Welcome to International Network for Natural Sciences | INNSpub

Assessment of epidemiological and hematological parameters of dengue virus infection in District Mardan, Khyber Pakhtunkhwa, Pakistan

Research Paper | August 1, 2019

| Download 5

Shah Faisal, Hasnain Jan, Muhammad Haroon, Yasir Khaqan Habib, Farhan Ahmad, Ajmal Khan

Key Words:

Int. J. Biosci.15( 2), 274-280, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.274-280


IJB 2019 [Generate Certificate]


Dengue is a tropical disease caused by dengue virus (DENV). It is one of the most significant arthropod-borne viral infection. Dengue fever has been reported from various parts of Pakistan. However, there is still a scarcity of data on the prevalence and incidence of dengue. The aim of the present study was to determine the seroprevalence and hematological changes in dengue virus-positive patients in district Mardan, Khyber Pakhtunkhwa (KP). In the current study, 1000 suspected individuals including 632 male and 368 female samples were collected from different areas of district Mardan. Out of 632 male individual samples, 88 were positive while 46 out of 368 female samples were found positive for dengue infection. The primary and secondary infection was determined through IgM (M=49, F=21) and IgG (M=19, F=15) ICT, and through NS1 (M=20, F=10) ICT strips. Dengue fever also causes hematological changes in infected patients. The complete blood count (CBC) was determined for patients which undergoes hypo-volumatic shock condition. In the infected individuals low TLC, low PLT, high Hb and high HCT values were observed. In the current study, the frequency of infection was higher in male as compared to female. It was also concluded that the dengue infection can be early diagnosed on the basis of described clinical features and with the detection of dengue-specific NS1 antigen along with antibodies such as IgG and IgM.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Assessment of epidemiological and hematological parameters of dengue virus infection in District Mardan, Khyber Pakhtunkhwa, Pakistan

Ali A, Ahmad H, Idrees M, Zahir F, Ali I. 2016. Circulating serotypes of dengue virus and their incursion into non-endemic areas of Pakistan; a serious threat. Virology journal 13(1), 144. https://doi.org/10.1186/s12985-016-0603-6

Cardier JE, Mariño E, Romano E, Taylor P, Liprandi F, Bosch N, Rothman AL. 2005. Proinflammatory factors present in sera from patients with acute dengue infection induce activation and apoptosis of human microvascular endothelial cells: possible role of TNF-α in endothelial cell damage in dengue. Cytokine 30(6), 359-365. https://doi.org/10.1016/j.cyto.2005.01.021

Chen RF, Yang KD, Wang L, Liu JW, Chiu CC, Cheng JT. 2007. Different clinical and laboratory manifestations between dengue haemorrhagic fever and dengue fever with bleeding tendency. Transactions of the Royal Society of Tropical Medicine and Hygiene 101(11), 1106-1113. https://doi.org/10.1016/j.trstmh.2007.06.019

Diamond MS, Pierson TC. 2015. Molecular insight into dengue virus pathogenesis and its implications for disease control. Cell 162(3), 488-492. https://doi.org/10.1016/j.cell.2015.07.005

Fontenille D, Failloux AB, Romi R. 2007. 10. Should we expect Chikungunya and Dengue in Southern Europe? Emerging pests and vector-borne diseases in Europe, 169.

Guzman MG, Kouri G. 2003. Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges. Journal of Clinical Virology 27(1), 1-13. https://doi.org/10.1016/S1386-6532(03)00010-6

Halstead SB. 1988. Pathogenesis of dengue: challenges to molecular biology. Science 239(4839), 476-481. http://dx.doi.org/10.1126/science.239.4839.476

Haroon M, Jan H, Faisal S, Ali N, Kamran M, Ullah F. 2018. Dengue Outbreak in Peshawar: Clinical Features and Laboratory Markers of Dengue Virus Infection. Journal of infection and public health. https://doi.org/10.1016/j.jiph.2018.10.138

Hesse RR. 2007. Dengue virus evolution and virulence models. Clinical Infectious Diseases 44(11), 1462-1466. https://doi.org/10.1086/517587

Srichaikul T, Nimmannitya S. 2000. Haematology in dengue and dengue haemorrhagic fever. Best Practice & Research Clinical Haematology 13(2), 261-276. https://doi.org/10.1053/beha.2000.0073

Tsai JJ, Chang JS, Chang K, Chen PC, Liu LT, Ho TC, Tan SS, Chien YW, Lo YC, Perng GC. 2017. Transient monocytosis subjugates low platelet count in adult dengue patients. Biomedicine Hub 2(1), 8-8. https://doi.org/10.1159/000457785