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Antibiogram of gram-negative bacteria isolated from in-house patient samples in a tertiary care hospital in Kolkata

Asif Mahmud, Puja Kapuria, Pronob Sarkar

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Int. J. Biosci.15(3), 364-370, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.364-370


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Emerging resistance in Gram-negative bacteria and antibiotic use pattern are major concerns in clinical settings. This study aimed to detect common gram-negative bacteria and their antibiotic resistance patterns within 4 consecutive months in a tertiary care hospital in Kolkata. We determined the prevalence and antibiotic resistance patterns of bacterial isolates from blood, sputum, wound swab, urine and stool from February to May of 2015 at the adult ward in Fortis Hospital, Anandapur, and Kolkata. Identification of bacteria was done by the biochemical tests such as nitrate, indole, oxidase and urease. Antimicrobial resistance screening was performed by using 13 antibiotics, by the Kirby Bauer disk diffusion technique. E. coli was the most prevalent pathogen (38.35%) followed by Klebsiella (26.31%), Pseudomonas (21.05%), Acinetobacter (14.29%). 33.08% of the isolated gram negative organisms were found to be multidrug resistant. In our study MDR is high in E. coli (43.14%) followed by Pseudomonas (28.57%), Acinetobacter (26.31%), Klebsiella (25.71%). Antibiotic resistivity pattern of 7 different antibiotic groups were done in this study, in which most effective drug compared to other was found to be cephalosporins with an average of approx. 6.7% resistivity in all organisms. Overall, our findings indicate the prevalence of resistance to different classes of antibiotics in bacterial isolates from different infections and hence highlight the need for effective surveillance, regulator reporting, and antibiogram-guided antibiotic prescription.


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Antibiogram of gram-negative bacteria isolated from in-house patient samples in a tertiary care hospital in Kolkata

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