Antimicrobial activities and GC-MS chemical profiling of methanol extracts of different parts of Morus alba (Tut) in Bangladesh

Paper Details

Research Paper 01/05/2021
Views (936)
current_issue_feature_image
publication_file

Antimicrobial activities and GC-MS chemical profiling of methanol extracts of different parts of Morus alba (Tut) in Bangladesh

Md. Sabbir Hasan, Mahci Al Bashera, Shyama Prosad Moulick, Fatema Tuz Jubyda, Md. Jahidul Islam, Mst. Sarmina Yeasmin, Md. Badrul Islam
Int. J. Biosci. 18(5), 156-166, May 2021.
Copyright Statement: Copyright 2021; The Author(s).
License: CC BY-NC 4.0

Abstract

Morus alba has potential therapeutic value in different medicinal parts. We investigated the chemical differences presence in the methanolic extract (ME) of leaves, stem and root M. alba using GC-MS technique. GC-MS based phyto-chemical profiling revealed the presence of potent bioactive components in methanolic extracts of different parts (leaves, stem and root) of M. alba. Further, GC-MS study confirmed the occurrence of twenty to thirty bioactive constituents ranging from 0.06 to 32% based on their peak area. Among different methanolic extract of M. alba maximum number of phytocompounds were observed in leaves extract with glycerin (32.77%) as predominant compound and lowest in root bark (SBP) extract which is dominated hexadecanoic acid methyl ester (30.55%) respectively. Furthermore, all the study extracts were also screened for their antimicrobial activity and only stem bark extract (SBP) were found to active against both gram positive and gram negative bacteria but all are inactive against fungal strain.

Abdullah, Al-shwyeh Hussah, Mohamed Elwathig, Saeed Mirghani, Parveen Jamal. 2011. “Antibacterial Activity of Malaysian Mango Kernel” 10(81), 18739–48. https://doi.org/10.5897/AJB11.2746.

Avci, Emre. 2016. “Antioxidant and Antimicrobial Activities with GC/MS Analysis of the Morus alba L. Leaves,” no. March. http://dx.doi.org/10.17350/HJSE19030000006

Bayer AW, Kirby WMM, Sherris JC, Turck M. 1966. “Antibiotic Susceptibility Testing by a Standardized Single Disc Method.” Americal Journal of Clinical Pathology 45(4), 493–96.

Choi Youngmin, Junsoo Lee. 2009. “Antioxidant and Antiproliferative Properties of a Tocotrienol-Rich Fraction from Grape Seeds.” Food Chemistry 114(4), 1386—1390. https://doi.org/10.1016/j.foodchem.2008.11.018.

Datta RK. 2000. “Mulberry Cultivation and Utilization in India.” FAO Animal Production and Health Paper, 45–62.

Devi, Bandna, Neha Sharma, Dinesh Kumar, Kamal Jeet. 2013. “Morus Alba Linn: A Phytopharmacological Review.” International Journal of Pharmacy and Pharmaceutical Sciences 5(2), 14–18.

Hajji, Mohamed, Raoudha Jarraya, Imen Lassoued, Ons Masmoudi, Mohamed Damak, Moncef Nasri. 2010. “GC/MS and LC/MS Analysis, and Antioxidant and Antimicrobial Activities of Various Solvent Extracts from Mirabilis Jalapa Tubers.” Process Biochemistry 45(9), 1486–93. https://doi.org/10.1016/j.procbio.2010.05.027.

Joshi, Shivani, Devendra Mishra, Ganga Bisht, Khadak Singh Khetwal. 2011. “Essential Oil Composition and Antimicrobial Activity of Lobelia Pyramidalis Wall.” EXCLI Journal 10, 274–79.

Kim, Jung-Hoon, Eui-Jeong Doh, Guemsan Lee. 2020. “Quantitative Comparison of the Marker Compounds in Different Medicinal Parts of Morus Alba L. Using High-Performance Liquid Chromatography-Diode Array Detector with Chemometric Analysis.” Molecules 25(23), 5592.

Kumaradevan G, Damodaran R, Mani P, Dineshkumar G, Jayaseelan T. 2015. “Phytochemical Screening and GC-MS Analysis of Bioactive Components of Ethanol Leaves Extract Of Clerodendrum Phlomidis (L.)”. American Journal of Biological and Pharmaceutical Research 2, 142–48.

Mahboubi, Mohaddese. 2019. “Morus Alba (Mulberry), a Natural Potent Compound in Management of Obesity.” Pharmacological Research 146, 104341. https://doi.org/10.1016/j.phrs.2019.104341.

Perelman, Patricia, Ana Faggi, Maria Castro, Eduardo Martinez Carretero. 2010. “Pollution Trends Using Bark of Morus Alba in the Cities of Buenos Aires and Mendoza (Argentina).” Revista Árvore 34(3), 505–11. https://doi.org/10.1590/s010067622010000300014.

Pinto Maria EA, Sthéfane Araújo G, Marcela Morais I, Nívea Sá P, Caroline Lima M, Carlos Rosa A, Ezequias Siqueira P, Susana Johann, Luciana Lima ARS. 2017. “Antifungal and Antioxidant Activity of Fatty Acid Methyl Esters from Vegetable Oils.” Anais Da Academia Brasileira de Ciencias 89(3), 1671–81. https://doi.org/10.1590/0001-3765201720160908.

Rahman MM, Ahmad SH, Mohamed MTM, Ab Rahman MZ. 2014. “Antimicrobial Compounds from Leaf Extracts of Jatropha Curcas, Psidium Guajava, and Andrographis Paniculata.” Edited by Prasat Kittakoop. The Scientific World Journal 635240. https://doi.org/10.1155/2014/635240.

Rahman Md. Munsur. 2014. “Investigation Some Physicochemical Properties, Lipids, Glycerides and Fatty Acid Composition of Mulberry (Morus Alba L) Seed Oil of Three Different Regions of Bangladesh.” American Journal of Applied Chemistry 2(3), 38. https://doi.org/10.11648/j.ajac.20140203.11.

Salem, Mohamed Zidan Mohamed, Hussein Aly, Yousry Gohar, Abdel-Wahab El-Sayed. 2013. “Biological Activity of Extracts from Morus Alba L., Albizzia Lebbeck (L.) Benth. and Casuarina Glauca Sieber Against the Growth of Some Pathogenic Bacteria.” International Journal of Agricultural and Food Research 2(1), 9–22. https://doi.org/10.24102/ijafr.v2i1.122.

Saravanan P, Chandramohan G, Mariajancyrani J, Shanmugasundaram P. 2014. “GC-MS Analysis of Phytochemical Constituents in Ethanolic Bark Extract of Ficus Religiosa Linn.” International Journal of Pharmacy and Pharmaceutical Sciences 6 (January), 457–60.

Vijayan, Kunjupillai, A Tikader, Zhao Weiguo, Chirakkara Nair, Chi-Hua Tsou. 2011. “Morus,” June. https://doi.org/10.1007/978-3-642-20447-0_5.

Wang, Wei, Yuangang Zu, Yujie FU, Thomas Efferth. 2012. “In Vitro Antioxidant and Antimicrobial Activity of Extracts from Morus Alba L. Leaves, Stems and Fruits.” American Journal of Chinese Medicine 40(2), 349–56. https://doi.org/10.1142/S0192415X12500279.

Zhou BL, Chen ZX, Du L, Xie YH, Zhang Q, Ye XL. 2011. “Allelopathy of Root Exudates from Different Resistant Eggplants to Verticillium Dahliae and the Identification of Allelochemicals” 10(42), 8284–90. https://doi.org/10.5897/AJB10.2300.

Related Articles

The role of aberrant glycosylation in autoimmune disease development and progression

Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam*, Int. J. Biosci. 28(6), 1-12, June 2026.

Seasonal variations in bed bug (Cimex spp.) populations in several public places in Korhogo (Ivory Coast): Ecological approaches and epidemiological perspectives

Toure Donatie Serge*, Traore Issouf, Traore Mamadou, Ouattara Manzara, Ouattara Foungoye Allassane, Kone Mouhamadou, Doumbia Mamadou, Int. J. Biosci. 28(5), 76-83, May 2026.

Molecular surveillance of African swine fever virus in raw pork and blood samples from wet markets and abattoirs in Tuguegarao City, Cagayan

Hannah Lee R. Guirren*, Benjamin Abella, Aira D. Cuarteros, Int. J. Biosci. 28(5), 66-75, May 2026.

Limonene-enhanced botanical fungicides: A sustainable component of integrated tomato powdery mildew management in the tropical region

Edmund F. Luena*, Angela G. Mkindi, Akida I. Meya, Nelson S. Mpumi, Steven R. Belmain, Int. J. Biosci. 28(5), 52-65, May 2026.

Kapwa (Shared identity): The case of being community health workers (CHWs) in Cagayan Province, Philippines

Jay Emmanuel L. Asuncion, Julius T. Capili, Jinky Marie T. Chua*, Pauline Grace P. Casil-Batang, Lara Melissa G. Luis, Dorina D. Sabatin, Krisha Anne A. Hipolito, Ethel Marie M. Mangada, Int. J. Biosci. 28(5), 43-51, May 2026.

Effects of corn silage-based diets on carcass and meat characteristics of Philippine native swamp buffalo (Bubalus bubalis carabanensis)

Vince Randolf R. Sumajit*, Phoebe Lyndia T. Llantada, Ann-Sherly R. Dugyon, Int. J. Biosci. 28(5), 36-42, May 2026.

Assessment of the knowledge, attitude, and practices of frontliners toward community-acquired pneumonia in the Cagayan, Philippines

Jinky Marie T. Chua*, Nikko Alexander S. Pacquing, Ann P. Chua, Ethel Marie M. Mangada, Int. J. Biosci. 28(5), 26-35, May 2026.