Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Short Communications | March 1, 2013

| Download 3

Antimicrobial and cytotoxic activities of the crude extracts of Callistemon linearis

Ayesha Haque, M. Mahboob Ali Siddiqi, A. F. M. Mustafizur Rahman, A. M. Sarwaruddin Chowdhury, Choudhury M. Hasan

Key Words:

Int. J. Biosci.3( 3), 129-133, March 2013

DOI: http://dx.doi.org/10.12692/ijb/3.3.129-133


IJB 2013 [Generate Certificate]


The petroleum ether (PE), carbon tetrachloride (CT) and ethyl acetate (EA) soluble fractions of a crude methanol extract of Callistemon linearis and crude methanol (ME) extract itself were subjected to antibacterial and antifungal activities and cytotoxicity against brine shrimp nauplii. Present investigation revealed that the PE, CT and ME extracts have the potent to moderate sensitivity against almost all the bacteria and fungi, whereas crude EA extract showed only mild antimicrobial activity. The brine shrimp lethality with LC50 values were 8.16, 14.21, 0.42, 54.07 and 0.33µg/mL for PE, CT, ME, EA extracts and Vincristine sulphate (VS), respectively. Compare to Vincristine sulphate (standard), the cytotoxicity exhibited by the crude methanol (ME) extract was promising and this clearly indicates the presence of potent bioactive compounds in Callistemon linearis.


Copyright © 2013
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Antimicrobial and cytotoxic activities of the crude extracts of Callistemon linearis

Osuala FOU, Nuoli MC, Anyadoh SO. 2005. Antibacterial activity of Medicinal plants. International Journal of Natural Products Science 2(1), 36- 39.

Kanjilal PC, Das A. 1992. Flora of Assam. Omsons Publications, New Delhi, India.

Chowdhury S, Ghosh AK, Maji S, Bala NN. 2012. Phytochemical screening and isolation of a pure phytoconstituent from the leaves of Callistemon salignus. American Journal of PharmTech Research 2(3), 993- 1000.

Das A, Zaman K, Singh AV. 2009. Phytochemical and chemical composition evaluation of volatile oil of Callistemon linearis DC leaf. Advances in Natural and Applied Sciences 3(1), 56- 59.

Das A, Zaman K, Singh AV. 2008. Antimicrobial and antioxidant activities of Callistemon linearis DC leaf extract. Pharmacology 3, 875- 881.

Haque A, Siddiqi MMA, Rahman AFMM, Chowdhury AMS, Choudhury MH. 2013. Isolation of betulinic acid and 2,3-dihydroxy Olean-12-en-28-oic acid from the leaves of Callistemon linearis. Dhaka University Journal of Science, in press.

Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology. 45, 493- 496.

Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active constituents. Planta Medica 45, 31- 32.

Seyydnejad SM, Niknejad M, Darabpoor I, Motamedi H. 2010. Antibacterial Activity of Hydroalcoholic Extract of Callistemon citrinus and Albizia lebbeck. American Journal of Applied Sciences 7(1), 13- 16.

Hedrick UP. 1972. Sturtevant’s Edible Plants of the World. Dover Publications, New York, USA.