Antimicrobial analysis of root and shoot extracts of Ephedra intermedia Schrenk & Meyer

Paper Details

Research Paper 01/09/2017
Views (354) Download (9)
current_issue_feature_image
publication_file

Antimicrobial analysis of root and shoot extracts of Ephedra intermedia Schrenk & Meyer

Muhammad Sabir Bangulzai, Saadullah Khan Leghari, Imran Ali, Mudassir Asrar, Tariq Ismail, Ayesha Mushtaq
Int. J. Biosci.11( 3), 86-94, September 2017.
Certificate: IJB 2017 [Generate Certificate]

Abstract

This study was done to estimate the antimicrobial activity of root and shoot extracts of Ephedra intermedia Schrenk & Meyer. The root and shoot extracts of Ephedra intermedia was tested against four Bacterial strains (Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens and Bacillus subtilis) and three fungal species (Fusarium verticilliodes, Aspergillus lavus and Dreschlera turcicia). In this study the Ephedra intermedia (root and shoot) methanol extract showed highest antibacterial actions against Staphylococcus aureus, followed by Escherichia coli and lowest activity was noted against Bacillus subtilis. The alcoholic and distilled water extracts of E. intermedia root and shoot also showed significant activity against all examined fungal species. The extreme antifungal activity through methanol extraction shown against Fusarium verticilliodes, which followed by Aspergillus flavus and lowest movement was reported against Dreschlera turcicia with minimum inhibition zone. Further that the methanol extract exhibited significant effect against all the investigated bacterial and fungal species as compared to the ethanol and distilled water extract. This study also showed that the roots of E. intermedia were more effective as compared to the shoots against under study bacterial and fungal species.

VIEWS 10

Ajaib M, Ali S, Khan Z. 2014. “Antioxidant and antimicrobial activities of an ethnobotanically important plant Notholirion thomsonianum from district Kotli, Azad Jammu & Kashmir,” J. Ani and P. Sci 24(3). 774-780.

Ajaib M, Hussain T, Farooq S, Ashiq M. 2016. Analysis of Antimicrobial and Antioxidant Activities of Chenopodium ambrosioides: An Ethnomedicinal Plant, Hindawi Publishing Corporation. J. Chemistry 1-11. http://dx.doi.org/10.1155/2016/4827157.

Ajaib M, Khan KM, Perveen S, Shah S. 2013b. Antimicrobial and antioxidant activities of Echinochloa colona (Linn.) Link and Sporobolus coromandelianus (Retz.) Kunth. J. Chem. Soc. Pak 35(3), 960-965.

Ajaib M, Zikrea A, Khan KM, Perveen S, Shah S, Karim A. 2013a. Rivina humilis L: A potential antimicrobial and antioxidant source. J. Chem. Soc. Pak 35(5), 1384-1398.

Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. 1985. Natural plant chemicals: Sources of Industrial and Medicinal materials. Science 228, 1154-1160.

Cheruiyot K, Olila R, Kateregga D. 2009. In vitro antibacterial activity of selected medicinal plants from Longisa region of Bornet district, Kenya. Afr. J. Health Sci 9(1), 42-46.

Doss AR. 2008. Preliminary phytochemical screening and antibacterial studies of leaf extracts of Solanum trilobatum Linn. Ethnobotanical leaflets 6(12), 42.

Jameela M, Mohideen A, Sunitha K, Narayanan M. 2011. Antibacterial Activities of Three Medicinal Plants Extract against Fish Pathogens. Inte. J. Bio. Tech 2(2), 57-60.

Kirbag S, Zengin F, Kursat M. 2009. Antimicrobial Activities of Extracts of some Plants. Pak. J. Bot 41(4), 2067-2070.

Kumara M, Agarwala R, Deyb K, Raib V, Johnsonc B. 2009. Antimicrobial Activity of Aqueous Extract of Terminalia chebula Retz. on Gram positive and Gram negative Microorganisms. Inte. J. Cur. Pharma.l Res 1(1), 56-60.

Mahesh B, Satish S. 2008. Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens. World. J. Agric. Sci 4(S), 839-843, 2008.

Mazhar F, Khanum R, Ajaib M, Jahangir M. 2015. “Potent AChE enzyme inhibition activity of Zizyphus oxyphylla: a new source of antioxidant compounds,” Pak. J. Phar. Sci 28(6), 2053-2059.

Mohamed A, El-Sayed M, Hegazy M, Helaly S, Esmail1 A, Mohamed N. 2010. Chemical Constituents and Biological Activities of Artemisia herba-alba. Rec. Nat. Prod 1-25.

Obeidat M, Shatnawi M, Al-alawi M, Al-Zu`bi E, Al-Dmoor H, AlQudah M, El-Qudah J, Otri I. 2012. Antimicrobial Activity of Crude Extracts of Some Plant Leaves. Res. J. Micrbi 7, 59-67.

Parekh J, Chanda S. 2006. In-vitro Antimicrobial Activities of Extracts of Launaea procumbens Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). Afri. J. Biomedical. Res 9, 89 -93.

Rahman AS, Abd-Ellatif S, Deraz S, Khalil A. 2011. Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. Afri. J. Biot 10(52), 10733-10743.

Ramzi A, Mothana A, Lindequist U. 2005. Antimicrobial activity of some plants of the Island soqotra, J. Ethnopharmacol 96(1-2). 177-181.

Seddik K, Nadjet I, Abderrahmane B, Daoud H, Lekhmici A. 2010. Antioxidant and antibacterial activities of extracts from Artemisia herba alba Asso. Leaves and some phenolic compounds. J. Medi. P. Res 4(13), 1273-280.

Shihabudeen M, Priscilla H, Thirumurugan D. 2010. Antimicrobial Activity and Phytochemical Analysis of Selected Indian Folk Medicinal Plants. Inte. J. Pharma. Sci and Res (IJPSR) 1(10), 430-434.

Shinwari ZK, Khan I, Naz S, Hussain A. 2009. Assessment of antibacterial activity of three plants used in Pakistan to cure respiratory diseases. Afri. J. Bio 8(24), 7082-7086.

Tareen RB, Bibi T, Khan MA, Ahmed M, Zafar M. 2010. Indigenous knowledge of folk medicine by the women of Kalat and Khuzdar regions of Balochistan Pakistan. Pak. J. Bot 42(3), 1465-1485.

Walter C, Shinwari ZK, Afzal I, Malik RN. 2011. Antibacterial Activity in Herbal Products Used In Pakistan. Pak. J. Bot 43(SI): 155-162.

Zaidi MA, Crow SA. 2012. Cytotxicity of Four Medicinal Plants of Pakistan. Pak. J. Bot 44(SI), 395-397.