Welcome to International Network for Natural Sciences | INNSpub

Aquatic pollution assessment using skin tissues of mulley (Wallago attu, Bloch & Schneider, 1801) as a bio-indicator in Kalpani river at District Mardan, Khyber Pakhtunkhwa, Pakistan

Research Paper | February 1, 2015

| Download 24

Iram Alam Sthanadar, Aftab Alam Sthanadar, Benazir Begum, Muhammad Jamal Nasir, Imtiaz Ahmad, Ali Muhammad, Muhammad Zahid, Sana Ullah

Key Words:

J. Bio. Env. Sci.6( 2), 57-66, February 2015


JBES 2015 [Generate Certificate]


Bioaccumulation profile of five heavy metals including lead (Pb), chromium (Cr), cadmium (Cd), Zinc (Zn) and Nickel (Ni) were investigated in the skin tissue of fresh water fish Mulley, Wallago attu, to assess aquatic pollution in Kalpani river at District Mardan, Khyber Pakhtunkhwa Pakistan. Specimens were collected of three different polluted sites of the river during the months of July through October, 2013. The heavy metals concentration was determined by using Perkin Elmer AS 3100 flame atomic absorption spectrophotometer. Pb was not detected in any of the specimens collected from the river while Cd was the highest and Ni was the least accumulated metal for all sampling months and sites. Mean values recorded (wet weight) for Cr, Cd, Zn and Ni were 0.168±0.326 μg g-1, 0.747±1.106 μg g-1, 0.328±0.074 μg g-1 and 0.161±0.156 μg g-1 respectively. The results showed metals bio accumulation in skin of Mulley in order of Cd>Zn>Cr>Ni, with no detection of Pb. For data validation, statistics of Pearson correlation coefficient matrix was calculated (r > 0.5) It was concluded that Cr and Ni were higher than RDA permissible limits and fish skin act as a primary exposed target to aquatic pollutants.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Aquatic pollution assessment using skin tissues of mulley (Wallago attu, Bloch & Schneider, 1801) as a bio-indicator in Kalpani river at District Mardan, Khyber Pakhtunkhwa, Pakistan

Abah J, Ubwa ST, Onyejefu DI, Nomor SA. 2013. Assessment of the levels of some heavy metals in mudfish (Clarias anguillaris) from River Okpokwu, Apa, Benue State, Nigeria. International Journal of Biosciences 3, 142-150.

Ambedkar G, Muniyan M. 2011. Bioaccumulation of some Heavy Metals in the selected five freshwater fish from Kollidam River, Tamilnadu, India. Advances in Applied Science Research 2, 221-225.

Bervoets L, Blust R, Verheyen R. 2001. Accumulation of Metals in the Tissues of Three Spined Stickelback (Gasterosteus aculeatus) from Natural Fresh Water. Ecotoxicology and Environmental Safety 48, 117-127.

Bhuvaneshwari R, Mamtha N, PaneerSelvam B. 2012. Bioaccumulation of Metals in Muscle, Liver And Gills Of Six Commercial Fish Species at Anaikarai Dam of River Kaveri, South India. International Journal of Pharma and Technology Research 2, 0976-4550.

Bremner I. 1978. Cadmium toxicity, Nutritional influences and the role of metallothionein. World Review of Nutrition and Dietitics 32,165-197.

Buhler  DR,  Stokes  RM,  Caldwell  RS.  1977. Tissue accumulation and enzymatic effects of hexavalent chromium in rainbow trout (Salmo gairdneri). Journal of the Fisheries Board of Canada 34, 9-18.

Canli M, Furness RW. 1993. Heavy metals in tissues of the Noay lobster Neplirops norvegicus: effects of sex, size and season. Chemistry and Ecology 8, 19-32.

Chi Q-q, Zhu G-w, Langdon A. 2007. Bioaccumulation of heavy metals in fishes from Taihu Lake, China. Journal of Environmental Sciences 19, 1500-1504.

Copat C, Bella F, Castaing M, Fallico R, Sciacca S, Ferrante M. 2012. Heavy metals concentrations in fish from Sicily (Mediterranean Sea) and evaluation of possible health risks to consumers. Bulletin of Environmental Contamination and Toxicology 88, 78-83.

Dahunsi SO, Oranusi SU, Ishola RO. 2012. Bioaccumulation Pattern of Cadmium and Lead in the Head Capsule and Body Muscle of Clarias gariepinus [Burchell, 1822] Exposed to Paint Emulsion Effluent. Research Journal of Environmental and Earth Sciences 4, 166-170.

Du Preez HH, Steyn GJ. 1992. A preliminary investigation of the concentration of selected metals in the tissues and organs of the tiger fish (Hydrocynus vittatus) from the Olifants River, Kruger National Park, South Africa. Water 18, 131-136.

Dural M, Goksu M, Ozak AA. 2007. Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon. Food Chemistry 102, 415-421.

Dutton  MD,  Majewski  HS,  Klaverkamp  JF. 1988. Biochemical stress indicators in fish from lakes near a metal smelter. 31st Conference, International Association for Great Lakes Research.

Esler R. 1988. Lead hazards to fish, wildlife, and invertebrates: a synoptic review. U.S. Fish and Wildlife Service, Patuxent Wildlife Research Center Laurel, MD 20708. p. 12.

Fonge BA, Tening AS, Egbe AE, Awo EM, Focho DA, Oben PM, Asongwe PM, Zoneziwoh RM. 2011. Fish (Arius heudelotii, Valenciennes 1840) as bioindicator of heavy metals in Douala Estuary of Cameroon. African Journal of Biotechnology 10, 16581-16588.

Honda K, Sahrul M, Hidaka H, Tatsukawa R. 1983. Organ and tissue distribution of heavy metals, and their growth-related changes in Antarctic fish, Pagothenia borchgrevinki. Agricultural and Biological Chemistry 47, 2521-2532.

Karadede Hl, Oymak SA, Unlu E. 2004. Heavy metals in mullet, Liza abu, and catfish, Silurus triostegus, from the Ataturk Dam Lake (Euphrates), Turkey. Environmental International 30, 183-188.

Keepax RE, Moyes LN, Livens FR. 2011. Speciation of heavy metals and radioisotopes, in Environmental and Ecological Chemistry Vol II. Encyclopedia of Life Support Systems (EOLSS).

Mendil D, Uluözlü ÖD. 2007. Determination of trace metal levels in sediment and five fish species from lakes in Tokat, Turkey. Food Chemistry 101, 739-745.

Mirza MR, Sandhu AA. 2007. Fish of the Punjab, Pakistan. Polymer Publishers, Rahatmarket, Urdu Bazar.

Nesto N, Romano S, Moschino V, Mauri M, Da Ros L. 2007. Bioaccumulation and biomarker responses of trace metals and micro-organic pollutants in mussels and fish from the Lagoon of Venice, Italy. Marine Pollution Bulletin 55, 469-484.

Obasohan EE, Oronsaye JAO, Obano EE. 2006. Heavy metal concentrations in Malapterurus electricus and Chrysichthys nigrodigitatus from Ogba River in Benin City, Nigeria. African Journal of Biotechnology 5, 974-982.

Rao LM, Padmaja G. 2000. Bioaccumulation of heavy metals in M. cyprinoids from the harbor waters of Visakhapatnam. Bulletin of Pure and Applied Science 19, 77-85.

Reynders H, Bervoets L, Gelders M, De Coen WM, Blust R. 2008. Accumulation and effects of metals in caged carp and resident roach along a metal pollution gradient. Ecotoxicology and Environmental Safety 391, 82-95.

Sthanadar IA, Sthanada AA, Yousaf M, Muhammad A, Zahid M. 2013a. Bioaccumulation profile of heavy metals in the gills tissue of Wallago attu (MULLEY) from Kalpani River Mardan, Khyber Pakhtunkhwa Pakistan. International Journal of Biosciences 3, 165-174.

Sthanadar IA, Sthanadar AA, Shah M, Asmat P, Yousaf M, Muhammad A, Zahid M. 2013b. White muscle as a bio-indicator of cadmium (Cd) pollution across Kalpani River Mardan, Khyber Pakhtunkhwa Pakistan. International Journal of Biosciences 3, 105-116.

Sthanadar AA, Sthanadar AA, Muhammad A, Ali PA, Shah M, Zahid M, Begum B, Yousaf M. 2013c. Bio accumulation profile of heavy metals in the liver tissues of Wallago attu (MULLEY) from Kalpani River Mardan, Khyber Pakhtunkhwa Pakistan. International Journal of Biosciences 03 (11), 92-103.

Talwar PK, Jhingran AGK. 1991. Inland fishes of India and adjacent countries, CRC Press.

Tanee T, Chaveerach A, Narong C, Pimjai M, Punsombut P, Sudmoon R. 2013. Bioaccumulation of heavy metals in fish from the Chi River, Maha Sarakham Province, Thailand. International Journal of Biosciences 3, 159-167.

Terra BF, Araujo FG, Calza CF, Lopes RT, Teixeira TP. 2008. Heavy metal in tissues of three fish species from different trophic levels in a tropical Brazilian river. Water, Air, and Soil Pollution 187, 275-284.

Tjalve H, Ccottofrey J, Borg K. 1988. Bio accumulation, distribution and retention of Ni2+ in the brown trout, Salmo trutta. Water Research 22, 321-324.

Ullah S, Ahmad T. 2014. Nutritional and Medical Importance of Fish: A Mini Review. Reviews of Progress 2(2), 1-5.

Ullah S, Javed MW, Shafiqu M, Khan SF. 2014. An integrated approach for quality assessment of drinking water using GIS: A case study of Lower Dir. Journal of Himalayan Earth Sciences 47(2), 163-174.

Van der Putte I, Part P. 1982. Oxygen and chromium transfer in perfused gills of rainbow trout (Salmo gairdneri) exposed to hexavalent chromium at two different pH levels. Aquatic Toxicology 2, 31-45.

Van Loon JA. 1980. Analytical atomic absorption spectroscopy: selected methods, Elsevier, New york, USA.337.

Vinodhini R, Narayanan M. 2008. Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). International Journal of Environmental Science and Technology 5, 179-182.

Yalmaz F, Ozdemir N, Demirak A, Tuna AL. 2007. Heavy metal levels in two fish species Leuciscus cephalus and Lepomis gibbosus. Food Chemistry 100, 830-835.

Yang R, Yao T, Xu B, Jiang G, Xin X. 2007. Accumulation features of organochlorine pesticides and heavy metals in fish from high mountain lakes and Lhasa River in the Tibetan Plateau. Environment International 33, 151-156.

Yilmaz AB. 2003. Levels of heavy metals (Fe, Cu, Ni, Cr, Pb, and Zn) in tissue of Mugil cephalus and Trachurus mediterraneus from Iskenderun Bay, Turkey. Turkish Journal of Environmental Research 92, 277-281.

Yousafzai AM, Shakoori AR. 2006. Bioaccumulation of Chromium, Nickle, Lead, Copper and Zinc in the skin of Tor putitora as an indicator of the presence of heavy metal load in River Kabul, Pakistan. Pakistan Journal of Zoology 38, 341-347.

Yousafzai AM, Chivers DP, Khan AR, Ahmad I, Siraj M. 2010. Comparison of heavy metals burden in two freshwater fishes Wallago attu and Labeo dyocheilus with regard to their feeding habits in natural ecosystem. Pakistan Journal of Zoology 42, 537-544.

Zhang Z, He L, Li J, Wu Z-b. 2007. Analysis of heavy metals of muscle and intestine tissue in fish-in Banan section of Chongqing from three gorges reservoir, China. Polish Journal of Environmental Studies 16, 949-958.