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Antifungal activity of some plant extracts and Trichoderma spp. against cucumber damping off caused by Pythium aphanidermatum

Research Paper | June 1, 2018

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Musa Khalaf Al-shemmary, Abdelnaser Abdelghany El-Zaawely, Azher Hameed Al-Taie, Nabil Ibrahem Elsheery

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J. Bio. Env. Sci.12( 6), 195-203, June 2018


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The present study aimed to use some plant extracts and two different species of Trichoderma spp. to control damping off disease in cucumber caused by Pythium aphanidermatum instead of using chemicals where serious environmental problems caused by several chemical fungicides. Some plant products such as plant metabolites and plant based pesticides considered to be better alternatives as they are known to have environmental impact as opposed to chemical pesticide. In the antagonistic assay that carried out with Trichoderma spp., the results revealed high inhibition on the tested pathogen, but in an assay of plant extracts somerplant extracts with different concentrations were tested in vitro for their activity against P. aphanidermatum. Some of the tested plant extracts significantly reduced the radial growth of the pathogen. Cinnamon, carnation and camphor were the most affective from all the eight tested plant extracts than the other extracts, where with the concentration of 30.000ppm Cinnamon, Carnation and Camphor gave the highest inhibition values against P. aphanidermatum, while mango and mint extracts did not give any inhibition for the radial growth of P. aphanidermatum.


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Antifungal activity of some plant extracts and Trichoderma spp. against cucumber damping off caused by Pythium aphanidermatum

Abd-El-Moiety TH, Abou-Zeid NM. 1985. Studies on the control of Botrytis fabae on faba bean in Egypt. Proceeding of the 1st. National Conference of Pests and Disease of Vegetable and Field Crops in Egypt, (PDVFCE’85) Ismailia pp: 779-790.

Al-Waely DSA. 2004. Study on damping off disease of Tomato and its integrate pest management (IPM) in Al-Zubair and Safwan farms. Ph. D thesis, Faculty of Scienc University of Basrah. P 110.

Bajwa R, Khalid A, Cheema TS. 2003. Antifungal activity of allelopathic plant extracts III: Growth response of some pathogenic fungi to aqueous extracts of Parthenium hysterophorus. Pakistan J. Pl. Pathol 2, 145–56.

Batish DR, Singh HP, Kohli RK, Kaur S. 2000. Eucalyptus essential oil as natural pesticide. Forest Ecology and Management 256, 2166- 2174.

Belete E, Ayalew A, Ahmed S. 2015. Evaluation of local Isolates of Trichoderma spp. against black root rot (Fusarium solani) on Faba bean. Journal of Plant Pathology and Microbiology 6, 279. 5-45. In French.

Christy Jeyaseelan E, Tharmila S, Niranjan K. 2012. Antagonistic activity of Trichoderma spp. and Bacillus spp. against Pythium aphanidermatum isolated from tomato damping off. Arch. Appl. Sci. Res 4(4), 1623 – 1627.

Decal A, Pascual S, Melgareio P. 1997. Infectivity of Chlamydospores vs Microconidia of Fusarium oxysporum f. sp., Lycopersici on Tomato. J. Phytopathology 145, 233 – 235.

Dewan MM. 1989. Identity and frequency of occurrence of fungi in roots of wheat and rye grass and their effection take all and host growth. Ph. D Thesis, Univ. Wes. Ausrtalia 210 p.

Domsch KH, Gams W, Anderson I. 1980. Compendium of soil fungi. Vol. 1. Academic Publishers.

El-Mougy NS, El-Gamal NG, Abdel-Kader MM. 2007. Control of wilt and root rot incidence in Phaseolus vulgaris L. by some volatile compounds. J Plant Prot Res 47(3), 255– 265.

Eman SH, Farrag, Moustafa HA, Moharam and El-Sayed H. Ziedan. 2013. Effect of plant extracts on morphological and pathological potential of seed-borne fungi on cucumber seeds. Jor. Of Agricultural Technology Vol. 9(1), 141 – 14.

Grieve M. 1992. In A modern herbal, Tiger Books International London. Dorset Press, 287 p.

Harman GE. 2000. Myths and dogmas of biocontrol change in perceptions derived from research on Trichoderma harzinum T. 22. Plant Dis Rep 84(4), 377- 393.

Jebir, Salman K. 2001. A survey of the disease of root rot and the bases and stems of faba bean and the diagnosis of causing fungi and its biological control. Jor. of Iraqi Agricultural Sciences 32(2), 127 – 132.

Kareem TA, Al-heety AA, Al-Karbooly HH. 2009. Effect of the oil of carnation on the control of Pythium aphanidermatum causes damping off disease on cucumber Diyala Journal of Agricultural Sciences 1(1), 248 – 263.

Killani AS, Abaidoo RS, Akintokun AK. 2011. Rice husk extract is potentially effective as a phytopesticide against root/soil-borne fungal pathogens of cowpea. Nature and Science 9(3).

Mansour RT, Aboulghar MA, Serour GI et al. 1996. Pregnancy and delivery after intracytoplasmatic injection of spermatids into human oocytes. Middle East Fertil. Soc. J 1.

Messiaen CM, Blancard D, Rouxel F, Lafon R. 1995. Enfermedades de lashortalizas. Ed MundiPrensa, Madrid. 576 pp. [In Spanish].

Omar MR, El-Samawaty AMA, El-Wakil DA. 2007. Suppression of Pythium ultimum involved in cotton seedling damping-off by Trichoderma spp. Egyptian Journal of Phytopathology 35(2), 111-124.

Quarles W. 2011. Compost tea for organic farming and gardening. The IPM Practitioner 23(9), 1-8.

Sahayaraj K, Borgio JF, Raju G. 2009. Antifungal activity of three fern extract on causative agents of groundnut early leaf spot and rust diseases. Journal Plant Protection 49(2), 141-144.

Salih MR, Ismael JHS. 2016. Integrated Management of Tomato Damping-off Disease in Bekrajo, Sulaimani Governorate, Kurdistan Region, Iraq.

SAS. 2012. Statistical Analysis System, User’s Guide. Statistical. Version 9.1th Ed. SAS. Inst. Inc. Cary. N.C. USA.

Van der Plaats-Niterink AJ van der. 1981. Monograph of the genus Pythium. Studies. Mycology central burcau voor- Schimmcleultures, Baarn, Netherlands (21) p 241.

Wang YH, Joobeur T, Dean RA, Staub JE. 2007. Cucurbit. Genome Mapping and Molecular Breeding in Plants 5: Vegetables.

Yedidia I, Srivastva AK, Kapulnik Y, Chet I. 2001. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil 235, 235-42.

Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. 2011. Application of biofungicides in control of damping off disease in greenhouse crops as a possible substitute to synthetic fungicides, Int. Jor. Environ. Sci. Tech 8(1), 129-136.