Antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected gram negative and gram-positive bacteria

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Research Paper 01/09/2019
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Antibacterial evaluation of aqueous and methanol extracts of Tridax procumbens flower on selected gram negative and gram-positive bacteria

Enwa Felix Oghenemaro, Anie Clement Oliseloke, Iyamu Mercy Itohan, Eredajaye Omoriode Mary
Int. J. Biosci.15( 3), 48-54, September 2019.
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It is obvious from available literatures that not much has been done with respect to Tridax recumbence on its effects bacterial in South – south Nigeria, hence the need to investigate the antibacterial activities of the aqueous and methanol flower extract of Tridaxprocumbensagainst the following selected clinical isolates: Staphylococcus aureus, Lactobacillus and Shigella flexnerii. Theflower extract of Tridax recumbencewas obtainedusing cold maceration technique by weighing the powdered plant and macerated in distilled water and 70% methanol separately and allowed to stand at room temperature for a period of 4 days and 7 days respectively with frequent agitation. The antimicrobial assay done using agar well diffusion method and zones of inhibition for both methanol and aqueous extractand zones of inhibition recorded. The study shows that at a concentration of 300mg/ml of the methanol extract of Tridax procumbence on the test organisms; S. aureus, Lactobacillus and S. flexnerii, the zones of inhibition obtained were 8.0mm, 8.5mm and 6mm respectively. Lactobacillusand S. aureus showed an increase antibacterial effect when extract concentration was increased but S. flexneriishowed irregular zones inhibition with its value observed at 37.5mg/ml and 150mg/ml. The Minimum Inhibitory Concentration on the test organisms was 150mg/ml for S. aureus and 37.5mg/ml for Lactobacillus while S. flexnerii showed resistance to the extract at all the concentrations used. Tridaxprocumbence flower extract can be a good candidate for antibacterial agents and it is suggested that the flower extract could be a promising lead compound for the synthesis of effective chemotherapeutics.


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