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Antibiotic treatment of citrus budwood for the management of huanglongbing disease

Muhammad Sarwar Yaqub, Iqrar Ahmad Khan, Rozina Aslam

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Int. J. Biosci.15(1), 99-109, July 2019

DOI: http://dx.doi.org/10.12692/ijb/15.1.99-109


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Control of huanglongbing (HLB) disease is necessary around the world to avoid complete destruction of citrus. Antibiotics were used by many scientists in the form of sprays and trunk injections to control HLB, but the control was not effective for longer time and disease symptoms appeared again after some time because of uneven distribution of pathogen in the host plant. Tetracycline and penicillin antibiotics were used in this study to eliminate Candidatus Liberibacter bacterium from sweet orangebudwood with the objective that the pathogen elimination from the budwood could be comparatively more effective. Tetracycline doses used were 250ppm, 500ppm, 750ppm, 1000ppm and 1250ppm. Penicillin doses were 25ppm, 50ppm, 75ppm, 100ppm and 125ppm. Kinnow mandarin (Citrus reticulata Blanco) nurtured in screen house were used as indicator plant. These plants were confirmedHLB negative before grafting. Budwood was obtained from HLB positive plants confirmed by conventional PCR using 16S rDNA primers OI1/OI2C and rplKAJL-rpoBC operon primers A2/J5. Budsticks treated with the above mentioned doses of antibiotics were grafted on the indicator plants. Tetracycline at 1000 ppm with mean Ct value of 38.40 and penicillin antibiotic at 75ppm dose with mean Ct value of 38.61 were found effective to eliminate Candidatus Liberibacter asiaticus from budwood to stop further spread of the disease through budding/grafting.


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Antibiotic treatment of citrus budwood for the management of huanglongbing disease

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