Antifungal activity and preliminary phytochemical analysis of bark extracts of Moringa oleifera Lam.

Paper Details

Research Paper 01/12/2012
Views (270) Download (5)
current_issue_feature_image
publication_file

Antifungal activity and preliminary phytochemical analysis of bark extracts of Moringa oleifera Lam.

Mudasser Zaffer, Showkat Ahmad, Rajendra Sharma, Surabhi Mahajan, Ankur Gupta, Rajneesh Agnihotri
Int. J. Biosci.2( 12), 26-30, December 2012.
Certificate: IJB 2012 [Generate Certificate]

Abstract

The Chloroform, Ethyl acetate, Methanol and aqueous extracts of Moringa oleifera bark traditionally used in folkloric medicine for the treatment of various infections were investigated for their antifungal activity against 2 fungal strains viz., Rizopus stolonifer and Microsporum gypseum by disc diffusion method. The different extracts of the plant species reduced colony growth of both the fungi to a great degree compared with the control treatment. The test pathogens differed considerably with regard to their susceptibility to plant extracts. Microsporum gypseum was the most susceptible being inhibited by most of the extracts. Methanol was found to be the best extractive solvent for antifungal activity in the test plant. The preliminary phytochemical analysis of Moringa oleifera bark extract revealed the presence of alkaloids, flavanoids, phenols, tannins, saponins and steroids. The results provide justification for the use of the plant in folk medicine to treat various infectious diseases.

VIEWS 6

Ahmad S, Sharma R, Mahajan S, Agnihotri R, Gupta A. 2012. Antibacterial evaluation and preliminary phytochemical analysis of leaf extract of Holoptelea integrifolia. J. Phar. Res. 5(7), 3823-3825.

Bonjar GHS, Nik AK, Aghighi S. 2004. Antibacterial and antifungal survey in plants used in indigenous herbal-medicine of south east regions of Iran. J. Biol. Sci. 4, 405-412.

Boyd I, Beveridge EG. 1981. Antimicrobial activity of some alkyl esters of gallic acid (3, 4, 5-trihydrobenzoic acid) against E. coli NCTC 5933 with particular reference to n-propil gallate. Microbios 30, 73-85.

Davis J. 1994. Inactivation of antibiotics and the dissemination resistance genes. Science. 264, 375-382

Harborne JB. 2005. Phytochemical Methods. Springer (India) Pvt. Ltd., New Delhi 17.

Haung X, Xie WJ, Gong ZZ. 2000. Characterization and antifungal activity of a chitin binding protein from Ginkgo biloba. FEBS. Lett. 478, 123-126.

Ibrahim MB. 1997. Anti-microbial effects of extract leaf, stem and root bark of Anogeissus leiocarpus on Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Proteus vulgaris. J. Pharma. Devpt 2, 20-30.

Kohner PC, Rosenblatt JE, Cockerill FR.1994. Comparison of agar dilution, broth dilution and disk diffusion testing of Ampicillin against Haemophilus spp. by using in house and commercially prepared media J. Clin. Microbiol. 32, 1594 -96.

Marjorie MC. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12, 564-582.

Ogundipe O, Akinbiyi O, Moody JO.1998. Antibacterial activities of essential ornamental plants. Nigeria J. Natural Products & Medicine 2, 46-47.

Raman N, 2006. Phtochemical Technique. New Indian Publishing Agencies, New Delhi. 19.

Wagner H, Bladt S. 1996. Drug Analysis.