Antibacterial potential of methanolic extract of Syzygiumaromaticum (clove) against different bacterial strains

Paper Details

Research Paper 01/02/2020
Views (246) Download (17)
current_issue_feature_image
publication_file

Antibacterial potential of methanolic extract of Syzygiumaromaticum (clove) against different bacterial strains

Emad Mohamed Abdallah
Int. J. Biosci.16( 2), 394-401, February 2020.
Certificate: IJB 2020 [Generate Certificate]

Abstract

The flower buds of Syzygium aromaticum (Cloves) are a well-known spice, prescriped for treatment from some microbial diseases since ancient civilizations and in traditional medicine today. In the currenrt investigation, the methanolic extract of cloves was tested against 4 Gram-positive bacteria , namely Bacillus cereus ATCC10876, Staphylococcus saprophyticus ATCC 43867, Enterococcus faecalis ATCC 29212 and Staphylococcus epidermidis ATCC 12228. In addition to 5 strains  of Gram-negative bacteria , namely  Proteus vulgaris ATCC 6380, Klebsiella pneumoniae ATCC 27736, Salmonella typhimurium ATCC 14028, Shigella flexneri ATCC 12022 and  Escherichia coli ATCC 25922, using two diffusion methods (Cup-plate and disc diffusion methods), besides MIC and MBC testings. The extract showed noticable dose-dependant antibacterial activity against all tested bacterial strains with varied degrees, and there was no significant differences between the results of the cup-plate diffusion assay and the disc-diffusion assay. Moreover, MIC values were between 3.9 to 125 mg/ml, and MBC values ranged between 7.8 to 125 mg/ml, which was higher than the MIC’s, the MBC/MIC ratio indicating that the extract has a bactericidal attitude, which makes it suitable source for the formulation of new antibacterial drugs.

VIEWS 14

Abdallah EM. 2011. Plants: An alternative source for antimicrobials. Journal of Applied Pharmaceutical Science 1(6), 16-20.

Abdallah EM. 2016. Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacterbaumannii. Journal of Acute Disease 5(6), 512–516.

Abdallah EM, Elsharkawy ER. 2019. Antibacterial Activity of Ethyl Acetate Extract of Platycladusorientalis against Staphylococcus saprophyticus. Journal of Pure and Applied Microbiology 13(2), 1063-1068.

Akinyemi KO, Oluwa OK, Omomigbehin EO. 2006. Antimicrobial activity of crude extract of three medicinal plants used in South-West Nigeria folk medicine on some food borne bacterial pathogens. Afrin. Journal of Traditional and Complementary Medicine 3(4), 13-22. 11.

Amenu D. 2014. Antimicrobial Activity of Medicinal Plant Extracts and Their Synergistic Effect on Some Selected Pathogens. American j Ethnomed 1(1), 18-29.

Babu AD, Uma MRC. 2010. Antimicrobial activity of Methanol Extract of OxystelmaEsculentum. Journal of Pharmaceutical Science and Technology 2(2), 119-123.

Borris RP. 1996. Natural products research: perspectives from a major pharmaceutical company. Journal of Ethnopharmacology 51, 29-38.

Compean KL, Ynalvez RA. 2014. Antimicrobial Activity of Plant Secondary Metabolites: A Review. Res J Med Plants. 8(5), 204-213.

Cortés-Rojas DF, Souza CRF, Oliveira WP. 2014. Clove (Syzygiumaromaticum): a precious spice. Asian Pac J Trop Biomed 4(2), 90-96.

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiol Review 12(4), 564-582.

Doughari JH. 2006. Antimicrobial Activity of Tamarindusindica Linn. Tropical Journal of Pharmaceutical Research 5(2), 597-603.

Harold C, Neu HC. 1992. The Crisis in Antibiotic Resistance. Science 257(5073), 1064-1073.

Hemalatha R, Nivetha P, Mohanapriya C, Sharmila G, Muthukumaran C, Gopinath M. 2016. Phytochemical composition, GC-MS analysis, in vitro antioxidant and antibacterial potential of clove flower bud (Eugenia caryophyllus) methanolic extract. Journal of food science and technology 53(2), 1189–1198. http://dx.doi.org/10.1007/s13197-015-2108-5

Mittal M, Gupta N, Parashar P, Mehra V, Khatri M. 2014. Phytochemical evaluation and pharmacological activity of Syzygiumaromaticum: A comprehensive review. International Journal of Pharmaceutical Sciences 6(8), 67-72.Ll

Okmen G, Mammadhkanli M, Vurkun M. 2018. The antibacterial activities of Syzygiumaromaticum (L.) Merr. & Perry against oral bacteria and its antioxidant and ant mutagenic activities. International Journal of Pharmaceutical Sciences and Research 9(11), 4634-41. http://dx.doi.org/10.13040/IJPSR.09758232.9(11).4634-41.

Pathirana HNKS, Wimalasena SHMP, De Silva BCJ, Hossain S, Gang Joon H. 2019. Antibacterial activity of clove essential oil and eugenol against fish pathogenes (Paralichthysolivaceus). Slovenian Veterinary Research 56(1), 31–38.

Palhares RM, Drummond MG,  Brasil BSAF, Cosenza GP,  Brandão MGL,  Oliveira G. 2015. Medicinal Plants Recommended by the World Health Organization: DNA Barcode Identification Associated with Chemical Analyses Guarantees Their Quality. PLoS One. 10(5), e0127866.

Pandey A, Singh P. 2011. Antibacterial activity of Syzygiumaromaticum (clove) with metal ion effect against food borne pathogens.  Asian Journal of Plant Science and Research 1(2), 69-80.

Richardson LA. 2017. Understanding and overcoming antibiotic resistance. PLoS Biology 15(8), e2003775.

Zaman SZ,  Hussain MA, Rachel NyeMehta V, Mamun KT,   Hossain N. 2017. A Review on antibiotic resistance: alarm bells are ringing. Cureus. 9(6), e1403.

Richardson LA. 2017. Understanding and overcoming antibiotic resistance. PLoS Biology 15(8), e2003775.