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Antioxidant, Antimicrobial and Antileishmanial Study of Different Parts of Peganum harmala

Zainab Gul Kanwal, Abdul Hafeez, Ihsan Ul Haq, Tofeeq-Ur-Rehman, Syed Aun Muhammad, Irum Shazadi, Nighat Fatima, Nisar Ur Rehman

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Int. J. Biosci.9(1), 45-58, July 2016

DOI: http://dx.doi.org/10.12692/ijb/9.1.45-58

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Abstract

Peganum harmala is bright green, densely foliaged and herbaceous plant, used in folkloric medicine for the treatment of various diseases like asthma, jaundice and lumbago. It contains biologically active secondary metabolites of diverse chemical nature. Therefore present research was designed to determine antioxidant, antimicrobial and antileishmanial potential and phytochemical analysis of different parts of P. harmala. Two plant parts seeds and leaves were selected for comprehensive investigation. According to the results, P. harmala seeds extract showed potent antioxidant activity with IC50 values ranging between 40-129 µg/ml. In case of antibacterial assay, P. harmala seeds showed better inhibitory activity than leaves against both strains i.e. Staphylococcus aureus and Pseudomonas aeruginosa with values ranging between 70 to 100% while in case of antifungal assay water-acetone extract of seeds showed significant antifungal effect against Aspergillus niger. In terms of cytotoxic assay, hexane extract of seeds of were more cytotoxic against shrimp larvae (LD50 = 57.07 µg/ml). Aqueous extract of leaves of and acetone extract of seeds showed < 80% mortality in antileishmanial assay. GC-MS analysis revealed that leaves and seeds contain some important biological metabolites. It is concluded that selected plants could be a potential source of antileishmanial, antibacterial, antifungal and anticancer lead compound. Hence it is indicated to further investigate this plant in vitro as well as in vivo for new drug discovery.

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Antioxidant, Antimicrobial and Antileishmanial Study of Different Parts of Peganum harmala

Akhter N, Haq UI, Mirza B. 2015. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian Journal of Chemistry. http://dx.doi.org/10.1016/j.arabjc.2015.01.01.3

Arshad N, Zitterl-Eglseer K, Hasnain S, Hess M. 2008. Effect of Peganum harmala or its beta-carboline alkaloids on certain antibiotic resistant strains of bacteria and protozoa from poultry. Phytotherapy Research 22(11), 1533-8. http://dx.doi.org/10.1002/ptr.2528.

Baghiani A, Djarmouni M, Boumerfeg S, Trabsa1 H, Charef N, Khennouf S, Arrar L. 2012. Xanthine Oxidase Inhibition and Antioxidant Effects of Peganum harmala Seed Extracts. Europeon Journal of Medicinal Plants. 2(1), 42-56, 2012.

Baker JT, Borris RP, Carte B, Cordell GA, Soejarto DD, Cragg GM, Gupta MP, Iwu MM, Madulid DR, Tyler VE. 1995. Natural products drug discovery and development: new perspectives on international collaboration. Journal of Natural Products. 58(9), 1325 – 1357.

Balick MJ, Cox PA. 1997. Plants, People, and Culture: the Science of Ethnobotany. Scientific American Library, New York, NY.

Benyounes NB, Dahmene T. 2013. The Antimicrobrial Effect of Alkaloids (Harmin, Harmalin) Extracted from Peganum Harmala (L) Seeds in the South of Algeria (Bousaada). International Conference on Sustainable Environment and Agriculture. 57(22), 115-118. http://dx.doi.org/10.7763/IPCBEE.

Bukhari N, Choi JH, Jeon CW, Park HW, Kim WH, Khan MA, Leet SH. 2008. Phytochemical Studies of the Alkaloids from Peganum Harmala. Applied Chemistry 12(1), 101-104.

Butler MS. 2004. The role of natural product chemistry in drug discovery. Journal of Natural Products.67(12), 2141-2153. http://dx.doi.org/10.1021/np040106y

Veerabahu C, Meenakshi VK, Roselin KF. 2013. Infra red and gas chromatogram / mass spectral studies on ethanolic extract of Didemnum psammathodes. Journal of Chemical Current and Pharmaceutical Science 3(3), 196-202.

Chabir N, Ibrahim H, Romdhane H, Valentin A, Moukarzel B, Mars M, Bouajila J. 2014. Seeds of Peganum Harmala L. chemical analysis, antimalarial and antioxidant activities, and cytotoxicity against human breast cancer cells. Medicinal Chemistry. 11(1), 94-101.

Clarke G, Ting NK, Wiart C, Fry J. 2013. High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest. Antioxidants. 2(1), 1-10. http://dx.doi.org/10.3390/antiox2010001

Dai J, Mumper RJ. 2010. Plant Phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 15, 7313-7352. http://dx.doi.org/10.3390/molecules15107313

Darabpour E, Poshtkouhian BA, Motamedi H, Seyyed NSM. 2011. Antibacterial activity of different parts of Peganum harmala L. growing in Iran against multi-drug resistant bacteria. EXCLI Journal. 10, 252-263.

Dastagir G, Hussain F. 2014. Cytotoxic activity of plants of family Zygophyllaceae and Euphorbiaceae. Pakistan Journal of Pharmaceutical Sciences. 27(4), 801-5.

Diba K, Shoar GM, Sharbatkhori M, Khorshivand Z. 2011. Antifungal activity of alcoholic extract of Peganum harmala seeds. Journal of Medicinal Plant Research. 5, 5550-5554. http://dx.doi.org/10.5897/JMPR12.659

Glasby  JS.  1978.  Encyclopedia  of  the  alkaloids. London: Plenum Press. 58-661.

Goel N, Singh N, Saini R. 2009. Efficient in vitro multiplication of Syria Rue (Peganum harmala L.) using 6-benzylaminopurine preconditioned seedling explants. Nature and Science 7, 129-134.

Hamayun M, Hussain F, Afzal S, Ahmad N. 2005. Allelopathic effects of Cyperus rotunds and Echinochloa crus-galli on seed grermination and plumule and radical growth in maize (Zea mays L.). Pakistan Journal of Weed Sciences Research. 11, 81-84.

Hassim N,  Markom M,     Anuar N,  Dewi KH. 2015. Antioxidant and Antibacterial Assays on Polygonum minus Extracts: Different Extraction Methods. International Journal of Chemical Engineering. 1-10. http://dx.doi.org/10.1155/2015/826709

Irshaid FI, Tarawneh KA, Jacob JH, Alshdefat AM. 2014. Phenol content, antioxidant capacity and antibacterial activity of methanolic extracts derived from four Jordanian medicinal plants. Pakistan Journal of Biological Sciences. 17, 372-79.

Kadry N, Khalik A. 2012. A numerical taxonomic study of the family Zygophyllaceae from Egypt. Acta Botanica. Brasilica. 26(1). http://dx.doi.org/10.1590/S010233062012000100017

Kamel S, Ibrahim L, Afifi A, Hamza S. 1970. Major alkaloidal constituents of the Egyptian plant. Peganum harmala. UARJ, Veterinary Sciences. 7, 71-86.

Kaskoos RA. 2014. Physico-chemical Parameters, Phytochemical Screening and Antioxidant Activity of Seeds of Peganum harmala Collected from Iraq. Asian Journal of Biomedical and Pharmaceutical Sciences. 4(28), 20-24.

Khaled HK, Masmoudi H, Ellouz F, ElFeki A, Carreau S. 2008. Protective effects of Peganum harmala extracts on thiourea-induced diseases in adult male rat. Journal of Environmental Biology. 29(1), 73-77.

Khan MJ, Baloch NU, Nabi S, Ahmed N, Bazai Z, Yasinzai M , Al-Kahraman1 YMSA.2012. Antileishmanial, cytotoxic , antioxidant activities and phytochemical analysis of Rhazya stricta Decne leaves extracts and its fractions. Asian Journal of Plant Science and Research, 2(5), 593-598.

Khawar KM, Ozel CA, Balci S, Ozcan S, Arslan O. 2005. Efficient shoot regeneration in Syrian rue (Peganum harmala L.) under in vitro Conditions. International Journal of Agricultural Biology. 7(5), 790-793.

Kim JS, Kwon CS, Son KH. 2000. Inhibition of alpha-glucosidase and amylase by luteolin, a flavoinoid. Biosciences Biotechnology Biochemicals. 64, 2458-2461.

Krishnan K, Mani A. Jasmine, S. 2014. Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8. International Journal of Molecular and Cellular Medicine 3(4), 246–254.

Krueger RJ. 2005. Drugs of Natural Origin. A Textbook of Pharmacognosy. 5th Edition By Gunnar Samuelson. Swedish Pharmaceutical Press, Stockholm. 620 pp. 17 × 25 cm. $70.00. Journal of Natural Products 68(4), 631–631 P. http://dx.doi.org/10.1021/np058229l

Lala S, Pramanick S, Mukhopadhyay S, Bandyopadhyay S, Basu MK. 2004. Harmine: evaluation of its antileishmanial properties in various vesicular delivery systems.Journal of Drug Targeting. 12. (3), 165-75. http://dx.doi.org/10.1080/10611860410001712696

Lamchouri F, Zemzami M, Jossang A, Abdellatif A, Israili ZH, Lyoussi B.  2013. Cytotoxicity of alkaloids isolated from Peganum harmala seeds. Pakistan Journal of Pharmaceutical Sciences. 26(4), 699-706.

Laughlin MC, Chang JL, Smith D. 1991. Bench-top bioassays for the discovery of bioactive natural products: an update. Studies in Natural Products Chemistry 9, 383-409.

Mahmoudian M, Jalilpour H, Salehian P. 2002. Toxicity of Peganum harmala: Review and a case report. Iranian Journal Pharmacological Therapy. 1, 1–4.

Marasini BP, Baral P, Aryal P, Ghimire KR, Neupane S, Dahal N, Singh A, Ghimire L, Shrestha K. 2015. Evaluation of antibacterial activity of some traditionally used medicinal plants against human pathogenic bacteria. Biomed Research International. http://dx.doi.org/10.1155/2015/2654.25

Mashreghi M, Niknia S. 2012. The Effect of Peganum harmala and Teucrium polium Alcoholic Extracts on Growth of Escherichia coli O157. Jundishapur Journal of Microbiology 5(3), http://dx.doi.org/10.5812/jjm.3665

Mirzaie M, Nosratabadi SJ, Derakhshanfar AA, Sharifi I. 2007. Antileishmanial activity of Peganum harmala extract on the in vitro growth of Leishmania major promastigotes in comparison to a trivalent antimony drug. Veterinarski Arhivir. 77(4), 365-375.

Misra P, Khaliq T, Dixit A. 2008. Antileishmanial activity mediated by apoptosis and structure-based target study of peganine hydrochloride dihydrate: an approach for rational drug design. Journal of Antimicrobial Chemotherapy 62(5), 998–1002. http://dx.doi.org/10.1093/jac/dkn319

Moloudizargari M, Mikaili P, Aghajanshakeri S, Asghari MH, Shayegh J. 2014. Pharmacological and therapeutic effects of Peganum harmala and its main alkaloids. Pharmacogosy Review. 7(14), 199 http://dx.doi.org/10.4103/0973-7847.120524

Nafisi S, Asghari MH, Mohammad Nezhadi MA, Soleimani ekhtiari M. 2011. Possible antidiabetic effect of Peganum harmala on streptozocine-induced mouse. Journal of World Applied Sciences 14(6), 822-824.

Nasir E, Ali SI. 1972. Flora of Pakistan. An annotated catalogue of vascular plants of West Pakistan.

Nenaah G. 2010. Antibacterial and antifungal activities of (beta)-carboline alkaloids of Peganum harmala (L) seeds and their combination effects. Fitoterapia. 81(7), 779-82. http://dx.doi.org/10.1016/j.fitote.2010.04.004.

Newman DJ, Cragg GM, Snader KM. 2000. The influence of natural products upon drug discovery. Natural Product Reports. 17(3), 215 – 234.

Newman DJ, Cragg GM, Snader KM. 2003. Natural products as sources of new drugs over the period 1981 – 2002. Journal of Natural Products. 66 (7), 1022 – 1037.

Reza RVM, Abbas H. 2007. Cytotoxicity and Anti-microbial activity of Harman Alkaloids. Journal of Pharmacology and Toxicology 2(7), 677-680. http://dx.doi.org/10.3923/jpt.2007.677.680

Sarpeleh A, Sharifi K, Sonbolkar A. 2009. Evidence of antifungal activity of wild rue (Peganum harmala L.) on phytopathogenic fungi. Journal of Plant Diseases and Protection. 116(5), 208-213.

Shao H, Huang X, Zhang Y, Zhang C. 2013. Main Alkaloids of Peganum harmala L. and Their Different Effects on Dicot and Monocot Crops. Molecules. 18, 2623-2634. http://dx.doi.org/10.3390/molecules18032623

Subashri B, Justin Koil Pillai Y. 2014. A comparitive study of antioxidant activity of Bacopa monnieri (l.) Pennell using various solvent extracts and its gc-ms analysis. International Journal of Pharmacy and Pharmaceutical Sciences. 6(2), 494-498.

Tatiya AU, Tapadiya GG, Kotecha S, Surana SJ. 2011. Effect of solvents on total phenolics, antioxidant and antimicrobial properties of Bridelia retusa Spreng. Stem bark. Indian Journal of Natural Product Research 2(4), 442-447.

Haq UI, Ullah N, Bibi G, Kanwal S, Ahmad SM, Mirza B. 2012. Antioxidant and cytotoxic activities and phytochemical analysis of Euphorbia wallichii root extract and its fractions. International Journal of Pharmaceutical Research. 11(1), 241.

Wang S, Wang Q, Wang Y, Liu L, Weng X, Li G, Zhang X, Zhou X. 2008. Novel anthraquinone derivatives: Synthesis via click chemistry approach and their induction of apoptosis in BGC gastric cancer cells via reactive oxygen species (ROS)-dependent mitochondrial pathway. Bioorganic and Medicinal Chemistry Letters. 18, 6505-6508. http://dx.doi.org/10.1016/j.bmcl.2008.10.047

Xiaojin M, Dongliang L, Haishu T, Yan W, Ting W, Yang L, Jie Y, Jianhua Y, Surong S, Fuchun Z. 2013. Purification and characterization of a novel antifungal protein with antiproliferation and anti-HIV-1 reverse transcriptase activities from Peganum harmala seeds. Acta Biochimica et Biophysica Sinica. 45(2), 87-94. http://dx.doi.org/10.1093/abbs/gms094

Yao G, Sebisubi FM, Voo LYC, Ho CC, Tan G, Chang LC. 2011. Citrinin Derivatives from the Soil Filamentous Fungus Penicillium sp. H9318. Journal of Brazilian Chemical Society 22(6), 1125-1129. http://dx.doi.org/10.1590/S01035053201100060001 8.

Yousefi R, Ghaffarifar F, Dalimi A. 2009. The effect of Alkanna tincturia and Peganum harmala extracts on Leishmania major in vitro. Iranian Journal of Parasitology 4, 40-47.

Zhao  T, Ding K, Zhang  L, Cheng  X, Wang C, Wang   Z. 2013. Acetylcholinesterase and Butyrylcholinesterase Inhibitory   Activities of β-Carboline and Quinoline Alkaloids Derivatives from the Plants of Genus Peganum. Journal of Chemical Education. http://dx.doi.org/10.1155/2013/717232

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