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Antimalarial potential of leaves crude extract of Callistemon lanceolatus D.C.

Research Paper | November 1, 2017

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Muhammad Binyamin Khan, Muhammad Afzal, Yasir Ihtesham, Muhammad Sadiq, Ejaz Ahmad Khan, Muhammad Fiaz, BurhanUd Din, Muhammad Idrees Khan, Muhammad Safdar Baloch, Najeeb Ullah

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Int. J. Biosci.11( 5), 11-20, November 2017

DOI: http://dx.doi.org/10.12692/ijb/11.5.11-20


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Plants have been used from ancient times for various purposes like food, shelter and also as a medicine to treat various diseases. The current study was based on an investigation of cheap and herbal drugs against malarial parasite. For the study, chloroform fractions of Callistemon lanceolatus of dose concentration 200µl/ml were applied against the malaria parasite. The blood samples (n=67) from male and female of various ages were collected from district Bannu. It was identified that 200µl/ml of chloroform fraction application demonstrated 54.58% inhibition (males=54.70%) of the malarial parasite. The Nivaquine (40 µg/ml) used as positive control exhibited 100% inhibition of malaria parasite. Additionally, distilled water used as negative control (200µl/ml) showed neutral effect. Conclusively, it is found that the chloroform fractions (200µl/ml) of Callistemon lanceolatus are effective against malaria disease and may the varied dose concentration be more profound against the disease.


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Antimalarial potential of leaves crude extract of Callistemon lanceolatus D.C.

Burchett M, Mousine R, Tarran J. 2002. Phytomonitoring for urban environmental management. Air Pollution Plant Biotechnology 61-91.

Cho D, Kim KH, Park SC, Kim YK, Lee KN, Lim CS. 2001. Evaluation of rapid imamunocapture assays for diagnosis of Plasmodium vivax in Korea. Parasitology Research 87, 445-448.

El Tahir A, Satti GM, Khalid SA.  1999. Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on Maytenus senegalensis (Lam.) Journal of Ethnopharmacology 64, 227-233.

Firoz M, Bharatesh K, Nilesh P, Vijay G, Tabassum S, Nilofar N. 2011. Cardioprotective activity of ethanolic extract of Callistemon lanceolatus leaves on doxorubicin-induced cardiomyopathy in rats. Bangladesh Journal of Pharmacology 6(1), 38-45. http://dx.doi.org/10.3329/bjp.v6i1.8154

Gupta A, Gupta R. 1997. A survey of plants for anticholinestrase activity. Phytochemistry 46, 827-831.

Jain AK, Dubey SK, Sikarwar MS, Jain SK. 2007. Hepatoprotective activity of methanolic extract of leaves of Callistemon lanceolatus. International Journal of Plant Sciences 2, 185-186.

Ji T. 2009. Traditional Chinese Medicine Pills for Treating Hemorrhoid. CN 101-128.

Jurg A, Tomás T, Pividal J. 1991. Antimalarial activity of some plant remedies in use in Marracuene, southern Mozambique. Journal of Ethnopharmacology 33, 79-83.

Kanjilal PC, Das A. 1992. Flora of Assam, New Delhi, Omsons Publications New Delhi.

Khalil MY, Moustafa AA, Naguib NY. 2007. Growth, phenolic compounds and antioxidant activity of some medicinal plants grown under farming  condition. World Journal of Agricultural Sciences 3, 451-457.

Kim JH, Byun JC,  Bandi AKR, Hyun CG, Lee NH.  2009. Compounds with elastase inhibition and free radical scavenging activities from Callistemon lanceolatus.  Journal of Medicinal Plant Research 3(11), 914-920.

Kim JH, Byun JC, Bandi AKR, Hyun CG, Lee NH. 2009. Compounds with elasrtase inhibition and free radical scavenging activities from Callistemon lanceolatus. Journal of Medicinal Plant Research 3,  9-14.

Kraft C, Jenett-Siems K, Siems K, Jakupovic J, Mavi S, Bienzle U, Eich E. 2003. In vitro antiplasmodial evaluation of medicinal plants from Zimbabwe. PhytotherapyResearch 17, 123-128. http://dx.doi.org/10.1002/ptr.1066

Krettli AU. 2009. Antimalarial drug discovery: screening of Brazilian medicinal plants and purified compounds. Expert Opinion Drug Discovery 4, 95-108. http://dx.doi.org/10.1517/17530050802678127

Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. 2007. Ethnopharmacological approaches to wound healing-Exploring medicinal plants of India. Journal of Ethnopharmacology 114(2), 103-113. http://dx.doi.org/10.1016/j.jep.2007.08.010

Kumar S, Kumar V, Prakash O. 2011. Antidiabetic, hypolipidemic and antioxidant activities of Callistemon lanceolatus leave extract. Journal of Herbs Spices and Medicinal Plants 17(2), 144-153. http://dx.doi.org/10.1080/10496475.2011.583139

Kumar S, Kumar V, Prakash O.  2011. Pharmacognostic study and antiinflammatory activity of Callistemon lanceolatus leaf. Asian Pacific Journal of Tropical Biomedicine 1(3), 177-181. http://dx.doi.org/10.1016/S2221-1691(11)60022-1

Lorke D. 1983. A new approach to practical acute toxicity testing. Archives of Toxicology 54, 275-287.

Lounasmaa M, Puri HS, Widen CJ. 1977. Phloroglucinol derivatives of Callistemon Lanceolatus leaves. Phytochemistry 16, 1851-1852.

Mahmoud II, Moharram FA, Marzouk MS, Linscheid MW, Saleh MI. 2002b. Polyphenolic  constituents  of  Callistemon lanceolatus leaves. Pharmacies 57, 494-6.

Marzouk MSA. 2008. An acylated flavonol glycoside and hydrolysable tannin from Callistemon lanceolatus flowers and leaves. Phytochemical Analysis 19, 541-549.

Mishra K, Dash AP, Swain BK, Dey N. 2009. Anti-malarial activities of andrographis paniculata and hedyotis corymbosa extracts and their combination with curcumin. Malaria Journal 8, 8-26.

Park SY, Lim JY, Jeong W, Hong SS, Yang YT, Hwang BY, Lee D. 2010. C-methylflavonoids isolated from Callistemon lanceolatus protect PC12 cells against Abeta-induced toxicity. Planta Medica 9, 863-868.

Peters W, Portus JH. Robinson BL. 1975. The chemotherapy of rodent malaria. The value of drug resistant strains of P. berghei in screening for blood schizontocidal activity. Annals ofTropical Medicine and Parasitology 69, 155-171.

Rao KVB, Paluri V, Ravichandran S, Kumar G, Karthik L. 2012. Phytochemical composition and in vitro antimicrobial activity of methanolic extract of Callistemon lanceolatus D.C. International Journal Pharmaceutical Sciences 4, 699-702.

Spencer RD, Lumley PF. 1991. Callistemon:  In  Flora  of  New  South Wales,Sydney, Australia, New South Wales University Press.

Sudhakar M, Rao CV, Rao AL, Raju DB. 2004. Antinociceptive and antiinflammatory effects of the standardized oil of Indian Callistemon lanceolatus leaves in experimental animals.  Acta Pharmaceutica Turcica 46, 131-139.

Trager W, Jensen JB. 1976. Human malaria parasite in continuous culture. Science 193, 673-675.

Wheeler GS. 2005. Maintenance of a Narrow Host Range By Oxypos vitiosa: A Biological  Control  Agent  of  Melaleuca. Biochemical System Ecology 33, 365-383.

Younes MEG. 1975. The extraction of C. lanceolatus leaves. Australian Journal of Chemistry 28, 2-21.