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Antispermatogenic effects of seed extract of Caesalpinia bonducella in Swiss mice

Research Paper | April 1, 2018

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Bibarani Tripathy, Surya N. Swain, Manoj K. Panda, Rabi N. Pradhan, Usha R. Acharya

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Int. J. Biosci.12( 4), 23-34, April 2018

DOI: http://dx.doi.org/10.12692/ijb/12.4.23-34


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Ceasalpinia bonducella, a shrub profusely growing in tropical countries of the world, is known for its medicinal efficacy. However, the male antifertility effects of the plant are not at all documented. Hence, the present study is designed to investigate the male antifertility effects of the seed extract taking Swiss mice as the experimental model. The crude ethanolic seed extract of Caesalpinia bonducella was fed to group of sexually matured Swiss mice at a dose of 500 mg/Kg of body weight at every alternate day in such a way that every mice received 10 doses. The corresponding controls were given distilled water at the same dose and time. The experimental group of mice depicted a significant decrease (p≤0.05) in testis weight compared to controls. Sperm count in experimental groups of mice declined significantly (p≤0.0001) compared to controls. But, there was no increase in abnormal sperm count profile, testicular oxidative stress or body weight indicating that, the seed extract does not have any adverse effect in the testicular physiology. Testicular histology of the treated groups indicated intact seminiferous tubules, but, without spermatids. Flow cytometric analysis of the testis indicated cell arrest at G1 with concomitant decline in DNA synthesis at S phase and sign of tumor development. The finding of the study demonstrates that Caesalpinia bonducella seed extract can be used as an oral male contraceptive after specific isolation and modulation of the drug.


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