Welcome to International Network for Natural Sciences | INNSpub

Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita

Research Paper | January 1, 2018

| Download 9

Shameem Shabana, Mayavan Karthika, Ponnusamy Suganya, Venkatachalam Ramasubramanian

Key Words:

Int. J. Biosci.12( 1), 42-50, January 2018

DOI: http://dx.doi.org/10.12692/ijb/12.1.42-50


IJB 2018 [Generate Certificate]


The microbiological studies on freshwater fish culture appear to be limited predominantly in India. Consequently a study was done to identify the bacterial populations and pathogens in the sample water and in some tissues of cultured carp Labeo rohita. Among the various organs analyzed, the maximum bacterial load was found in gills followed by intestine and skin surface of the infected Labeo rohita. In general, the bacteria isolated from fish samples appeared to be very similar to those obtained from water. The Bacterial isolates were confirmed based on the morphological, Physiological and Biochemical Characteristics using standard methods. In biochemical test it is observed, that the bacteria was 90 percentage confirmed as Klebsiella genus which is gram negative and rod shape. For further confirmation, the bacterial sample have been inoculated with specific selective medium (Mac Conkey Agar Medium) and confirmed that the bacteria as Klebsiella pneumoniae by the appearance of the colony and change in the colour of the medium. Among the 13 antibiotic discs, certain antibiotics were sensitive such as Gentamicin-32mm, Ofloxacin-29mm, kanamicin-28mm and tetracycline-25mm against the pathogenic bacteria K. pneumoniae. This present study shows that Gentamicin, Ofloxacin, Kanamycin and Tetracycline can be used as a drug against the pathogenic bacteria K. pneumoniae for the infected fish (Labeo rohita).The above mentioned antibiotic disc against K. pneumoniae induced the growth of Labeo rohita and promote the aquaculture production and increase the economic status of aquaculture.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Antibiogram of Pathogenic bacteria isolated and identified from edible fish Labeo rohita

Archana Singh Sikarwar, Harsh Vardhan Batra. 2011. Prevalence of Antimicrobial Drug Resistance of Klebsiella pneumoniae in India, International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 1, No. 3, http://dx.doi.org/10.7763/IJBBB.2011.V1.38.

Ayeloja AA, George FOA, Obasa SO, Sanni LO. 2011. Effect of Posts laughter Time Intervals on the Quality of the African Catfish, Clarias gariepinus (Burchell, 1822). American Journal of Food Technology 6(9): 790-797. ISSN 1557- 4571/ http://dx.doi.org/10.3923/ajft.2011.790.797.

Bauer AW, Kirby WMM, Sherris JC,Turck M. 1966. Antibiotic susceptibility testing by a standardized disk method. American Journal of Clinical Pathology, 45(4), 493-496.

Cann DC, Taylor LY. 1982. An outbreak of botulism in rainbow trout, Salmo gairdneri Richardson, farmed in Britain. Journal of Fish Diseases, Volume no. 5:393-399, http://dx.doi.org/10.1111/j.13652761.1982.tb00495.x.

Diana TC, Ramulu KS. 2010. Changes in the protein content of liver and intestine in  relation to Klebsiella infection in Nemipterus japonicus from the coast of Visakhapatnam, India. The Asian Journal of Animal Science. 4(2), 143-145.

Edwards P. 2013. Peter Edwards writes on rural aquaculture. Pond aquaculture is taking off in Nepal. Aquaculture  Asia, Volume XVIII No. 4.

FAO. 1987. Site selection for aquaculture: chemical features of water, United Nations Development Programme Food and Agriculture Organization of the Unitednations Nigerian institute for Oceanography and Marine Research Project raf/82/009.

FAO. 2012. The state of world fisheries and aquaculture, World review of fisheries and aquaculture. www.FAO.org/docrep/016/i2727e/i2727e/i2727e01

FAO. 2013. Food and Agriculture Organization of the United Nations: The State of World Fisheries and Aquaculture, (Rome).

FAO. 2014. The state of world fisheries and aquaculture. Food and Agricultural Organizations of the United States. http://www.fao.org/3/a-i3720e.pdf

Kühlwein H, Merrifield DL, Rawling MD, Foey AD, Davies SJ. 2014. Effects of dietary β-(1,3) (1,6)-D-glucan supplementation on growth performance, intestinal   morphology and haemato-immunological profile of mirror carp (Cyprinus carpio L.). Journal of Animal Physiology and Animal Nutrition, 98(2), 279–289, http://dx.doi.org/10.1111/jpn.12078.

Leung TLF, Bates AE. 2013. More rapid and severe disease outbreaks for aquaculture at the tropics: implications for food security. Journal of Applied Ecology 50(1), 215–222, http://dx.doi.org/10.1111/1365-2644.12017.

Mohapatra S, Chakraborty T, Prusty AK, Das P, Paniprasad K, Mohanta KN. 2012. Use of different microbial probiotics in the diet of Rohu, Labeo rohita   fingerlings: effects on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal microflora. Aquaculture Nutrition 18, 1–11, http://dx.doi.org/10.1111/j.1365-2095.2011.00866.x.

Ogbonna DN, Sokari TG, Amaku GE. 2008. Antibiogram of bacterial flora of Tilapia zilli from creeks around port harcourt, Nigeria. Journal of Enivornmental Science and Technology 1, 27- 33, http://dx.doi.org/10.3923/jest.2008.27.33.

RAMP. 2016. Water quality indicators: Conventional variables regional aquatics monitoring program pp 1-2.

Romero J, Feijoo CG, Navarrete P. 2012. Antibiotics in Aquaculture – Use, Abuse and Alternatives. INTECH Open Access, 159–198, http://dx.doi.org/10.5772/28157.

Saraswathi R, Sumithra P, Sivakami R. 2015. Identification of Enterobacteriaceae studies in Carps during rearing a fresh water pond, International Journal of Current Microbiology and Applied Sciences, ISSN: 2319-7706 Volume 4 Number 12,  661-669 p.

Sharma M, Shrivastav AB, Sahni YP, Pandey G. 2012. Overviews of the    treatment and control of common fish diseases, International Research Journal of Pharmacy, ISSN 2230-8407, 3(7), 123-127.

Sivakami R, Sankar Rao M, Shimna PP and Premkishore G.  2011. Occurrence of airborne mycoflora spores at Uyyakkondan channel in river Cauvery, Tiruchirappalli, Tamil Nadu. Journal of Current Science, 16: 183- 186.

Subramanian S, Mackinnon SL, Ross NW. 2007. A comparative study on innate immune parameters in the epidermal mucus of various fish species. Comparative Biochemistry and Physiology, 148(3), 256-263, https://doi.org/10.1016/j.cbpb.2007.06.003

Taylor SL. 1986. Histamine food poisoning: toxicology and clinical aspects. CRC Critical Reviews in Toxicology. 17(2), 91–128.

Vadstein O, Bergh O, Gatesoupe FJ, Galindo-Villegas J, MuleroV, Picchietti S, Scapigliati G, Makridis P, Olsen Y, Dierckens K, Defoirdt T, Boon N, Schryver PD, Bossier P. 2012. Microbiology and immunology of fish larvae. Rev. Aquaculture, 5, 1–25.