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Antioxidant, analgesic, anti-inflammatory and CNS depressant activities of ethanolic and combined extracts of Pandanus foetidus and Alangium salvifolium: In vitro and In vivo studies

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Research Paper 03/09/2023
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Antioxidant, analgesic, anti-inflammatory and CNS depressant activities of ethanolic and combined extracts of Pandanus foetidus and Alangium salvifolium: In vitro and In vivo studies

Nurunnahar, Badrul Islam, Shikha Khatun, Akteruzzaman Chowdhury, Ashik Mosaddik, Imtiaj Hasan
Int. J. Biosci.23( 3), 52-68, September 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: AnalgesicAnti-inflammatoryCNS- depressantCombined extract

Abstract

The present study investigated the phytochemical, antioxidant and in vivo biological activities such as anti-inflammatory, analgesic and CNS depressant activities of the ethanolic extracts of Pandanus foetidus (leaves), Alangium salvifolium (leaves and flowers) and combined extract of these plant species. In the DPPH radical scavenging activity assay, IC50 value of 28.5 µg/ml was found for the combined extract whereas the total antioxidant capacity (0.94±0.3) of that extract was concentration-dependent. All the extracts contained polyphenols and flavonoids but higher values of total phenolic (33.33 ± 5.06 mg/g) and total flavonoid (318.4 ± 4.39 mg/g) content were determined for the combined extract. The combined extract showed higher writhing inhibition (81%; 100mg/Kg body weight) among all the extracts which is quite comparable to the standard drug diclofenac sodium (87.17%; 10mg/Kg body weight). Anti-inflammatory activity of all the extracts was found to be moderate, but the combined extract showed higher activity (87.5% at 100 mg/kg body weight) in mice when compared to the standard (91.25% at 10 mg/kg body weight). The CNS depressant activity of combined extract was 39.75±2.63 at 90 min after administration of the extract, which was 28.25±1.46 for the standard. Our findings suggested that the combined extract of these species can be used as an herbal remedy in pain, inflammatory and CNS disorders. This is the first time that phytochemical and pharmacological activities of combination of extracts of these plant species have been reported.

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