Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers
Paper Details
Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers
Abstract
This study was conducted to assess the genetic diversity and relationship of 19Thai Musa accessions of four (ABB, AAA, AA, and BB) genomes based on Random Amplified Polymorphic DNA (RAPD) and Sequence Related Amplified Polymorphism (SRAP) markers. The results revealed that thirteen RAPD and eight SRAP markers generated a total of 129 alleles (calculated from 1005 amplified fragments), with sizes ranging from 100 to 2100 bp long. Moreover, both markers provided high polymorphism with average PIC value of 0.75, which indicated a high level of genetic diversity among studied Musa accessions. The UPGMA approach based grouping showed two distinct clusters, which were cluster-I comprised of ABB, AA, and BB genomes and cluster-II consisted of only the AAA genome. Additionally, its analysis demonstrated high genetic relationship within the ABB genome, compared to other (AAA, AA, and BB) genomes. This study also confirmed that both RAPD and SRAP markers were proficient tools for rapidly accessing the genetic diversity within all the banana genomic groups.
Abdelhamid A, El-Shahed, Abdellatif KF, Ibrahim IA, Mohamed AM, Abdelsalam IZ, Elsehrawy OA. 2017. Efficiency of the new SRAP and TRAP markers to detect the banana somaclonal variants. Life Science Journal. http://dx.doi.org/10.5897/AJB2017.15893
Bhatt J, Kumar S, Patel S, Solanki R. 2017. Sequence-related amplified polymorphism (SRAP) markers based genetic diversity analysis of cumin genotypes. Annals of Agrarian Science. https://dx.doi.org/10.1016/j.aasci.2017.09.0
Brown N, Khittoo G, Bahorun T, Jawaheer S. 2009. Evalution of genetic diversity between 27 banana cultivars (Musa spp.) in Mauritius using RAPD markers. African Journal of Biotechnology.
Crossa, Warburton. 2002. Data analysis in the CIMMYT applied biotechnology center for fingerprinting and genetic diversity studies. CIMMYT.
Dnyaneshwar W, Preeti C, Kalpana J, Bhushan P. 2006. Development and Application of RAPD-SCAR Marker for Identification of Phyllanthus emblica L. Biological and Pharmaceutical Bulletin.
Espósito MA, Martin EA, Cravero VP, Cointry E. 2007. Characterization of pea accessions by SRAP’s markers. ScientiaHorticulturae 113, 329-335. https://dx.doi.org/10.1016/j.scienta.2007.04.006
FAO. 2017. Banana market review Preliminary results. http://www.fao.org/economic/est/estcommodities/bananas/en/
Kiran U, Khan S, Mirza KJ, Ram M, Abdin MZ. 2010. SCAR markers: a potential tool for authentication of herbal drugs. Fitoterapia. https://dx.doi.org/10.1016/j.fitote.2010.08.002
Kiran U, Moahnty S, Roy P, Behera L, Chand P. 2015. Genetic diversity among banana cultivars from Odisha using RAPD Markers. Science Research Reporter.
Masoud S, Zahra N, Farah F. 2008. RAPD analysis of somaclonal variation in banana(Musa acuminate L.) cultivar valery. ActaBiologicaSzegediensi5.
Ning SP, Xu LB, Lu Y, Huang BZ, Ge XJ. 2007. Genome composition and genetic diversity of Musagermplasm from China revealed by PCR-RFLP and SSR markers. Scientia Horticulturae 114, 281-288. https://dx.doi.org/10.1016/j.scienta.2007.07.002
Nsabimana A, Van Staden J. 2007.Assessment of genetic diversity of Highland bananas from the national banana germplasm collection at Rubona, Rwanda using RAPD markers.ScientiaHorticulturae – Elsevier. https://dx.doi.org/10.1016/j.scienta.2007.04.010
Onguso J, Ndiritu W, Mizutani F.2004.Genetic characterization of cultivated bananas and plantains in Kenya by RAPD markers. ScientiaHorticulturae – Elsevier. http//dx.doi.org/10.1016/S0304-4238(03)00082-7
Opara UL, Jacobson D, Al-Saady N. 2010. Analysis of genetic diversity in banana cultivars (Musacvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses. J Zhejiang Univ Sci B. https://dx.doi.org/10.1631/jzus.B0900310
Ozhan Simsek PC, Fatma Aslan, Melda Bayramoglu, TolgaIzgu, Jaime A. Teixeira da Silva, Yildiz Aka Kacar, Yesim Yalcin Mendi. 2016. Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers. Biochem Genet Cross Mark. http//dx.doi.org/10.1007/s10528-016-9770-9
Pankaj Kumar Jain MLS, HardikPathak, Virendra Kumar Gupta.2007.Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs). African Journal of Biotechnology 6(17). http//dx.doi.org/10.5897/AJB2007.000-2304
Patel HK, Fougat RS, Kumar S, Mistry JG, Kumar M. 2015. Detection of genetic variation in Ocimum species using RAPD and ISSR markers.Biotech 5, 697-707.
Peng L, Ru M, Wang B, Wang Y, Li B, Yu J, Liang Z. 2014. Genetic diversity assessment of a germplasm collection of Salvia miltiorrhiza Bunge based on morphology, ISSR and SRAP markers. Biochemical Systematics and Ecology 55, 84-92. https://dx.doi.org/10.1016/j.bse.2014.01.020
Phothipan S, Silayoi B, Wanichkul K, Apisitwanich S. 2005. Genetic relationship among bananas in AA, AAB and BB groups using random amplified polymorphic DNA (RAPD) and sequence related amplified polymorphism (SRAP) techniques. Kasetsart Journal.
Ploetz RC, Kepler AK, Daniells J, Nelson SC. 2007. Banana and plantain—an overview with emphasis on Pacific island cultivars. Species profiles for Pacific Island agroforestry.
Poerba YS, Ahmad F. 2010. Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11(3), 118-123.
Quiros GLF. 2001.Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Springer-103, 455-461.
Rotchanapreeda T, Wongniam S, Swangpol S, Chareonsap P, Sukkaewmanee N, Somana J. 2016. Development of SSR markers from Musa balbisiana. Plant Systematics and Evolution, Plant Systematics and Evolution.
Ruangsuttapha S, Eimert K, Schröder MB, Silayoi B, Denduangboripant J, Kanchanapoom K. 2007. Molecular phylogeny of banana cultivars from Thailand based on HAT-RAPD markers. Genetic Resources and Crop Evolution.
Santos JRP, Teixeira MA, Cares JE, Faleiro FG, Costa DC. 2009. Contrastant banana accessions for resistance to the burrowing nematode, based on molecular markers RAPD. Euphytica.
Singh WS, Sorokhaibam S, Shrivastava, Karuna. 2014. Analysis of banana genome groups of wild and cultivated cultivars of Manipur, India using sScore card method. Advances in Applied Science Research 5, 35.
Suvittawat K, Teinseree N, Saradhuldhat P. 2014. Growth and yield of eight’namwa'(ABB) banana in Thailand. ISHS Acta Horticulturae. https://dx.doi.org/10.17660/ActaHortic.2014.1024.3
Valquíria Martins Pereira CVB, Lívia Pinto Brandao, Larissa Santos Oliveira, Cintia Paula Feitosa Souza, Zalmar Santana Gonçalves, Sebastião de Oliveira e Silva, Janay Almeida dos Santos-Serejo, Claudia Fortes Ferreir, Edson PeritoAmorim, Carlos Alberto da Silva Ledo. 2012. Genetic diversity between improved banana diploids using canonical variables and the Ward‑MLM method.Pesq.agropec. bras, Brasília. http://dx.doi.org/10.1590/S0100204X2012001000010
Wong C, Kiew R, Loh JP, Gan LH, Set O, Lee SK, Lum S, Gan YY. 2001 .Genetic Diversity of the Wild Banana Musa acuminataColla in Malaysia as Evidenced by AFLP. Annals of Botany 88, 1017-1025. https://dx.doi.org/10.1006/anbo.2001.1542
ŽelmíraBalážová MV, ZdenkaGálová. 2016. Evaluation of molecular diversity of Central European maize cultivars. Emirates Journal of Food and Agriculture. http://dx.doi.org/10.9755/ejfa.2015-05-204
R.O.B. Zozimo, K. Ratanasut, T. Boonsrangsom, K. Sujipuli (2018), Assessment of genetic diversity among thai banana cultivars (Musaspp.) based on RAPD and SRAP markers; IJB, V12, N4, April, P172-180
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