Assessment and molecular characterization of citrus canker causing pathotypes
Paper Details
Assessment and molecular characterization of citrus canker causing pathotypes
Abstract
Citrus is one of the most popular and significant commercial fruit of Pakistan. Outbreak of Citrus Canker diseased in different varieties causes enormous economic loss. The present research work was conducted to identify and characterize citrus canker causing Pathotypes in citrus varieties from the orchards of Khanpur, district Haripur and Islamabad (National Agriculture Research Council). The survey was conducted in orchards of Khanpur and Islamabad during March, 2015 to find out the incidence, prevalence, severity and disease index as well as for sampling from selected orchards. Varieties encountered during the survey included Grapefruit, Malta (musambi), Red Blood, Ruby Blood and Lemon. Causal organisms were isolated and grown on nutrient agar (NA) and Potato dextrose agar (PDA) for morphological studies. Firstly, Pathogens were identified on the basis of morphological characters.The incidence of Khanpur and National Agriculture Research Council, Islamabad orchards observed were about 10% and 95% respectively. Complete description of macro and microscopic characters was prepared. The purified cultures of bacterial pathogen was then identified and characterized. The identified microbial pathogens include Xanthomonas citri. DNA was successfully extracted and amplified by using TaKaRa Ex taqTM version kit with primers 9F and 15-10R, to confirm the identification by gene sequence. Identified Strains were stored for further study. It is recommended on basis of present study to carry out further experimentation on pathogenicity of these pathotypesforfinding out the citrus varieties resistance against this disease. There is also need to control this pathogen using different biological/chemical agents and genetic engineering techniques.
Ahmad B, Mustafa K. 2006. Forecasting kinnow production in Pakistan: An econometric analysis. International Journal of Agriculture and Biology 8(4), 455-458.
Ahmed I, Yokota A, Fujiwara T. 2007. A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. Extremophiles 11(2), 217-224.
Baghurst K. 2003. The Health Benefits of Citrus Fruits. Consumer Science Program CSIRO Health Sciences & Nutrition. Horticultural Australia Ltd. PN:CT 02057.
Bertoni G, Mills D. 1987. A simple method to monitor growth of bacterial populations in leaf tissue. Phytopathology 77, 832–835.
Bock CH, Parker PE, Gottwald TR. 2005. Effect of simulated wind-driven rain on duration and distance of dispersal of Xanthomonas axonopodis pv. citri from canker-infected citrus trees. Plant Disease 89(1), 71-80.
Bui-Thi-Ngoc L, Vernière C, Pruvost O, So T, Johnson GI. 2008. First report of Xanthomonas axonopodis pv. citri-A* causing citrus canker on lime in Cambodia. Plant Diseases 92, 1588.
Das AK. 2003. Citrus canker – a review. Journal of Applied Horticulture5, 52–60.
Department of Agriculture, Australian Government. 2014. National Diagnostic Protocol for Asiatic Citrus Canker, Xanthomonas citri subsp. citri. Retrieved from website: https://www.ippc.int/publications/dp-6-2014-xanthomonas- citri-subsp-citri.
Derso E, Sijam K, Ahmad ZM, Omar, Ibrahim, Napis S. 2006. Status of citrus canker caused by Xanthomonas axonopodis pv. citri in Peninsular Malaysia. International journal of Agriculture and Biology 9, 54-58.
Derso TA. 2009. On-farm evaluation of urea treated rice straw and rice bran supplementation on feed intake, milk yield and composition of Fogera cows, North Western Ethiopia.
Fatima S, Anjum T, Bajwa R, Shahid AA. 2012. Identification of indigenous Xanthomonas isolates through 16S rRNA gene amplification and SDS PAGE analyses. African Journal of Microbiology Research 6(15), 3651-3655.
Gottwald T, Graham J, Schubert T. 1997. An epidemiological analysis of the spread of citrus canker in urban Miami, Florida, and synergistic interaction with the Asian citrus leaf miner. Fruits 52, 383–390.
Gottwald TR, Graham JH, Schubert TS. 2002. Citrus canker: The pathogen and its impact. Online. Plant Health Progressdoi: 10.1094/PHP-2002-0812-01-RV.
Gracelin J, De Britto ADHS, Sebastian SR. 2011. Antibacterial activity of a few medicinal plants against Xanthomonas campestris and Aeromon-ashydrophila. Journal of Biopesticides 4(1), 57-60.
Katsivela E, Bonse D, Kruger A, Strompl C, Livingston A, Mittich R. 1999. An extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water. Applied Microbiology and Biotechnology 52, 853–862.
Khan M, Abid M. 2007. Effect of environmental conditions on citrus canker disease development. Pakistan journal of Phytopathology 19, 139–144.
Khan MM, Khan MA, Inam-ul-Haq M, Ahmad R, Aziz I. 1992. Incidence of citrus canker caused by X. campestrispv.citri orchard in Faisalabad District. In: Proceed. 1st Inter. sem. citriculture in Pakistan. Dec. 2-5. University of Agriculture Faisalabad.311-314.
Lakshmi TN, Gopi V, Sankar TG, Sarada G, Lakshmi LM, Ramana KTV, GopalK. 2014. Status of Diseases in Sweet Orange and Acid Lime Orchards in Andhra Pradesh, India. International Journal of Current Microbiology and Applied Sciences 3(5), 513-518.
Ngugi HK, King SB, Abayo GO, Reddy YVR. 2002. Prevalence, incidence and severity of sorghum diseases in Western Kenya. Plant Diseases 86, 65– 70.
P k č A Gaš č K Iva v č MM K zma v č Š v č M P lawska J & Ob ad v č A. 2012. Detection and Identification Methods and New Tests as Developed and used in the framework of cost873 for Bacteria Pathogenic to Stone Fruits and Nuts. Journal of Plant Pathology 94(1), 127.
Pruvost O, Boher B, Brocherieus C, Nicole M, Chiroleu F. 2002. Survival of Xanthomonas axonopodis pv. citri in leaf lesions under tropical environmental conditions and simulated splash dispersal of inoculum. Phytopathology 92, 336-346.
Qureshi MN, Bial M, Al MK, Ayyub RM. 2014. Modeling Of Citrus Fruits Production In Pakistan. Science International 26(2).
Roberts PD, Chamberlain HL, Chung KR, Schubert TS, Graham JH, Timmer LW. 2005. Florida citrus pest management guide: citrus canker.
Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution 4(4), 406-425.
Singh D, Thind SK. 2014. Prevalence, isolation and standardization of growth media for Xanthomonas axonopodis pv. citri causing citrus canker. Plant Disease Research 29(2), 188-192.
Sun X, Stall RE, Jones JB, Cubero J, Gottwald TR, Graham JH, Wayne DN, Schubert ST, Paul SH, Verlyn SK, Gorge LH, Sutton BD. 2004. Detection and characterization of a new strain of citrus canker bacteria from Key/Mexican lime and alemow in South Florida.Plant Disease 88(11), 1179-1188.
Verniere C, Hartung JS, Pruvost OP, Civerolo EL, Alvarez AM, Maestri P, Luisetti J. 1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from Southwest Asia. European Journal of Plant Pathology 104(5), 477-487.
Verniere CJ, Gottwald TR, Pruvost O. 2003. Disease development and symptom expression of Xanthomonas axonopodis pv. citri in various citrus plant tissues. Phytopathology 93(7), 832-843.
Sumyia Iqbal, Shazia Iftikhar, Anam Rasool (2016), Assessment and molecular characterization of citrus canker causing pathotypes; IJAAR, V8, N4, April, P93-100
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