Antimicrobial potential of two medicinal plants (Psammogeton biternetum and Haloxylon griffithii) of District Kalat Balochistan, Pakistan

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Research Paper 01/07/2019
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Antimicrobial potential of two medicinal plants (Psammogeton biternetum and Haloxylon griffithii) of District Kalat Balochistan, Pakistan

Muhammad Bilal, Saeed-Ur-Rehman, Saadullah Khan Leghari, Kanval Shaukat, Abdul Kabir Khan Achakzai, Muhammad Anwar Khan Panezai, Aziz-Ud-Din, Sana Sadaf
Int. J. Biosci. 15(1), 120-128, July 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

The main objective of this study was to explore the medicinal potential of two important plant species including; Haloxylon griffithii and Psammogeton biternetum.  The Leaf, stem, root and seed (methanolic and ethanolic) extracts of these plant were examined against some pathogenic microbes (fungal and bacterial strains) through agar well diffusion techniques. For anti-bacterial screening 4 gram positive (Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis) and 14 gram negative (Acinetobacter baumannii, Escherichia coli, Salmonellla typhi, Proteus sp, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella paratyphi A, Salmonella enterica, Shigella boydii, Shigella sonnei, Klebsiella oxytoca, Klebsiella pneumoniae and Serratia marcescens) bacterial species were selected. For antifungal activities 5 fungal species (Candida albicans, Aspergillus niger, Penicillin, Aspergillus flavus and Mucor) were chosen for examination. Results revealed that Haloxylon griffithii leaves  was effective against all test species except Bacillus subtilis. Psammogeton biternetum was not active against Bacillus subtilis, Acinetobacter baumannii, Proteus mirabilis, Proteus vulgaris, Salmonella paratyphi A, Salmonella enterica, Shigella boydii, and Shigella sonnei. Psammogeton biternetum exhibited highest antimicrobial activity for Pseudomonas aeruginosa with 37mm ZI (zone of inhibition). Haloxylon griffithii was highly effective against Proteus with 31mm ZI. Furthermore, ethanolic extracts were found to be more effective than methanolic extracts. The extracts were least active against fungal strains. Results revealed that Candida albicans, Aspergillus flavus and Mucor are completely resistant against the selected medicinal plants.

Ajaib M, Ali S, Khan Z. 2014. Antioxidant and antimicrobial activities of an ethnobotanically important plant Notholirion thomsonianum from district Kotli, Azad Jammu & Kashmir. Journal of Animal and Plant Sciences 24, 774-780.

Ajaib M, Khan KM, Perveen S, Shah S. 2013. Antimicrobial and antioxidant activities of Echinochloa colona (Linn.) Link and Sporobolus coromandelianus (Retz.) Kunth. Journal of the Chemical Society of Pakistan 35, 960-965.

Ajaib M, Zikrea A, Khan KM, Perveen S, Shah S, Karim A. 2013. Rivina humilis L: A potential antimicrobial and antioxidant source. Journal of the Chemical Society of Pakistan 35, 1384-1398.

Ali SI, Qaiser M, Freitag H, Hedge IC, Jafri SMH, Kothe-Heinrich G, Omer S, Uotila P. 2001. Flora of Pakistan, Published by Department of Botany, University of Karachi, Karachi, Pakistan 204, 188-190.

Ali SI, Qaiser M. 2001. Flora of Pakistan Chenopodiaceae Jointly published by Department of Botany, University of Karachi, Karachi, Pakistan & Missouri Botanical Press: Missouri Botanical Garden, St Louis, Mussouri USA 204, 185.

Aziz-UL-Ikram NBZ, Shinwari ZK, Mohammad Q. 2015. Ethnomedicinal review of folklore medicinal plants belonging to family Apiaceae of Pakistan. Pakistan Journal of Botany 47, 1007-1014.

Bhalodia NR, Shukla VJ. 2011. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l: An ethnomedicinal plant. Journal of Advanced Pharmaceutical Technology & Research 2, 104-109.

Bibii T, Ahmad A, Baloch AI, Muhammad S, Manzoor R. 2017. Ethnomedicinal uses of plants for child birth and postpartum recovery in district Pishin, Northern Balochistan, and Pakistan. International Journal Biological Pharmacy and Allied Sciences 6, 1730-1760.

Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D. 2009. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical infectious diseases 48, 1-12.

Chaman S, Sharma G, Shalini, Reshi AK. 2013. Study of Antimicrobial properties of Catharanthus roseus by Agar Well Diffusion method. International Research Journal of Pharmaceutical and Applied Sciences 5, 65-68.

Cheruiyot K, Olila R, Kateregga D. 2009. In vitro antibacterial activity of selected medicinal plants from Longisa region of Bornet district, Kenya. African Health Sciences 9, 42-46.

Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totte J, Pieters L, Vlietinck AJ, 2002. Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of ethnopharmacology 79, 213-220.

Deepak S, Pawar A, Shinde P. 2014. Study of antioxidant and antimicrobial activities of Andrographis paniculate, Asian Journal of Plant Science and Research 4, 31-41.

Doughari JH. 2006. Antimicrobial Activity of Tamarindus indica Linn. Tropical Journal of Pharmaceutical Research 5, 597-603.

Farnsworth NR, Soejarto DD. 1991. Global importance of medicinal plants. In: Akerele, O., Heywood, V., Synge, H. (Eds.). The Conservation of Medicinal Plants. Cambridge University Press, Cambridge, 25–52.

Giamarellou H. 2010. Multidrug-resistant Gram-negative bacteria: how to treat and for how long. International Journal of Antimicrobial Agents 36, 50-54.

Hediat MH, Salama, Najat Marraiki. 2010. Antimicrobial activity and phytochemical analyses of Polygonum aviculare L. (Polygonaceae), naturally growing in Egypt. Saudi Journal of Biological Sciences 17, 57–63.

Kumar AO, Mutyala NL, Raja Rao KG. 2010. Antibacterial Evaluation of Snake Weed (Euphorbia Hirta L.), International Journal of Pharm Tech Research 2, 1383-1385.

Kunwar RM, Bussmann RW. 2008. Ethnobotany in the Nepal Himalaya. Journal of Ethnobiology and Ethnomedicine 4, 24.

Marasini BP, Baral P, Aryal P, Ghimire KR, Neupane S. 2015. Evaluation of antibacterial activity of some traditionally used medicinal plants against human pathogenic bacteria. BioMed Research International 2015, 6 pages, Article ID 265425, http://dx.doi.org/10.1155/2015/265.425

Martins AP, Salgueiro L, Goncalves MJ, Proencacunha V, Vila R, Canigueral S, Mazzoni V. 2001. Essential oil composition and antimicrobial activity of three Zingiberaceae from S.Tomeeprincipe, Journal of Planta Medica 67, 580-584.

Mhaskar KS, Blatter E, Caius JF. 2000. Indian Medicinal plants published by Indian Books centre, Delhi, India IV, 1212-1214.

Rahimi-Nasrabadi M, Gholivand MB, Batooli H, Vatanara A. 2009. Chemical Composition of the Essential Oil from Leaves and Flowering Aerial Parts of Psammogeton canescens (DC.) Vake from Iran. Journal of Medicinal Plants 8, 82-86.

Rahman SA, Abd-Ellatif SA, Deraz SF, Khalil AA, 2011. Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. African Journal of Biotechnology 10, 10733-10743.

Ramya S, Govindaraji V, Kannan KN, Jayakumararaj R. 2008. In Vitro-Evaluation of Antibacterial Activity Using Crude Extracts of Catharanthus roseus L. (G.) Don, Ethno botanical Leaflets 1, 1067-72.

Ramzi A, Mothana A, Lindequist U. 2005. Antimicrobial activity of some plants of the Island soqotra. Journal of Ethnopharmacology 96, 177-181.

Samy RP, Ignacimuthu S. 2000. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats in India. Journal of Ethnopharmacology 69, 63-71.

Suneetha G, Ravi V. 2013. Antimicrobial Activity of Andrographis paniculata Flower Extracts, International Journal of Research and Reviews in Pharmacy and Applied science 2, 604-610.

Tareen RB, Bibi T, Khan MA, Ahmad M, Zafar M. 2010. Indigenous knowledge of Folk medicine by the women of Kalat and Khuzdar regions of Balochistan, Pakistan. Pakistan Journal of Botany 42, 1465-1485.

Thongson C, Davidson PM, Mahakarnchanakul W, Weiss J. 2004. Antimicrobial activity of ultrasound assisted solvent-extracted spices. Letters in Applied Microbiology 39, 401-6.

Tona L, Kambu K, Ngimbi N, Cimanga K, Vlietinck AJ. 1998. Antiamoebic and phytochemical screening of some Congolese medicinal plants. Journal of Ethnopharmacol 61, 57-65.

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