Welcome to International Network for Natural Sciences | INNSpub

Antifungal activity of Pseudomonas strains isolated from wheat Rhizosphere against Fusarium sp.

Research Paper | May 1, 2017

| Download 8

Ferdaouss El Habil-Addas, Saida Aarab, Chaimae El Aaraj, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

Key Words:

Int. J. Biosci.10( 5), 207-215, May 2017

DOI: http://dx.doi.org/10.12692/ijb/10.5.207-215


IJB 2017 [Generate Certificate]


Fusarium diseases of small grain cereals, especially wheat cause significant yield losses worldwide. In this study, the antagonistic effect of Pseudomonas isolated from wheat rhizosphere, was studied against Fusarium species. The solubilization activity of mineral phosphate was evaluated using Pikovskaya’s (PVK) medium, PGP traits were checked and antagonistic activity was reached by dual culture technique in Potato Dextrose Agar (PDA) medium. A total of 71 Pseudomonas were isolated from the rhizosphere of tree varieties of wheat (Salama, Wafia and Rajae) cultivated in the Northwest of Morocco. Of which 52% were able to solubilize tri-calcium phosphate (TCP). On the basis of the (Solubilization index ≥ 1.44), 11 strains of Pseudomonas were screened for their plant growth promoting (PGP) traits and evaluated for the ability to suppress growth of Fusarium species. The selected bacteria were able to produce hydrogen cyanide (HCN) except PS11 and PR23. Only PS11 and PR9 isolates showed the production of siderophores. Production of indole acetic acid was observed only in two bacteria, PW9 and PR9. All the isolates were positive for the production of some researched hydrolytic enzymes (amylase, protease, cellulase and chitinase). Results showed that PR19 had maximum inhibition against Fusarium sp. (59.16±1.44%) whereas PW11 showed the least inhibitory effect (20.43±1.86%). These results make some Pseudomonas strains, as PR9 and PR19, attractive as PGP bacteria. However, it requires further studies under pot culture as well as field conditions before to be recommended as biofertilizers and biocontrol agents for wheat.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Antifungal activity of Pseudomonas strains isolated from wheat Rhizosphere against Fusarium sp.

Ahmad F, Ahmad I, Khan MS. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research 163, 173-81. http://dx.doi.org/10.1016/j.micres.2006.04.001

Aktuganov GE, Galimzyanova NF, Melent’ Ev AI, Kuz’ Mina LY. 2007. Extracellular hydrolases of strain Bacillus sp.739 and their involvement in the lysis of micromycetecell walls. Mikrobiologiia 76(4), 471–479. http://dx.doi.org/10.1134/S0026261707040054

Ames BN. 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods in Enzymology 8, 115-118. https://doi.org/10.1016/0076-6879(66)08014-5

Ashrafuzzaman M, Hossen FA, Ismail MR, Hoque MA, Islam ZM, Shahidullah SM, Meon S. 2009. Efficiency  of  plant  growth-promoting  rhizobacteria  (PGPR)  for  the  enhancement  of  rice growth. African Journal of Biotechnology 8(7), 1247-1252. http://dx.doi.org/10.5897/AJB2009.000-9198

Bakker AW, Schippers B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation.  Soil Biology and Biochemistry 19, 451-457. https://doi.org/10.1016/0038-0717(87)90037-X

Bogas AC, Watanabe MAE, Barbosa A, Vilas-Boas LA, Bonatto AC, Dekker R, Souza EM, Fungaro MHP. 2007. Structural characterization of the bglH gene encoding a beta glucosidase-like enzyme in an endophytic Bacillus pumilus strain. Genetics and Molecular Biology 30(1), 100–104. http://dx.doi.org/10.1590/S141547572007000100018 

Chernin L, Ismailov Z, Haran S, Chet I. 1995. Chitinolytic Enterobacter agglomeran santagonistic to fungal plant pathogens. Applied and Environmental Microbiology 61(5), 1720–1726. http://dx.doi.org/0099-2240/95/$04.0010

Chernin L, Chet I. 2002.Microbial enzymes in biocontrol of plant pathogens and pests. R.G. Burns, R.P. Dick, Eds., Enzymes in the environment: activity, ecology, and applications. Marcel Dekker, inc., New York; 171–225 p. http://dx.doi.org/10.1201/9780203904039.ch7

Costa JM, Loper JE. 1994. Characterization of siderophore production by the biological control agent Enterobacter cloacae. Molecular Plant-Microbe Interactions 7(4), 440–448. http://dx.doi.org/10.1094/MPMI-7-0440

Dowling DN, O’Gara F. 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends in Biotechnology 12(4), 133–141. http://dx.doi.org/10.1016/0167-7799(94)90091-4

Edi-Premono M, Moawad MA, Vleck PLG 1996.Effect of phosphate solubilizing Pseudmonas putida on the growth of maize and its survival in the rhizosphere. Indonesian journal of crop science 11, 13-23. http://dx.doi.org/10.4067/S071895162013005000001

El Aaraj C, Bakkali M, Infantino A, Arakrak A, Laglaoui A. 2015. Mycotoxigenic fungi in cereals grains and coffee from the North of Morocco. American Journal of Research Communication 3(2), 130-142.

Gordon SA, Weber RP. 1951. Colorimetric estimation of indole acetic acid. Plant Physiology 26(1), 192-195. http://dx.doi.org/10.1104/pp.26.1.192

Haas H, Défago G. 2005. Biological control of soil-borne pathogens by fluorescent Pseudomonas. Nature Reviews Microbiology 3(4), 307-319. http://dx.doi.org/10.1038/nrmicro1129

Hoster F, Schmitz JE, Daniel R. 2005. Enrichment of chitinolytic microorganisms isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain.  Applied Microbiology and Biotechnology 66, 434–442. http://dx.doi.org/10.1007/s00253-004-1664-9

Huang CJ, Wang TK, Chung SC, Chen CY. 2004.  Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28–9. Journal of Biochemistry and Molecular Biology 38(1), 82–88. http://dx.doi.org/10.5483/BMBRep.2005.38.1.082

Joseph B, Patra RR, Lawrence R. 2007. Characterization of plant growth promoting rhizobacteria associated with chickpea (Cicer arietinum L.). International Journal of Plant Production2, 141-152. http://dx.doi.org/10.22069/ijpp.2012.532

Kammoun R, Naili B, Bejar S. 2008. Application of a statistical design to the optimization of parameters and culture medium for α-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product). Bio resource Technology 99(13), 5602–5609. http://dx.doi.org/10.1016/j.biortech.2007.10.045

Killani AS, Abaidoo RC, Akintokun AK, Abiala MA. 2011. Antagonistic effect of indigenous Bacillus subtilis on root-/soil-borne fungal pathogens of cowpea. Researcher 3(3), 11-18. http://hdl.handle.net/123456789/9404

King EO, Ward MK, Randey DE. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44(2), 301-307.

Lee YJ, Kim BK, Lee BH, Jo KI, Lee NK, Chung CH, Lee YC, Lee JW. 2008. Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresource Technology 99(2), 378–386. http://dx.doi.org/10.1016/j.biortech.2006.12.013

Lim HS, Kim YS, Kim SD. 1991.Pseudomonas stutzeriYPL-1 genetic transformation and antifungal mechanism against Fusarium solani, an agent of plant root rot.  Applied and Environmental Microbiology 57, 510–516.

Logrieco A, Mulè G, Moretti A, Bottalico. 2002. Toxigenic Fusarium species and mycotoxins associated with maize Ear Rot in Europe. European Journal of Plant Pathology 108, 597–609. http://dx.doi.org/10.1023/A:1020679029993

Mahanta N, Gupta A, Khare SK. 2008. Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresource  Technology 99(6), 1729–1735. http://dx.doi.org/10.1016/j.biortech.2007.03.046

Mayak S, Tirosh T, Glick BR. 2004. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiology and Biochemistry 42(6), 565-572. http://dx.doi.org/10.1016/j.plaphy.2004.05.009

Munkvold GP. 2003. Epidemiology of Fusarium diseases and their mycotoxins in maize ears. European Journal of Plant Pathology 109, 705–713. http://dx.doi.org/10.1007/978-94-017-1452-5_5

Naureen Z, Yasmin S, Hameed S, Malik KA, Hafeez FY. 2005. Characterization and screening of bacteria from rhizosphere of maize grown in Indonesian and Pakistani soil. Journal of Basic Microbiology 45(6), 447-459. http://dx.doi.org/10.1002/jobm.200510566

Naureen Z, Price AH, Hafeez FY, Roberts MR. 2009. Identification of rice blast disease-suppressing bacterial strains from the rhizosphere of rice grown in Pakistan. Crop Proection 28(12), 1052-1060. http://dx.doi.org/10.1016/j.cropro.2009.08.007

Nelson PE, Toussin TA, Marassas WFO. 1983. Fusarium Species. An illustrated Manual for Identification. University Park Pennsylvania: Pennsylvania State University Press.

NganjeWE, Bangsund DA, LeistritzFL, Wilson WW, Tiapo NM. 2004. Regional economic impacts of Fusarium Head Blight in wheat and barley. Applied Economic Perspectives and Policy 26, 332–347. http://dx.doi.org/10.1111/j.1467-9353.2004.00183.x

Pikovskaya RI. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya 17, 362-370. 

Pitt JI, Hocking AD. 1997. Primary keys and miscellaneous fungi. Fungi and Food Spoilage.2nd Edition. Blackie Academic and Professional. London, Weinheim, New York, Tokyo, Melbourne, Madras, 59–171.http://dx.doi.org/10.1007/978-1-4615-6391-4_5

Placinta C, D’Mello JP, Macdonald AM. 1999. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology 78, 21–37. http://dx.doi.org/10.1016/S0377-8401(98)00278-8

Rachid D, Ahmed B. 2005. Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens. African Journal of Biotechnology 4(7), 697-702. http://dx.doi.org/10.5897/AJB2005.000-3129

Rangeshwaran R, Prasad RD. 2000. Biological controls of Sclerotium rot of sunflower.  Indian phytopathology 53(4), 444-449.

Roberts WK, Selitrinnikoff CP. 1988. Plant and Bacterial Chitinases Differ in Antifungal Activity. Journal of general microbiology 134, 169-176. http://dx.doi.org/10.1099/00221287-134-1-169

Ryu R, Patten CL. 2008.  Aromatic amino acid-dependent expression of indole-3-pyruvate decarboxylase is regulated by TyrR in Enterobacter cloacae UW5. Journal of Bacteriology 190(21), 7200-7208. http://dx.doi.org/10.1128/JB.00804-08

Saharan BS, Nehra V. 2011.  Plant growth promoting rhizobacteria: A critical review. Life Sciences and Medicine Research21, 1-30.

Samuel S, Muthukkaruppan SM. 2011. Characterization of plant growth promoting rhizobacteria and fungi associated with rice, mangrove and effluent contaminated soil. Current Botany2(3), 22-25.

Schwyn B, Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophore.  Analytical Biochemistry 160(1), 47-56. https://doi.org/10.1016/0003-2697(87)90612-9

Singh PP, Shin YC, Park CS, Chung YR. 1999. Biological control of Fusarium wilts of cucumber by chitinolytic bacteria. Phytopathology 89(1), 92–99. http://dx.doi.org/10.1094/PHYTO.1999.89.1.92

Tsai SH, Liu CP, Yang SS. 2007. Microbial conversion of food wastes for biofertilizer production with thermophiliclipolytic microbes. Renewable Energy 32, 904–915. http://dx.doi.org/10.1016/j.renene.2006.04.019

Toyoda H,Utsumi R. 1991. Method for the prevention of Fusarium diseases and microorganisms used for the same. U.S. Patent 4, 988, 586.

Van der Lee T, Zhang H, van Diepeningen A, Waalwijk C. 2015. Biogeography of Fusarium graminearum species complex and chemotypes: A review. Food Additives and Contaminants: PartA 32, 453–460. http://dx.doi.org/10.1080/19440049.2014.984244

Wahyudi AT, Astuti RP, Widyawati A, Meryandini AA, Nawangsih AA. 2011. Characterization of Bacillus sp.  strains  isolated  from  rhizosphere of  soybean  plants  for  their  use  as potential plant growth for promoting rhizobacteria. Journal of Microbiology and Antimicrobials 3(2), 34-40.

Yadav J, Verma JP, Tiwari KN. 2010. Effect of plant growth promoting Rhizobacteria on seed germination and plant growth Chickpea (Cicer arietinum L.) under in vitro conditions. Biological Forum-An International Journal 2(2), 15–18.