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Antifungal assay of Solanum nigrum Linn. fruit, leaves and stems extracts in different solvents

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Research Paper 01/04/2017
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Antifungal assay of Solanum nigrum Linn. fruit, leaves and stems extracts in different solvents

Mubsher Mazher, Musfirah Anjum, Waheeda Mushtaq, Qumqum Noshad, Nafeesa Zahid Malik
Int. J. Biosci.10( 4), 380-385, April 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: Antifungal activityMinimum inhibitory concentrationSolanum nigrumZone of inhibition

Abstract

Antifungal activity of fruit, leaf and stem extract of Solanum nigrum L. prepared in four solvents i.e., ethanol, chloroform, petroleum ether and distilled water were investigated. Agar well diffusion method was utilized to study antifungal activity against five fungal strains viz. Aspergillus’s Niger, A. flavors, Saccharomyces cervisae, Alternaria alternate and Fusarium oxysprum. Zone of inhibition was measured ranging from 9.3±0.66mm of stem extracts in distilled water against A. flavus and 32.42±0.66mm of fruit extracts in chloroform against A. niger. However the stem extracts in distilled water showed no detectable zone of inhibition against A. niger and A. alternate. Minimum inhibitory concentration (MIC) of extracts of S. nigrum was measured by serial dilution method. MIC was found ranging from 250μg/ml of ethanolic leaf extracts against A. Niger to 1000μg/ml of leaf extracts in chloroform against F. oxysporum. Fruit extracts in chloroform and leaf extracts in ethanol showed greater zones of inhibition as compared to standard antifungal Grisofulvin. Fruit extracts in ethanol showed 32.42±0.66mm and fruit extracts in ethanol showed 28.16±0.72mm zone of inhibition against A. Niger and F. oxysporum respectively.

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