home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Antioxidant, analgesic, anti-inflammatory and CNS depressant activities of ethanolic and combined extracts of Pandanus foetidus and Alangium salvifolium: In vitro and In vivo studies

Paper Details

Research Paper 03/09/2023
Views (668) Download (102)
current_issue_feature_image
publication_file

Antioxidant, analgesic, anti-inflammatory and CNS depressant activities of ethanolic and combined extracts of Pandanus foetidus and Alangium salvifolium: In vitro and In vivo studies

Nurunnahar, Badrul Islam, Shikha Khatun, Akteruzzaman Chowdhury, Ashik Mosaddik, Imtiaj Hasan
Int. J. Biosci.23( 3), 52-68, September 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: AnalgesicAnti-inflammatoryCNS- depressantCombined extract

Abstract

The present study investigated the phytochemical, antioxidant and in vivo biological activities such as anti-inflammatory, analgesic and CNS depressant activities of the ethanolic extracts of Pandanus foetidus (leaves), Alangium salvifolium (leaves and flowers) and combined extract of these plant species. In the DPPH radical scavenging activity assay, IC50 value of 28.5 µg/ml was found for the combined extract whereas the total antioxidant capacity (0.94±0.3) of that extract was concentration-dependent. All the extracts contained polyphenols and flavonoids but higher values of total phenolic (33.33 ± 5.06 mg/g) and total flavonoid (318.4 ± 4.39 mg/g) content were determined for the combined extract. The combined extract showed higher writhing inhibition (81%; 100mg/Kg body weight) among all the extracts which is quite comparable to the standard drug diclofenac sodium (87.17%; 10mg/Kg body weight). Anti-inflammatory activity of all the extracts was found to be moderate, but the combined extract showed higher activity (87.5% at 100 mg/kg body weight) in mice when compared to the standard (91.25% at 10 mg/kg body weight). The CNS depressant activity of combined extract was 39.75±2.63 at 90 min after administration of the extract, which was 28.25±1.46 for the standard. Our findings suggested that the combined extract of these species can be used as an herbal remedy in pain, inflammatory and CNS disorders. This is the first time that phytochemical and pharmacological activities of combination of extracts of these plant species have been reported.

VIEWS 184
Cover PDF

Abah SE, Egwari LO. 2011. Methods of extraction and antimicrobial susceptibility testing of plant extracts. African Journal of Basic and Applied Sciences 3(5), 205-209.

Ambade A, Mandrekar P. 2012. Oxidative stress and inflammation: essential partners in alcoholic liver disease. International journal of hepatology, 853175.

Anjum A, Haque ME, Rahman MM, Sarker SD. 2002. Antibacterial compounds from the flowers of Alangium salviifolium. Fitoterapia 73(6), 526-528.

Aovi FI, Rahman MM. 2019. Determination of different antioxidant compounds from the mangrove plants of Pandanus foetidus and Avicennia officinalis. Pharmacology Online 1, 279-288.

Arct J, Pytkowska K. 2008. Flavonoids as components of biologically active cosmeceuticals. Clinics in dermatology 26(4), 347-357.

Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S, Norhaizan ME, Kumar SS. 2016. Role of antioxidants and natural products in inflammation. Oxidative medicine and cellular longevity, 5276130.

Bhosale AS, Singh G. 2020. Antioxidant potential of different solvent extracts isolated from Alangium salvifolium flowers. European Journal of Molecular and Clinical Medicine 7(9), 2020.

Biswas S, Das R, Banerjee ER. 2017. Role of free radicals in human inflammatory diseases. Aims Biophysics 4(4), 596-614.

Briskin DP. 2000. Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant physiology, 124(2), 507-514.

Choi HY, Jhun EJ, Lim BO, Chung IM, Kyung SH, Park DK. 2000. Application of flow injection—chemiluminescence to the study of radical scavenging activity in plants. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 14(4), 250-253.

Cragg GM, Newman DJ, Sander KM. 1997. Natural products in drug discovery and development. Journal of Natural Products 60, 52–60.

Dal Molin MM, Silva S, Alves DR, Quintao NLM, Delle Monache F, Filho VC, Niero R. 2012. Phytochemical analysis and antinociceptive properties of the seeds of Garcinia achachairu. Archives of pharmacal research 35, 623-631.

De B, Singla RK, Bhandari K, Katakam P, Adiki SK, Mitra A. 2017. Study the enzyme inhibitory potentialities of a phytocomposite for type 2 diabetes by in silico GRIP docking. International Journal of Pharmaceutical and Life Sciences 5, 34-57.

Dehshahri SH, Wink M, Afsharypuor S, Asghari G, Mohagheghzadeh A. 2012. Antioxidant activity of methanolic leaf extract of Moringa peregrina (Forssk.) Fiori. Research in Pharmaceutical Sciences 7(2), 111-118.

Demsie DG, Yimer EM, Berhe AH, Altaye BM, Berhe DF. 2019. Anti-nociceptive and anti-inflammatory activities of crude root extract and solvent fractions of Cucumis ficifolius in mice model. Journal of Pain Research 12, 1399-1409.

Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry 50(10), 3010-3014.

Dey P, Chandra S, Chatterjee P, Bhattacharya S. 2011. Neuropharmacological properties of Mikania scandens (L.) willd (asteraceae). Journal of advanced pharmaceutical technology and research 2(4), 255.

Duthie GG, Duthie SJ, Kyle JA. 2000. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutrition research reviews 13(1), 79-106.

Elisabetsky E, Amador TA, Albuquerque RR, Nunes DS, do CT Carvalho A. 1995. Analgesic activity of Psychotriacolorata (Willd. ex R. & S.) Muell. Arg. alkaloids. Journal of Ethnopharmacology 48(2), 77-83.

Farooq S, Ngaini Z. 2021. Natural and synthetic drugs as potential treatment for coronavirus disease 2019 (COVID-2019). Chemistry Africa 4(1), 1-13.

Garcia-Perez P, Lozano-Milo E, P. Gallego-Veigas PP, Tojo SMC, Losada-Barreiro S, Bravo-Diaz CD. 2019. Some New Aspects of Colloidal Systems in Foods, In: Plant Antioxidants in Food Emulsions. IntechOpen.

Geremew H, Shibeshi W, Tamiru W, Engdawork E. 2015. Experimental evaluation of analgesic and anti-inflammatory activity of 80% methanolic leaf extract of Moringa stenopetala Bak. F. in mice. Ethiopian Pharmaceutical Journal 31(1), 15-26.

Ghasemzadeh A, Jaafar HZ. 2013. Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifoliusRoxb.) extracts from different locations of Malaysia. BMC complementary and alternative medicine 13, 1-9.

Halliwell B, Gutteridge JMC. 2007. Free Radicals in Biology and Medicine, Vol 4. Clarendon, Oxford University Press, New York.

Harrison D, Griendling KK, Landmesser U, Hornig B, Drexler H. 2003. Role of oxidative stress in atherosclerosis. The American journal of cardiology 91(3), 7-11.

Hossen SMM, Khan IN, Sarkar MMI, Jahid MA. 2014. Assessment of Thrombolytic Activity of Five Bangladeshi Medicinal Plants: Potential Source for Thrombolytic Compounds. International Blood Research and Reviews 2(6), 262-269.

Islam AMT, Uddin ME, Chowdhury MAU, Rahman MM, Habib MR, Rahman MA. 2013. In vivo antidiarrheal and cytotoxic potential of different fractions of Pandanus Foetidus leaves. American Journal of Biomedical Sciences 5(3), 208-16.

Jain R, Jain SK. 2011. Total Phenolic Contents and Antioxidant Activities of Some Selected Anti-cancer Medicinal Plants from Chhattisgarh State, India. Pharmacology Online 2, 755-762.

Jain S, Sinha A, Bhakuni DS. 2002. The biosynthesis of beta- carboline and quinolizidine alkaloids of Alangium lamarckii. Phytochemistry 60, 853-859.

Khatun MH, Islam MR, Mamun A, Nahar L, Luthfunnesa, Islam MAU. 2011. In Vivo Evaluation of CNS Depressant and Antinociceptive Activities of Methanol Extract of Hibiscus sabdariffa Fruits. Journal of Applied Sciences Research 7(6), 798-804.

Kin A, Yaki LM, Abubakar I, Olusola LF, Zubairu R. 2018. Antibacterial activity of Ocimum gratissimum (scent leaf) on some pathogenic gastrointestinal bacteria. African Journal of. Microbiology Research 12, 923 – 929.

Konate K, Bassole IHN, Hilou A, Aworet-Samseny RR, Souza A, Barro N, M’Batchi B. 2012. Toxicity assessment and analgesic activity investigation of aqueous acetone extracts of Sida acuta Burn f. and Sida cordifolia L. (Malvaceae), medicinal plants of Burkina Faso. BMC Complementary and Alternative Medicine 12, 1-11.

Leonard B, Maes M. 2012. Mechanistic explanations how cell-mediated immune activation, inflammation and oxidative and nitrosative stress pathways and their sequels and concomitants play a role in the pathophysiology of unipolar depression. Neuroscience and Biobehavioral Reviews 36, 764–785.

Lokman HM, Monjur-Al-Hossain ASM, Sarkar KK, Hossin A, Rahman MA. 2013. Phytochemical screening and the evaluation of the antioxidant, Total phenolic content and analgesic properties of the plant Pandanus foetidus (family: Pandanaceae). International Research Journal of Pharmacy 4(2), 170-172.

Marzouk B, Marzouk Z, Haloui E, Fenina N, Bouraoui A, Aouni M. 2010. Screening of analgesic and anti-inflammatory activities of Citrullus colocynthis from southern Tunisia. J. Ethnopharmacol 128, 15-19.

Montoro P, Braca A, Pizza C, De Tommasi N. 2005. Structure–antioxidant activity relationships of flavonoids isolated from different plant species. Food chemistry 92(2), 349-355.

Narayana K. 2003. Poisonous and Medicinal Plants: Jayashri Publications, Bangalore, India, Jayashri Publications, 125–126.

Nisar B, Sultan A, Rbab SL. 2017. Comparison of Medicinally Important Natural Products versus Synthetic Drugs-A Short Commentary. Natural Products Chemistry and Research 6, 308.

Nishikawa H, Hata T, Itoh E, Funakami Y. 2004. A role for corticotropin-releasing factor in repeated cold stress-induced anxiety-like behavior during forced swimming and elevated plus-maze tests in mice. Biological and Pharmaceutical Bulletin 27(3), 352-356.

Nurunnahar, Haque MU, Zahan R, Islam M, Mosaddik A. 2017. Evaluation of Antioxidant Potentiality of Methanolic and Aqueous Extracts of Pandanus foetidus R. Leaves. Journal of Complementary and Alternative Medical Research 3(1), 1-6.

Nurunnahar, Mosaddik A, Zahan R, Maniruzzaman M, Uddin MH, Rahman MA, Khatun S. 2023. Analgesic, anti-inflammatory and CNS depressant activities of Pandanus foetidusRoxb. Leave. International Journal of Pharmaceutical Research and Applications 8, 974-980.

Otieno JN, Hosea KMM, Lyaruu HV, Mahunnah RLA. 2008. Multi-plant or single-plant extracts, which is the most effective for local healing in Tanzania? African Journal of Traditional, Complementary and Alternative Medicines 5(2), 165-172.

Pasco JA, Nicholson GC, Williams LJ, Jacka FN, Henry MJ, Kotowicz MA, Berk M. 2010. Association of high-sensitivity C-reactive protein with de novo major depression. The British Journal of Psychiatry 197(5), 372-377.

Patel AK, Manigauha A. 2018. Antioxidant and antidiabetic activity of isolated flavonoids from Alangium salvifolium leaves extracts. International Journal of Green Pharmacy (IJGP) 12(2), 82-90.

Paul TR, Islam B, Nurunnahar, Khanam K, Wahed MII, Mosaddik A. 2022. Free radical scavenging power potentiates analgesic, anti-inflammatory and cns depressant activity of alangium salviifolium wang, International Journal of Pharmaceutical Sciences and Research 13(10), 4102-4112.

Paul W, Sherman PW, Billing J. 1999. Darwinian Gastronomy:Why We Use Spices: Spices taste good because they are good for us. Bioscience 49, 453-463.

Prajapati SK, Manigauha A, Dubey B. 2019. Anti-inflammatory and Anti-arthritis Activity of Active Constituents Present in Alangium salvifolium Leaves Extract. Indian Journal of Research in Pharmacy and Biotechnology (IJRPB) 7(2), 5-17.

Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry 269(2), 337-341.

Rahman MM, Uddin ME, Islam AMT, Chowdhury MAU, Rahman MA. 2015. CNS depressant and antinociceptive effects of different fractions of Pandanus foetidus Roxb. leaf extract in mice. The Malaysian journal of medical sciences: MJMS 22(3), 33.

Rahman T, Hosen I, Islam MMT, Shekhar HU. 2012. Oxidative stress and human health. Advances in Bioscience and Biotechnology 3, 997–1019.

Rates SMK. 2001. Plants as source of drugs. Toxicon 39(5), 603-613.

Rios-Arrabal S, Artacho-Cordon F, Leon, J, Roman-Marinetto E, del Mar Salinas-Asensio M, Calvente I, Nunez MI. 2013. Involvement of free radicals in breast cancer. Springerplus 2, 1-12.

Salazar R, Pozos ME, Cordero P, Perez J, Salinas MC, Waksman N. 2008. Determination of the antioxidant activity of plants from Northeast Mexico. Pharmaceutical Biology 46(3), 166-170.

Sengar N, Joshi A, Prasad SK, Hemalatha S. 2015. Anti-inflammatory, analgesic and anti-pyretic activities of standardized root extract of Jasminum sambac. Journal of ethnopharmacology 160, 140-148.

Sharma A, Bhatia S, Kharya MD, Gajbhiye V, Ganesh N, Namdeo AG, Mahadik KR. 2010. Anti-inflammatory and analgesic activity of different fractions of Boswellia serrata. International Journal of Phytomedicine 2(1), 94-99.

Shravya S, Vinod BN, Sunil C. 2017. Pharmacological and phytochemical studies of Alangium salvifolium Wang. – A review. Bulletin of Faculty of Pharmacy, Cairo University 55(2), 217-222.

Sikder MAA, Sharmin T, Rahman MAFM, Haque MR, Rahman MS, Rashid MA. 2013. Screenings of four medicinal plants of Bangladesh for bioactivities. Dhaka University Journal of Pharmaceutical Sciences 12(1), 59-62.

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3), 144-158.

Subedi NK, Rahman SM, Akbar MA. 2016. Analgesic and antipyretic activities of methanol extract and its fraction from the root of Schoenoplectus grossus. Evidence-Based Complementary and Alternative Medicine, 3820704.

Sun J, Chu YF, Wu X, Liu RH. 2002. Antioxidant and antiproliferative activities of common fruits. Journal of Agriculture and Food Chemistry 50, 7449-7454.

Tanwer BS, Vijayvergia R. 2014. Biological evaluation of Alangium salvifolium (LF) Wangerin. Journal of Chemical and Pharmaceutical Research 6(12), 611-618.

Uddin SJ, Shilpi JA, Rahman MT, Ferdous M, Rouf R, Sarker SD. 2006. Assessment of neuropharmacological activities of Pandanus foetidus (Pandanaceae) in mice. Die Pharmazie-An International Journal of Pharmaceutical Sciences 61(4), 362-364.

Uddin SJ, Rouf R, Shilpi JA, Alamgir M, Nahar L, Sarker SD. 2008. Screening of some Bangladeshi plants for in vitro antibacterial activity. Oriental Pharmacy and Experimental Medicine 6, 316-321.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry and cell biology 39(1), 44-84.

Warrier PK, Nambiar VPK, Ramankutty C. 1993. Indian Medicinal Plants, a compendium of 500 species. Orient Longman Pvt. Ltd, Madras 5, 1-6.

Williamson EM. 2001. Synergy and other interactions in phytomedicines. Phytomedicine 8(5), 401-409.

Zahan R, Alam MB, Islam MS, Sarker GC, Chowdhury NS, Hosain SB, Mosaddik MA, Jesmin M, Haque ME. 2011. Anticancer activity of Alangium salvifolium flower in Ehrlich Ascites carcinoma bearing mice.  International Journal of Cancer Research 7(3), 254-262.

Zahan R, Anamul HM, Nahar L, Mosaddik A, Haque E. 2012. Evaluation of anxiolytic and CNS depressant activity of Alangium salviifilium wang flowers. International Research Journal of Pharmacy, 3(4), 2230-8407.

Zahan R, Nahar L, Nesa ML. 2013. Antinociceptive and anti-inflammatory activities of flower (Alangium salvifolium) extract. Pakistan Journal of Biological Sciences: PJBS 16(19), 1040-1045.

Zahan R, Nahar L, Haque M, Nesa ML, Alam Z. 2013. Antioxidant and antidiabetic activities of Alangium salvifolium and Bombax ceiba. Dhaka University Journal of Pharmaceutical Sciences, 12(2), 159-163.

Zhu F, Ma XH, Qin C, Tao L, Liu X, Shi Z, Zhang CL, Tan CY, Chen YZ, Jiang Y. 2012. Drug Discovery Prospect from Untapped Species: Indications from Approved Natural Product Drugs. PLOS ONE 7(7), e39782.

Submit Manuscript