxprocumbens Linn, International Journal of Pharmaceutical Sciences and Research 3(4), 364 – 367.

Wani Minal Pande, Snehal More Nitin. 2010.  “Callus induction studies in Tridaxprocumbens L. “.  International Journal of Biotechnology Applications 2(1), 11–4. http://dx.doi.org/10.9735/0975-2943.2.1.11-14

World Health Organization. 1991. Guidelines for the Assessment of Herbal Medicines. WHO/TRM/91.4. Geneva, Switzerland.