Anticancer activity of propolis flavonoid pinobanksin-3-acetate against human colon cancer in vitro

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Research Paper 01/08/2015
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Anticancer activity of propolis flavonoid pinobanksin-3-acetate against human colon cancer in vitro

Mutallip Amet, Abulizi Abudula, Abiban Akela, Lei Sheng, Buweihailiqiemu Ababaikeri, Amina Maimaiti, Yasuyuki Takigchi, Tatsuaki Yamaguchi, Yimit Rahman
Int. J. Biosci.7( 2), 45-55, August 2015.
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Abstract

Pinobanksin-3-acetate (PB3A) is a natural flavonoid commonly present in propolis, honey, and many plants. In this study, we evaluate the anticancer activity of PB3A on human colon cancer in vitro, and to elucidate the mechanism of its activity. Cells were tested in vitro for cytotoxicity, apoptotic peaks and gene expression profiling after treatment with PB3A, in which conducted cell proliferation assay, Flow cytometric (FCM) method and Human Genome Gene chip Array U133_Plus_2 analysis, respectively. PB3A exerted a significant inhibitory effect on SW480 cell proliferation and in a time- and dose-dependent manner, and PB3A could significantly increase the apoptosis rate in a dose-dependent manner. The gene profiling analysis identified that PB3A intervention induced a wide range of changes in the gene expression profile of SW480 cells. The findings in this study suggested that PB3A demonstrated potential anticancer activity on colon cancer in vitro, and it could induce apoptosis; which could be attributed, in part, to its inhibition of proliferation and induction of apoptosis in cancer cells through up-regulation and down-regulation of multiple genes involved in cell apoptosis, cytokinetics, colorectal carcinogenesis, Wnt, and Calcium signaling.

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