Applications of DNA barcoding and future directions
Paper Details
Applications of DNA barcoding and future directions
Abstract
DNA barcoding help to recognize the plant based on short, gene sequences in a rapid, accurate, and cost effective manner. Current focus is on the investigation of phytomedicinals and herbal product integrity and authenticity through DNA barcoding with the goal of protecting consumers from potential health risks associated with product substitution and contamination. Recent reports reveal that DNA barcoding can be used for the assignment of unknown specimens to a taxonomic group, authentic identification of phytomedicinals, and in plant biodiversity conservation. Research indicates that there is no single universal barcode candidate for identification of all plant groups. Hence, comparative analysis of plant barcode loci is essential for choosing a best candidate for authenticating particular medicinal plant genus/families. Currently, both chloroplast/nuclear regions are used as universal barcodes for the authentication of phytomedicinals. A recent advance in genomics has further enhanced the progress in DNA barcoding of plants by the introduction of high-throughput techniques like next generation sequencing, which has paved the way for complete plastome sequencing that is now termed as super-barcodes. Hence, current focus is on the investigation of phytomedicinals and herbal product integrity and authenticity through DNA barcoding with the goal of protecting consumers from potential health risks associated with product substitution and contamination. These approaches could improve the traditional ethno-botanical and scientific knowledge of phytomedicinals and their safe use.
Baker T, Pennington RT, Dexter KG, Fine PVA, Fortune Hopkins H, Honorio EN, Huamantupa I, Klitgård BB, Lewis G, de Lima HC, Ashton P, Baraloto C, Davies S, Donoghue MJ, Kaye M, Kress WJ, Lehmann C, Monteagudo A, Phillips OL, Vasquez R. 2017. Maximising synergy among tropical plant systematists, ecologists and evolutionary biologists. Trends in Ecology and Evolution 32, 258-267.
Baraloto C, Hardy OJ, Paine CET, Dexter KG, Cruaud C, Dunning LT, Gonzalez M, Molino J, Sabatier D, Savolainen V, Chave J. 2012. Using functional traits and phylogenetic trees to examine the assembly of tropical tree communities. Journal of Ecology 100, 690-701.
Chase MW, Salamin N, Wilkinson M, Dunwell JM, Kesanakurthi RP, Haider N, Savolainen V. 2005. Land plants and DNA barcodes: Short term and long term goals. Philosophical Transactions of the Royal Society B Biological Sciences 360, 1889-1895.
Erickson DL, Jones FA, Swenson NG, Pei N, Bourg N, Chen W, Davies SJ, Ge X, Hao Z, Howe RW, Huang CL, Larson A, Lum S, Lutz JA, Ma K, Meegaskumbura M, Mi X, Parker JD, Fang Sun I, Wright J, Wolf AT, Ye W, Xing D, Zimmerman JK, Kress WJ. 2014. Comparative evolutionary diversity and phylogenetic structure across multiple forest dynamics plots: A megaphylogeny approach. Frontiers in Genetics 5, 358.
Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W. 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences USA 101, 14812-14817.
Kress WJ, Erickson DL. 2008. DNA barcoding: A windfall for tropical biology. Biotropica 40, 405-408.
Kress WJ, Lopez IC, Erickson DL. 2012. Generating plant DNA barcodes for trees in long term forest dynamics plots. In: WJ Kress, DL Erickson eds. DNA barcodes: Methods and protocols. New York: Humana Press, Springer Science Publishing Media, LLC 441-458.
Naeem M, Hayat M, Qamar AS, Mehmood T, Munir A, Ahmad G, Azmi UR, Faryad MA, Talib MZ, Irfan M, Hussain A, Hayder MA, Ghani U, Mehmood F. 2019. Risk factors, genetic mutations and prevention of breast cancer. Int. J. Biosci. 14(4), 492-496.
Shapcott A, Forster PI, Guymer GP, McDonald WJF, Faith DP, Erickson DL, Kress WJ. 2015. Mapping biodiversity and setting conservation priorities for SE Queensland’s rainforests using DNA barcoding. PLoS ONE 10, e0122164.
Swenson NG. 2012. Phylogenetic analyses of ecological communities using DNA barcode data. In: WJ Kress, DL Erickson eds. DNA barcodes: Methods and protocols. New York: Humana Press, Springer Science Publishing Media, LLC 409-419.
Swenson NG. 2013. The assembly of tropical tree communities the advances and shortcomings of phylogenetic and functional trait analyses. Ecography 36, 264-276.
Valentini A, Pompanon F, Taberlet P. 2009. DNA barcoding for ecologists. Trends in Ecology and Evolution 24, 110-117.
Webb CO, Donoghue MJ. 2005. Phylomatic: Tree assembly for applied phylogenetics. Molecular Ecology Notes 5, 181-183.
Maryam Pakiza, Syed Naeem Sajid, Asim Hussain, Salman Maqsood, Usman Imtiaz, Haris Ali, Saif Ur Rehman, Sayyad Hammad Mahboob, Sidra Ray, Arzoo Rubab, Sadia Zafar, 2019. Applications of DNA barcoding and future directions. J. Biodiv. Environ. Sci., 15(4), 80-85.
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