Antibiotic susceptibility and molecular detection of MEXT gene of Pseudomonas aeruginosa isolated from burned patients

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Research Paper 01/09/2018
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Antibiotic susceptibility and molecular detection of MEXT gene of Pseudomonas aeruginosa isolated from burned patients

Sidra Anam, Faisal Rasheed Anjum, Tayyaba Younas, Sajjad ur Rahman, Muhammad Usman
Int. J. Biosci.13( 3), 276-282, September 2018.
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Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic human pathogen and a leading cause of disease and death in burned patients. Nosocomial infections caused by P. aeruginosa are increasing worldwide that can be attributed to uncontrolled use of antibiotics in hospitals and community. The present study was aimed to decipher the antibiotic susceptibility and molecular detection of MEXT gene in multidrug resistant P. aeruginosa isolates from burned patients. A total of 100 swab samples from burned patients were collected and cultured on cetrimide agar plates followed by enrichment in nutrient broth. Bio characterization was done for positive pigment producing isolates. Antibiotic susceptibility was assessed by Kirby Bauer disk diffusion method to commonly used antibiotics; amikacin, tobramycin, ciprofloxacin, colistin, carbenicillin, meropenem, and ceftazidim. The MEXT gene was amplified in multidrug resistant (MDR) isolates. It was found that 44% isolates were positive for P. aeruginosa giving pigment production and positive citrate and oxidase tests. Antibiogram results revealed that 56.8% isolates (13/44) were multidrug resistant. The MEXT gene in selected MDR isolates was detected with 216bp size. In conclusion, colistin and meropenem could be effective in treating P. aeruginosa infections and MEXT gene modulates the induction of multidrug efflux system that further modulates antibiotic resistance to diverse range of antibiotics in P. aeruginosa.


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