Antifungal assay of Solanum nigrum Linn. fruit, leaves and stems extracts in different solvents

Paper Details

Research Paper 01/04/2017
Views (337) Download (94)

Antifungal assay of Solanum nigrum Linn. fruit, leaves and stems extracts in different solvents

Mubsher Mazher, Musfirah Anjum, Waheeda Mushtaq, Qumqum Noshad, Nafeesa Zahid Malik
Int. J. Biosci.10( 4), 380-385, April 2017.
Certificate: IJB 2017 [Generate Certificate]


Antifungal activity of fruit, leaf and stem extract of Solanum nigrum L. prepared in four solvents i.e., ethanol, chloroform, petroleum ether and distilled water were investigated. Agar well diffusion method was utilized to study antifungal activity against five fungal strains viz. Aspergillus’s Niger, A. flavors, Saccharomyces cervisae, Alternaria alternate and Fusarium oxysprum. Zone of inhibition was measured ranging from 9.3±0.66mm of stem extracts in distilled water against A. flavus and 32.42±0.66mm of fruit extracts in chloroform against A. niger. However the stem extracts in distilled water showed no detectable zone of inhibition against A. niger and A. alternate. Minimum inhibitory concentration (MIC) of extracts of S. nigrum was measured by serial dilution method. MIC was found ranging from 250μg/ml of ethanolic leaf extracts against A. Niger to 1000μg/ml of leaf extracts in chloroform against F. oxysporum. Fruit extracts in chloroform and leaf extracts in ethanol showed greater zones of inhibition as compared to standard antifungal Grisofulvin. Fruit extracts in ethanol showed 32.42±0.66mm and fruit extracts in ethanol showed 28.16±0.72mm zone of inhibition against A. Niger and F. oxysporum respectively.


Abad M, Ansuategui M, Bermejo P. 2007. Active Antifungal Substances From Natural Sources. Arkivoc 7, 116-145.

Akansha S, Kumar PT, Sudeep R, Feroz K, Ashok S. 2008. Effects of Herbal Components on Human Drugs Interactions Involving Cytochrome. Bioinformation 3(5), 198-204.

Almazini MA, Hammed GA, Amani A. 2009. Antibacterial Activity of the Solasodine of Solanum nigrum L. Against Bacterial Isolated From the Wounds. Bas. J. Vet. Res 8(2), 137.

Atanu FO, Ebiloma UG, Ajayi EI. 2011. A Review of the Pharmacological Aspects of Solanum nigrum L. Biotech. Mol. Bio. Review 6(1), 1-7.

Baker JT, Borris RP, Carte B. 1995. Natural Products Drug Discovery and Development, New Perspectives on International Collaborations, Natural Products 58, 1325-1357.

Baohung JI. Report of Scientific Working Group on Leprosy. (2002). TDR/SWG/02.

Gogoi P, Muhammad I. 2012. Phytochemical Screening of Solanum nigrum L. and Solanum myriacanthus D. from Districts of Upper Assam, India. IOSR J. Pharma 2(3), 455-459.

Indhumathi T, Mohandass S. 2014. Efficacy of Ethanolic extracts of Solanum incanum fruit extracts for its antimicrobial activity. International Journal of Current Microbiology and Applied Sciences 3(6), 939-949.

Iwu MW, Duncan AR, Okunji CO. 1999. New Antimicrobials of Plant Origin. Janick J. Ed 6, 457-462. In: J. Janick (Ed.), Perspectives on new crops and new uses. ASHS Press, Alexandria, VA.

Jorgensen JH, Turnidge JD. 2007. Susceptibility Test Methods. Manual of Clinical Microbiology 9, 1152-1172.

Kiran KR, Rani M, Pal A. 2009. Reclaiming Degraded Land in India Through the Cultivation of Medicinal Plants. Bot. Res. Int 2, 174-181.

Mazher M, Nafeesa ZM, Muhammad R, Adil H, Yasir A. Qum QN. 2016. Phytochemistry and Antbaterial Assay of Fuit, Leaf and Stem Extracts of Solanum nigrum L. in Different Solvents. International Journal of Biosciences 9(6), 129-136.

Raju K, Anbuganapathi G, Gokulakrishnan V, Pajkapoor B, Manian S. 2003. Effects of Dried Fruits of Solanum nigrum L. Against Carbon Tetrachloride Induced Hepatic Injury in Rats. Bio. Pharma. Bulletin 16, 1618-1619.

Sikdar M, Dutta U. 2008. Traditional phytotherapy among the Nat people of Assam., Ethno-med 2, 39-45.

Zakaria ZA, Gopalan HK, Zainal H. 2006. Antinociceptive, Anti-inflammatory and Antipyretic Effects of Solanum nigrum Chloroform Extract in Animal Models. Yakugaku Zasshi, 126, 1171-1178.

Zubaida Y, Zabta KS, Mir AK. 2010. Phonetic Analysis of Medicinally Important Species of the Genus Solanum from Pakistan. Pakistan Journal of Botany 42(3), 1827-1833.