Assessment of divergence in electrophoretic mobility pattern of total seed protein in (Brassica juncea L.) germplasm using SDS-PAGE analysis

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Research Paper 01/10/2015
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Assessment of divergence in electrophoretic mobility pattern of total seed protein in (Brassica juncea L.) germplasm using SDS-PAGE analysis

Noor Saleem, Naushad Ali, Malik Ashiq Rabbani
Int. J. Biosci.7( 4), 94-101, October 2015.
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Abstract

Genetic diversity of one hundred local Indian mustard (Brassica juncea L.) accessions was characterized for total seed storage protein via sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). The accessions used in the study were obtained from Gene bank of Plant Genetic Resources Institute (PGRI), National Agricultural Research Center (NARC), (Latitude 33.420 N; Longitude 73.080 E) Islamabad Pakistan. Total seed proteins were resolved on 12.25 % polyacrylamide gels generating a total of 21 bands on the basis of molecular weight within the range of 6 to ≈180 kDa. Among them seventeen bands (80.95%) were polymorphic and the remaining 4 (19.04%) were monomorphic showing high degree of variability viz- à -viz peptide mobility for studied germplasm. Similarity index among these accessions ranged from 0.62 to 1.0. Protein dissimilarity based dendrogram was generated through un-weighted pair group method with arithmetic average (UPGMA) which distributed all hundred accessions into 5 main clusters. Grouping pattern revealed moderate level of genetic divergence in seed protein profiling however it is suggested that 2-D gel-electrophoresis with other molecular techniques should be applied in future to explore genetic variation because only SDS-PAGE of seed protein is insufficient to completely estimate the genetic diversity present among these accessions.

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