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Antimicrobial and Immunomodulatory Properties of Crude Extract and Compounds from Boswellia dalzielii Hutch

Research Paper | February 1, 2019

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Talom Tangue Benjamin, Tagne Simo Richard, Talla Emmanuel, Fodouop Chegaing Siméon Pierre, Njateng Guy Sedar Senghor, Nyemb Jean Noël, Almas Jabeen, Huma Aslam Bhatti, Rukesh Maharjan, Tatsadjieu NgouneLéopold, Kuiate Jules-Roger

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Int. J. Biosci.14( 2), 161-171, February 2019

DOI: http://dx.doi.org/10.12692/ijb/14.2.161-171


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In the North of Cameroon, Boswellia dalzielii is a medicinal plant used in traditional medicine to treat infectious diseases (dysentery, typhoid, cholera and malaria). The aim of this study is to identify and characterized the bioactive compounds from the roots of this plant. The methanol crude extract from the root of Boswellia dalzielii Hutch was obtained by maceration in methanol. Partition of part of this crude extract using solvent-solvent extraction in n-hexane, ethyl acetate, n-butanol yield hexane, yield hexane, ethyl acetate, n-butanol and residual fractions. Ethyl acetate fraction showed the highest antibacterial activity and was subjected to column chromatography. Crude extract, fractions and pure compounds were tested for their antibacterial properties using microdilution method with Alamar Blue as dye. Immunomodulatory activities were determined using chemiluminescence immunoassay. Ethyl acetate fraction exhibited highest antimicrobial activities and its fractionation afforded 3 known compounds and a mixture: trans-desoxyrhapontigenin-3-O-rutinoside (1), α-amyrin (3) and trans-desoxyrhaponticin (4) and a mixture of β-amyrin and lupeol (2) in a ratio (1:2). Mixture (2) as far as compounds (1) and (3) showed no antimicrobial activity against all bacteria strains tested while desoxyrhapontigenin-3-O-rutincompound (4) (MIC=125µg/mL) was relatively active. Ethyl acetate fraction exhibited lowest immunomodulator activity while compound (3) showed highest immunomodulating activities towards polymorphoneutrophils (IC50 < 7 µg/mL). These results showed that Boswellia dalzielii possess both antibacterial and immunomodulatory effects due to different comounds and may justify it use in traditional medicine to fight infectious diseases.


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Antimicrobial and Immunomodulatory Properties of Crude Extract and Compounds from Boswellia dalzielii Hutch

Abreu AC, McBain AJ, Simões M. 2012. Plants as sources of new antimicrobials and resistance-modifying agents. Natural Product  Reports 29(9), 1007–1021. https://doi.org/10.1039/c2np20035j

Ahmad B, Naz S, Azam S, Khan I, Bashir S, Hassan F. 2015. Antimicrobial, Phytotoxic, Heamagglutination, Insecticidal and Antioxidant Activities of the Fruits of Sarcococca Saligna (D . DON) MUEL . Pakistan Journal of Botany 47, 313–319.

Ahmed MM,  Jabeen A. 2012. In Silico and In Vitro Immunomodulatory Studies on Compounds of Lindelofia stylosa stylosa. Chem Biol Drug Des 79, 290–299. https://doi.org/10.1111/j.1747-0285.2011.01310.x

Alemika Taiwo E, Onawunmi GO, Olugbade T. A. 2004. Isolation and characterization of incensole from Boswellia dalzielii stem bark. Journal of Pharmacy and Bioresources 1(1), 7–11. https://doi.org/10.4314/jpb.v1i1.32041

Collins LA, Franzblau SG. 1997. Microplate Alamar Blue Assay versus BACTEC 460 System for High-Throughput Screening of Compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrobial Agents and Chemotherapy, 41(5), 1004–1009.

Csuk R, Barthel-Niesen A, Ströhl D, Kluge R, Wagner C, Al-Harrasi A. 2015. Oxidative and reductive transformations of 11-keto-β-boswellic acid. Tetrahedron 71(13), 2025–2034. https://doi.org/10.1016/j.tet.2015.01.062

Jansen O, Angenot L, Tits M, Nicolas JP, De Mol P, Nikiéma JB, Frédérich M. 2010. Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. Journal of Ethnopharmacology 130(1), 143–150. https://doi.org/10.1016/j.jep.2010.04.032

Jimenez-Arellanes A, Meckes M, Ramirez R, Torres J, Luna-Herrera J, Herrera JL. 2003. Activity against Multidrug-resistant Mycobacterium tuberculosis in Mexican Plants Used to Treat Respiratory Diseases. Phytotherapy Research 17, 903–908. https://doi.org/10.1002/ptr.1377

Joubouhi C, Tamokou J, Ngnokam D, Voutquenne-nazabadioko L. 2017. Iridoids from Canthium subcordatum iso -butanol fraction with potent biological activities, 1–8. https://doi.org/10.1186/s12906-016-1536-8

Kakkar S, Bais S. 2014. A Review on Protocatechuic Acid and Its Pharmacological Potential, 2014.

Kémeuzé VA, Mapongmetsem PM, Tientcheu, MA, Nkongmeneck BA, Jiofack RB. 2012. Boswellia dalzielii Hutch : État du peuplement et utilisation traditionnelle dans la région de Mbé (Adamaoua-Cameroun). Secheresse 23, 278–283. https://doi.org/10.1684/sec.2012.0365

Kuete V. 2010. Potential of Cameroonian plants and derived-products against microbial infections. Planta Medica 76, 1479–1491. https://doi.org/10.1055/s-0030-1250027

Mahomoodally F, Mesaik A, Choudhary MI, Subratty AH. 2012. In vitro modulation of oxidative burst via release of reactive oxygen species from immune cells by extracts of selected tropical medicinal herbs and food plants. Asian Pacific Journal of Tropical Medicine 5(6), 440–447. https://doi.org/10.1016/S1995-7645(12)60075-3

Ntie-Kang F, Lifongo LL, Mbaze LMA, Ekwelle N, Owono Owono LC, Megnassan E, Efange S. MN. 2013. Cameroonian medicinal plants: A bioactivity versus ethnobotanical survey and chemotaxonomic classification. BMC Complementary and Alternative Medicine 13(1), 1–18. https://doi.org/10.1186/1472-6882-13-147

O’Brien TF, Stelling J. 2011. Integrated multilevel surveillance of the world’s infecting microbes and their resistance to antimicrobial agents. Clinical Microbiology Reviews 24(2), 281–295. https://doi.org/10.1128/CMR.00021-10

Olukemi MA, Kandakai-olukemi YT. 2005. Antibacterial activity of the stem bark of Boswellia dalzielii. Journal of Pharmacy and Bioresources, 2(2), 131–136.

Oumar M, Tume C, Kamtchueng MO, Kamanyi A. 2014. In vitro effect of aqueous extract, hexane and methanol fractions of Boswellia dalzeilii, hutch (family: burseraceae) in immunomodulatory activities of human monocytes / macrophages. International Journal of Biological & Pharmaceutical Research, 5(2), 201–209.

Popova M, Dimitrova R, Al-lawati HT, Tsvetkova I, Najdenski H, Bankova V. 2013. Omani propolis : chemical profiling , antibacterial activity and new propolis plant sources. Chemistry Central Journal 7(1), 1–8. https://doi.org/10.1186/1752-153X-7-158

Sama Fonkeng L, Mouokeu RS, Tume C, Sedar G, Njateng S. 2015. Anti-Staphylococcus aureus activity of methanol extracts of 12 plants used in Cameroonian folk medicine. BMC Research Notes, 8(DECEMBER), 1–6. https://doi.org/10.1186/s13104-015-1663-1

Santé OM. de la. 2012. Statistique mondiale sanitaire.

Sharma KR, Adhikari A, Jabeen A, Dastagir N, Kalauni SK, Choudhary MI, Pokharel YR. 2015. Immunomodulatory Studies on Triterpenoids from Scoparia dulcis Linn. Biochemistry & Pharmacology, 4(4).

Usha PTA, Jose S, Nisha AR. 2010. Antimicrobial Drug Resistance – A global concern. Veterinary World 3(3), 138–139.